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Methicillin-resistant Staphylococcus aureus: a 6-month survey in a Lisbon paediatric hospital

Published online by Cambridge University Press:  19 October 2009

J. A. G. Melo Cristino ,
A. Torres Pereira ,
F. Afonso  and
J. Naidoo
J. A. G. Melo Cristino
Affiliation:
Instituto Bacteriologico Câmara Pestana, Department of Microbiology, Faculty of Medicine, University of Lisbon
A. Torres Pereira
Affiliation:
Instituto Bacteriologico Câmara Pestana, Department of Microbiology, Faculty of Medicine, University of Lisbon
F. Afonso
Affiliation:
Paediatric Surgical Unit, Hospital D. Estefânia, Lisbon, Portugal
J. Naidoo
Affiliation:
Department of Bacteriology, Institute of Dermatology, University of London, UK
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Summary

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The prevalence of nasal colonization and infection with methicillin-resistant Staphylococcus aureus (MRSA) among patients and staff was studied in a section of a Paediatric Surgical Unit in Lisbon between February and July 1985. Nasal colonization was demonstrated in 41% of burned patients, 5% of non-burned patients and 35 % of the nurses. Infection by MRSA occurred in 30% of the burns.

The isolates had identical serological patterns, slight differences on phage typing and were resistant to methicillin, cephalosporins, tetracycline, erythromycin and aminoglycosides. A chloramphenicol resistance plasmid of 3 Md was present in those isolates which were chloramphenicol resistant and a small plasmid of 1·7 Md which coded for constitutive erythromycin resistance was present in many isolates. Gentamicin, tetracycline and inducible erythromycin resistance were chromosomal.

Several reasons for the apparent low virulence of the isolates are discussed. Attempts to control the outbreak by the discharge of colonized or infected patients, improvement of nursing practices and treatment with temporary removal from work of the colonized nurses did not eliminate the organism from the unit.

Type
Research Article
Information
Journal of Hygiene , Volume 97 , Issue 2 , October 1986 , pp. 265 - 272
Copyright
Copyright © Cambridge University Press 1986

References

Barry, A. L. & Thornsberry, C. (1985). Susceptibility tests: diffusion test procedures. In Manual of Clinical Microbiology, 4th ed. (ed. Lennette, E. H., Balows, A., Hausler, W. J. Jr & Shadomy, H. J.), pp. 978–987. Washington, D.C.: American Society for Microbiology.Google Scholar
Boyce, J. M., White, R L., Causey, W. A. & Lockwood, W. R. (1983). Burn units as a source of methicillin-resistant Staphylococcus aureus infections. Journal of the American Medical Association 249, 2803–2807.Google Scholar
Bradley, M. T., Noone, P., Townsend, D. E. & Grubb, W. B. (1985). Methicillin-resistant Staphylococcus aureus in a London hospital. Lancet i, 1493–1495.CrossRefGoogle Scholar
Cafferkey, M. T., Coleman, D., McGrath, B., Hone, R., Pomeroy, H., Ruddy, R. & Keane, C. T. (1985). Methicillin-resistant Staphylococcus aureus in Dublin 1971–84. Lancet ii, 705–708.CrossRefGoogle Scholar
Collopy, B. T., Dalton, M. F., Wright, C. & Mullany, C (1984). Comparison of the clinical significance of methicillin-resistant and methicillin-sensitive Staphylococcus aureus isolation. Medical Journal of Australia 140, 211–214.CrossRefGoogle Scholar
Cookson, B. D., Farrington, M., Webster, M. & Phillips, I. (1985). Methicillin-resistant Staphylococcus aureus. Lancet ii, 218–219.CrossRefGoogle Scholar
Crossley, K., Landesman, B. & Zaske, D. (1979). An outbreak of infections caused by strains of Staphylococcus aureus resistant to methicillin and aminoglycosides. II. Epidemiologic studies. The Journal of Infectious Diseases 139, 280–287.Google Scholar
Editorial (1985). What's to be done about resistant Staphylococci? Lancet ii, 189–191.Google Scholar
Hone, R., Cafferkey, M., Keane, C. T., Harte-Barry, M., Moorhouse, E., Carroll, R., Martin, F. & Ruddy, R. (1981). Bacteraemia in Dublin due to gentamicin-resistant Staphylococcus aureus. The Journal of Hospital Infection 2, 119–126.CrossRefGoogle ScholarPubMed
Iordanescu, S. & Surdeanu, M. (1980). New incompatibility groups for Staphylococcus aureus plasmids. Plasmid 4, 256–260.CrossRefGoogle ScholarPubMed
Lacey, R. W. (1984). Antibiotic resistance in Staphylococcus aureus and Streptococci. British Medical Bulletin 40, 77–83.Google Scholar
Linnemann, C. C. Jr, Mason, M., Moore, P., Korfhagen, T. R. & Staneck, J. L., (1982). Methicillin resistant Staphylococcus aureus: experience in a general hospital over four years. American Journal of Epidemiology 115, 941–950.CrossRefGoogle Scholar
Melo Cristino, J. A. G., Torres Pereira, A. & Afonso, F. (1985). Infection with methicillingentamicin-resistant Staphylococcus aureus in a paediatric surgical unit in Lisbon. The Journal of Hospital Infection 6, 426–428.CrossRefGoogle Scholar
Pereira, A. T. (1961). Antigenic loss variation in Staphylococcus aureus. The Journal of Pathology and Bacteriology 81, 151–156.CrossRefGoogle ScholarPubMed
Rutala, W. A., Katz, E. B. S., Sherertz, R. J. & Sarttbbi Jr, F. A. (1983). Environmental study of a methicillin-resistant Staphylococcus aureus epidemic in a burn unit. Journal of Clinical Microbiology 18, 683–688.Google Scholar
Townsend, D. E., Bolton, S., Ashdown, N. & Grubb, W. B. (1985). Transfer of plasmid-borne aminoglycoside-resistance determinants in Staphylococci. Journal of Medical Microbiology 20, 169–185.CrossRefGoogle ScholarPubMed
Wilson, C. R., Totten, P. A. & Baldwin, J. N. (1978). Rapid procedure for the detection of plasmids in Staphylococcus epidermidis. Applied and Environmental Microbiology 36, 368–374.CrossRefGoogle ScholarPubMed