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Microneutralization tests for serological typing and subtyping of foot-and-mouth disease virus strains

Published online by Cambridge University Press:  15 May 2009

M. M. Rweyemamu ,
J. C. Booth ,
Morwen Head  and
T. W. F. Pay
M. M. Rweyemamu
Affiliation:
The Wellcome Foundation Limited, Wellcome Foot-and-Mouth Disease Vaccine Leboratory, Pirbright, Woking, Surrey 0U24 ONQ, U.K.
J. C. Booth
Affiliation:
The Wellcome Foundation Limited, Wellcome Foot-and-Mouth Disease Vaccine Leboratory, Pirbright, Woking, Surrey 0U24 ONQ, U.K.
Morwen Head
Affiliation:
The Wellcome Foundation Limited, Wellcome Foot-and-Mouth Disease Vaccine Leboratory, Pirbright, Woking, Surrey 0U24 ONQ, U.K.
T. W. F. Pay
Affiliation:
The Wellcome Foundation Limited, Wellcome Foot-and-Mouth Disease Vaccine Leboratory, Pirbright, Woking, Surrey 0U24 ONQ, U.K.
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Summary

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A microneutralization test for serotyping of FMD viruses is described. It is based on earlier observations by Booth, Rweyemamu & Pay (1978) that dose-response relationships in quantal microneutralizations often deviated from linearity. The typing test described therefore utilizes undiluted virus preparations. In about 90% of samples a positive typing was obtained in contrast with about 50% for the complement fixation test. The test was also found to be susceptible to minimal quantities of heterotypic viral contamination.

For strain differentiation the microneutralization test was carried out as a checkerboard test. When compared with the complement fixation test it was found to be more specific. The necessity to utilize virus-neutralization test systems for comparing (FMD) virus strains particularly for the purpose of vaccine selection is emphasized. The two dimensional microneutralization test has been applied to a study of comparing FMDV vaccine strains for Europe, South America, the Middle East and East Africa.

Type
Research Article
Information
Journal of Hygiene , Volume 81 , Issue 1 , August 1978 , pp. 107 - 123
Copyright
Copyright © Cambridge University Press 1978

