Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-14T04:32:10.878Z Has data issue: false hasContentIssue false
  • English
  • Français

Neutralization of TRIC organisms by antibody: enhancement by antisera prepared against immunoglobulins

Published online by Cambridge University Press:  15 May 2009

W. A. Blyth  and
Janice Taverne
W. A. Blyth
Affiliation:
MRC Trachoma Unit, Lister Institute of Preventive Medicine, Chelsea Bridge Road, London SW1W 8RH
Janice Taverne
Affiliation:
MRC Trachoma Unit, Lister Institute of Preventive Medicine, Chelsea Bridge Road, London SW1W 8RH
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The neutralizing activities of fowl, rabbit and mouse antisera prepared against TRIC agents were enhanced, in some cases over a 100-fold, by the addition of antiserum against the appropriate IgG. Significant non-specific inactivation of the organisms was observed in the absence of antiserum against TRIC agents, in reaction mixtures containing normal serum and antiserum against IgG. Since the extent of this inactivation showed a prozone, control tests with dilutions of normal serum equivalent to the full range of dilutions of the antiserum tested are necessary to show that enhancement of neutralization is specific.

Type
Research Article
Information
Journal of Hygiene , Volume 72 , Issue 1 , February 1974 , pp. 129 - 134
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Blyth, W. A. & Taverne, J. (1974). Cultivation of TRIC agents: a comparison between the use of BHK-21 and irradiated McCoy cells. Journal of Hygiene 72, 121.CrossRefGoogle ScholarPubMed
Brown, A., Elsner, V., Zebovitz, E. & Allen, W. P. (1969). Use of an antiglobulin plaque neutralization test among group A arboviruses. Proceedings of the Society for Experimental Biology and Medicine 130, 718–22.CrossRefGoogle ScholarPubMed
Collier, L. H. & Blyth, W. A. (1966). Immunogenicity of experimental trachoma vaccines in baboons. I. Experimental methods and preliminary tests with vaccines prepared in chick embryos and HeLa cells. Journal of Hygiene 64, 513–28.Google ScholarPubMed
Jones, B. R. (1961). Trachoma and allied infections. Transactions of the Ophthalmological Society of the United Kingdom 81, 367–78.Google ScholarPubMed
Lawn, A. M., Blyth, W. A. & Taverne, J. (1973). Interactions of TRIC organisms with macrophages and BHK-21 cells observed by electron microscopy. Journal of Hygiene 71, 515–28.CrossRefGoogle ScholarPubMed
T'ang, F. F., Chang, H. L., Huang, Y. T. & Wang, K. C. (1957). Studies on the etiology of trachoma with special reference to isolation of the virus in chick embryo. Chinese Medical Journal 75, 429–47.Google Scholar
Taverne, J. & Blyth, W. A. (1971). Interactions between trachoma organisms and macrophages. In Trachoma and Related Disorders (ed. Nichols, R. L., pp. 88–107). Proceedings of a symposium held in Boston Mass., 08 1970. Amsterdam: Excerpta Medica.Google Scholar
Taverne, J., Blyth, W. A. & Reeve, P. (1964). Toxicity of the agents of trachoma and inclusion conjunctivitis. Journal of general Microbiology 37, 271–5.CrossRefGoogle ScholarPubMed
Williams, T. D. & Hahon, N. (1970). Serum neutralization anti-IgG test for psittacosis. Infection and Immunity 2, 7–10.CrossRefGoogle ScholarPubMed