Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-15T01:56:15.495Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular epidemiology of recent United Kingdom isolates of Neisseria meningitidis serogroup C

Published online by Cambridge University Press:  15 May 2009

D. E. Yakubu ,
F. J. R. Abadi  and
T. H. Pennington
D. E. Yakubu
Affiliation:
Department of Medical Microbiology, University of Aberdeen Medical School, Foresterhill, Aberdeen ABO 2ZD
F. J. R. Abadi
Affiliation:
Department of Medical Microbiology, University of Aberdeen Medical School, Foresterhill, Aberdeen ABO 2ZD
T. H. Pennington
Affiliation:
Department of Medical Microbiology, University of Aberdeen Medical School, Foresterhill, Aberdeen ABO 2ZD
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The genomes of 34 recent United Kingdom isolates of Neisseria meningitidis serogroup C were examined by restriction enzyme digestion and by conventional and pulsed-field gel electrophoresis (PFGE). Strains were assigned to groups on the basis of the Dice similarity coefficient; strains with values of >92% were considered to be clonally related. Twelve clones were identified by PFGE. Strains of two clonal groups predominated. Restriction endonuclease analyses using the ‘high frequency cleavage’ endonuclease Stu I and conventional electrophoresis gave 11 groups; in general it had lower resolving power than PFGE.

Type
Research Article
Information
Epidemiology & Infection , Volume 113 , Issue 1 , August 1994 , pp. 53 - 65
Copyright
Copyright © Cambridge University Press 1994