References

REFERENCES

Booth, J. C, Rweyemamu, M. M. & Pay, T. W. F. (1978). Dose-response relationships in a microneutralization test for foot-and-mouth disease viruses. Journal of Hygiene 80, 31–42.CrossRefGoogle Scholar
Brooksby, J. B. (1952). The technique of complement fixation in foot-and-mouth disease research. Agricultural Research Council Report Series no. 12.Google Scholar
Brooksby, J. B. (1968). Variants and immunity: definitions for serological investigations. International Symposium on Foot-and Mouth Disease. Variants and Immunity.Lyon,1967. Symposium Series. Immunobiological Standardization 8, 1–10.Google Scholar
Brown, F. & Crick, J. (1958). Application of agar gel precipitin tests to the study of the virus of foot-and-mouth disease. Virology 5, 133–44.CrossRefGoogle Scholar
Brown, F. & Smale, C. J. (1970). Demonstration of three specific sites on the surface of foot- and-mouth disease virus by antibody complexing. Journal of General Virology 7, 115–27.CrossRefGoogle ScholarPubMed
Capstick, P. B., Sellers, R. F. & Stewart, D. L. (1959). The neutralisation of the virus of foot-and-mouth disease by immune serum. Archiv fur die gesamte Virusforschung 9, 606–20.CrossRefGoogle Scholar
Cottral, G. E. (1972). Foot-and-mouth disease virus neutralization test cross reactions: a comparison of cattle and guinea pig serums. Proceedings of the 76th Annual Meeting of U.S. Animal Health Association, Florida, pp. 194–207.Google Scholar
Cowan, K. M. (1973). Antibody response to viral antigens. Advances in Immunology 17, 195–253.CrossRefGoogle ScholarPubMed
Cowan, K. M. & Graves, J. H. (1966). A third antigenic component associated with foot-and-mouth disease infection. Virology 30, 528–40.CrossRefGoogle ScholarPubMed
Cowan, K. M. & Wagner, G. G. (1970). Immunochemical studies of foot-and-mouth disease. VIII. Detection and quantitation of antibodies by radial immunodiffusion. Journal of Immunology 105, 557–66.CrossRefGoogle Scholar
Davie, J. (1964). A complement fixation technique for the quantitative measurement of antigenic differences between strains of the virus of foot-and-mouth disease. Journal of Hygiene 62, 401–11.Google ScholarPubMed
Forman, A. J. (1974). A study of foot-and-mouth disease virus strains by complement fixation. I. A model for the fixation of complement by antigen/antibody mixtures. Journal of Hygiene 72, 397–405.CrossRefGoogle Scholar
Forman, A. J. (1975). A comparison of some immunological methods for the differentiation of strains of foot-and-mouth disease virus. Journal of Hygiene 74, 215–25.CrossRefGoogle ScholarPubMed
Graves, J. H. (1960 a). The differentiation of subtypes (variants) of foot-and-mouth disease virus by serologic methods. I. Complement fixation test. American Journal of Veterinary Research 21, 687–90.Google ScholarPubMed
Graves, J. H. (1960 b). The differentiation of subtypes (variants) of foot-and-mouth disease virus by serologic methods. II. Precipitin test in agar. American Journal of Veterinary Reseasch 21, 691–3.Google ScholarPubMed
Karber, G. (1931). Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Archiv für experimentelle Pathologic und Pharmakologie 162, 480–83.Google Scholar
Lobo, C. A., Cowan, K. M., Trautman, R. & Hanson, R. P. (1974). Differentiation of type A foot-and-mouth disease virus subtypes by double and radial immunodiffusion analysis. American Journal of Veterinary Research 35, 1121–6.Google ScholarPubMed
Lucan, F., Fedida, M. & Dannacher, G. (1964). Mesure de l'immunité antiaphteuse postvaccinale de boeuf au moyen d'une èpreuve do séro-neutralisation en culture de tissus. Bulletin de l'Académie Véterinaire de France 37, 175–80.CrossRefGoogle Scholar
Martin, W. B. & Chapman, W. G. (1961). The tissue culture colour test for assaying the virus and neutralizing antibody of foot-and-mouth disease virus and its application to the measurement of immunity in cattle. Research in Veterinary Science 2, 53–61.CrossRefGoogle Scholar
Martinsen, J. S. (1971). Two plaque size variants of foot-and-mouth disease virus differing in neutralization by guinea pig antisera. Research in Veterinary Science 12, 399–400.CrossRefGoogle ScholarPubMed
McVicar, J. M. & Sutmoller, P. (1974). Foot-and-mouth disease virus: Plaque reduction neutralization test. Archiv für die gesamte Virusforschung 44, 168–72.CrossRefGoogle Scholar
Pay, T. W. F. (1972). Some observations on the potency testing of foot-and-mouth disease vaccine. Bulletin Office International des Epizooties 79, 1175–87.Google Scholar
Pay, T. W. F. & Parker, M. J. (1977). Some statistical and experimental design problems in the assessment of FMD vaccine potency. International Symposium on Foot-and-mouth Disease.Lyon,1976.Developments in Biological Standardization 35, 369–83.Google Scholar
Pereira, H. G. (1977). Subtyping of foot-and-mouth disease virus. International Symposium on Foot-and-Mouth Disease. Lyon, 1976. Developments in Biological Standardization 35, 167–74.Google Scholar
Rowlands, D. J., Cartwright, B. & Brown, F. (1969). Evidence for an internal antigen in foot-and-mouth disease virus. Journal of General Virology 4, 479–87.CrossRefGoogle ScholarPubMed
Rweyemamu, M. M., Booth, J. C. & Pay, T. W. F. (1975). Neutralization kinetics studies with type SAT 2 foot-and-mouth disease virus strains. Proceedings of the 20th World Veterinary Congress, Thessaloniki, 1975, vol. 3, pp. 2523–4.Google Scholar
Rweyemamu, M. M., Booth, J. C. & Pay, T. W. F. (1977 a). Neutralization kinetics studies with type SAT 2 foot-and-mouth disease virus strains. I. Factors that influence the rate and pattern of neutralization. Journal of Hygiene 78, 99–110.CrossRefGoogle ScholarPubMed
Rweyemamu, M. M., Booth, J. C., Parry, N. & Pay, T. W. F. (1977 b). Neutralization kinetics studies with type SAT 2 foot-and-mouth disease virus strains. II. Antigenic differ. entiation of vaccine strains. Journal of Hygiene 78, 429–38.CrossRefGoogle Scholar
Rweyemamu, M. M., Pay, T. W. F. & Parker, M. J. (1977 c). Serological differentiation of foot-and-mouth disease virus strains in relation to selection of suitable vaccine viruses. International Symposium on Foot-and-Mouth Disease.Lyon,1976. Developments in Biological Standardization 35, 205–14.Google Scholar
Stellman, C., Bornarel, P., Lang, R. & Terre, J. (1968). Contrôle quantitatif du vaccin antiaphteux. Analyse statistique de la relation liant les titre d'anticorps neutralisants au pourcentage de protection bovine. Recueil de Médicine Véter'inaire de l'Ecole d'Alfort 144, 325–51.Google Scholar
Sugimura, T. & Eissner, G. (1976). Typing of foot-and-mouth disease virus by fluorescent antibody technique. National Institute of Animal Health Quarterly 16, 152–9.Google ScholarPubMed
Wild, T. F., Burroughs, J. N. & Brown, F. (1969). Surface structure of foot-and-mouth disease virus. Journal of General Virology 4, 313–20.CrossRefGoogle ScholarPubMed