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The mycoplasmacidal properties of sodium hypochlorite

Published online by Cambridge University Press:  19 October 2009

D. H. Lee
Affiliation:
Biological Sciences Group, Chelsea College, Hortensia Road, London, SW1O 0QX
R. J. Miles
Affiliation:
Biological Sciences Group, Chelsea College, Hortensia Road, London, SW1O 0QX
B. F. Perry
Affiliation:
Research and Development Laboratories, Europe Africa Division, Richardson-Vicks Limited, Rusham Park, Whitehall Lane, Egham, Surrey, TW20 9NW, U.K.
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Summary

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The effect of hypochlorite concentration on Mycoplasma mycoides ssp. mycoides viability was tested under a variety of conditions. The experimental variables employed included chlorine-cell contact time, chlorine concentration, carrier system and organic loading. Initial populations of 106 c.f.u./ml were killed (no survivors in 1 ml) by hypoehlorite solution containing 25 p.p.m. available chlorine in 15 s in the absence of organic load and 50 p.p.m. available chlorine in 5 min in the presence of 1% protein. Higher concentrations of hypochlorite were required to disinfect a porous carrier system in the absence or presence of protein. The results are in contrast to previous reports that M. bovis is killed only by high hypochlorito concentrations.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1985

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