References

REFERENCES

1.Jones, DM, Kaczmarski, EB. Meningococcal infections in England and Wales. Commun Dis Rep Rev 1993; 3: R129–31.Google ScholarPubMed
2.Jones, DM, Kaczmarski, EB. Meningococcal infections in England and Wales: 1991. Commun Dis Rep Rev 1992; 2: R61–3.Google ScholarPubMed
3.Fallon, RJ. Meningococcal infections in Scotland 1992. Communicable Diseases and Environmental Health in Scotland Weekly Report 1993; 27: 6–11.Google Scholar
4.Caugant, DA, Mocca, LF, Frasch, CE. Frøholm, LO, Zollinger, WD, Selander, RK. Genetic structure of Neisseria meningitidis populations in relation to serogroup, serotype, and outer membrane protein pattern. J Bacteriol 1987; 169: 2781–92.CrossRefGoogle ScholarPubMed
5.Caugant, DA, Bol, P, Høiby, EA, Zanen, HC, Frøholm, LO. Clones of serogroup B Neisseria meningitidis causing systemic disease in The Netherlands. 1958–1986. J Infect Dis 1990; 162:867–74.CrossRefGoogle ScholarPubMed
6.Caugant, DA, Frøholm, LO, Brøvre, K et al. , Intercontinental spread of a genetically distinctive complex of clones of Neisseria meningitidis causing epidemic disease. Proc Xatl Acad Sci USA 1986; 83: 4927–31.CrossRefGoogle ScholarPubMed
7.Cuevas, LE, Hart, CA. Chemoprophylaxis of bacterial meningitis. J Antimicrob Chemother 1993; 31 Suppl B: 79–91.CrossRefGoogle ScholarPubMed
8.Bjorvatn, B, Lund, V. Kristiansen, B-E, Korsnes, L. Spanne, O, Lindqvist, B. Application of restriction endonuclease fingerprinting of chromosomal DNA of Neisseria meningitidis. J Clin Microbiol 1984; 19: 763–5.CrossRefGoogle ScholarPubMed
9.Olyhoek, T, Crowe, BA, Achtman, M. Clonal population structure of Neisseria meningitidis serogroup A isolated from epidemic and pandemics between 1915 and 1983. Review Infect Dis 1987; 9: 665–82.CrossRefGoogle ScholarPubMed
10.Ohyhoek, T, Crowe, BA, Wall, RA, Achtman, M. Comparison of clonal analysis and DNA restriction analysis for typing of Neisseria meningitidis. Microbial Pathogen 1988; 4: 45–51.Google Scholar
11.Jordens, JZ, Pennington, TH. Characterization of Neisseria meningitidis isolates by ribosomal RNA gene restriction patterns and restriction endonuclease digestion of chromosomal DNA. Epidemiol Infect 1991; 107: 253–62.CrossRefGoogle ScholarPubMed
12.Fox, AJ, Jones, DM, Gray, JJ, Caugant, DA, Saunders, NA. An epidemiologically valuable typing method for Neisseria meningitidis by analysis of restriction fragment length polymorphisms. J Med Microbiol 1991; 34: 265–70.CrossRefGoogle ScholarPubMed
13.Smith, CL, Econome, JG, Schutt, A, Klco, S. Cantor, CR. A physical map of the Escherichia coli K12 genome. Science 1987; 236: 1448–52.CrossRefGoogle ScholarPubMed
14.Conard, B, Cole, ST. Genomic organization of the anaerobic pathogen Clostridium perfringens. Proc Natl Acad Sci USA 1989; 86: 6676–80.CrossRefGoogle Scholar
15.McClelland, M, Jones, R. Patel, Y, Nelson, M. Restriction endonucleases for pulsed field mapping of bacterial genomes. Nucleic Acids Res 197; 15: 5985–6005.Google Scholar
16.Pattee, PA. Genetic and physical mapping of the chromosome of Staphylococcus aureus NCTC 8325 In: Drlica, K, Riley, M, eds. The bacterial chromosome. Washington. D.C.: American Society for Microbiology, 1990: 163–9.Google Scholar
17.Arbeit, RD, Arthur, M, Dunn, R, Kim, C. Selander, RK, Goldstein, R. Resolution of recent evolutionary divergence among Escherichia coli from related lineages: the application of pulsed field electrophoresis to molecular epidemiology. J Infect Dis 1990; 161: 230–5.CrossRefGoogle ScholarPubMed
18.Prevost, G, Pottechar, B, Dahlet, M, Bient, M, Mantz, JM. Piemont, Y. Pulsed field gel electrophoresis as a new epidemiological tool for monitoring methicillin-resistant Staphylococcus aureus in an intensive care unit. J Hosp Infect 1991: 17: 255–69.CrossRefGoogle Scholar
19.Poh, CL. Lau, QC. Subtyping of Neisseria gonorrhoea auxotype-serovar groups by pulsed-field gel electrophoresis. J Med Microbiol 1993; 38: 366–70.CrossRefGoogle ScholarPubMed
20.Bygraves, JA. Maiden, MCJ. Analysis of the elonal relationships between strains of Neisseria meningitidis by pulsed field gel electrophoresis. J Gen Microbiol 1992; 138: 523–31.CrossRefGoogle Scholar
21.Pitcher, DG. Saunders, NA. Owen, RJ. Rapid extraction of bacterial genomic UNA with guanidium thiocyanate. Letts Appl Microbiol 1989; 8: 151–6.CrossRefGoogle Scholar
22.Dice, LR. Measure of the amount of ecologic associations between species. Ecology 1945; 26: 277–302.CrossRefGoogle Scholar
23.Sneath, PHA. Sokal, RR. Numerical taxonomy: The principles and practice of numerical classification. San Francisco: WH Freeman & Co. 1973.Google Scholar
24.Bautsch, W. A Nhe I macrorestriction map of the Neisseria meningitidis B1940 genome. FEMS Microbiol Lett 1993: 107: 191–8.CrossRefGoogle Scholar
25.Hart, CA. Rogers, TRF. eds. Meningococcal disease. J Med Microbiol 1993; 39: 3–25.CrossRefGoogle ScholarPubMed
26.Wang, J-F. Caugant, DA. Morelli, G, Kaumare, B. Achtman, M. Antigenic and epidemiologic properties of the ET-37 complex of Neisseria meningitidis. J Infect Dis 1993; 167: 1320–9.CrossRefGoogle ScholarPubMed
27.Pinner, RW. Gellin, BG. Bibb, WF et al. Meningococcal disease in the United States – 1986. J Infect Dis 1991; 164: 368–74.CrossRefGoogle ScholarPubMed
28.Mastrantonio, P, Congru, ME, Selander, RK, Caugant, DA. Genetic relationships among strains of Neisseria meningitidis causing disease in Italy. 1984–87. Epidemiol Infect 1991; 106: 143–50.CrossRefGoogle Scholar
29.Ashton, FE. Ryan, JA. Borczyk, A. Caugant, DA. Mancino, L. Huang, D. Emergence of a virulent clone of Neisseria meningitidis serotype 2a that is associated with meningococcal group C disease in Canada. J Clin Microbiol 1991; 29: 2489–93.CrossRefGoogle Scholar
30.Mulligan, ME. Arbeit, RD. Epidemiologic and clinical utility of typing systems for differentiating among strains of methieillin-resistant Staphylococcus aureus. Infect Control Hosp Epidemiol 1991: 12: 20–8.CrossRefGoogle ScholarPubMed
31.Caugant, DA, Zollinger, WD. Mocca, LF et al. , Genetic relationships and elonal population and elonal population structure of serotype 2 strains of Neisseria meningitidis. Infect Immun 1987; 55: 1503–13.CrossRefGoogle Scholar
32.Swaminathan, B. Matar, GM. Molecular typing methods. In: Persing, DH, Smith, TF, Tenover, FC. White, TJ. ed. Diagnostic molecular microbiology: Principles and applications. Washington. D.C.: American Society for Microbiology. 1993: 26–50.Google Scholar
33.Woods, TC. Helsel, LO. Swaminathan, B et al. Characterization of Neisseria meningitidis serogroup C by multilocus enzyme electrophoresis and ribosomal DNA restriction profiles (ributyping). J Clin Microbiol 1992; 30: 132–7.CrossRefGoogle Scholar