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Published online by Cambridge University Press: 15 May 2009
1. The commonly occurring bacteria may be divided into four groups on the basis of their behaviour at various temperatures. These are (i) the Staphylococci, not growing below 10° C, (ii) most strains of B. coli, B. proteus and Micrococci ceasing growth in the range 5−0° C, (iii) some strains of B. proteus etc. capable of growth at 0° C, (iv) many strains of Achromobacter, Pseudo monas, and various yeasts growing rapidly at 0° C. (sometimes in 5 days), and down to about −5° C. on unfrozen media.
2. Bacterial growth on frozen media has not been observed below −3° C.
The author is indebted to Dr G. S. Graham-Smith, F.R.S., who read the manuscript, to Mr W. A. Bmpey for the supply of cultures, and to Mr J. Barlow for assistance in the experimental work.