Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-15T01:25:51.608Z Has data issue: false hasContentIssue false
  • English
  • Français

A nucleotide sequence comparison of coxsackievirus B4 isolates from aquatic samples and clinical specimens

Published online by Cambridge University Press:  19 October 2009

M. S. Hughes ,
E. M. Hoey  and
P. V. Coyle
M. S. Hughes*
Affiliation:
Department of Microbiology & Immunobiology, Queen's University of Belfast, Royal Victoria Hospital, Belfast 12, N. Ireland
E. M. Hoey
Affiliation:
School of Biology and Biochemistry, Queen's University of Belfast, Medical Biology Centre, Belfast 9, N. Ireland
P. V. Coyle
Affiliation:
Regional Virus Laboratory, Royal Victoria Hospital, Belfast 12, N. Ireland
*
*Dr M. S. Hughes, Bacteriology Department, Veterinary Sciences Division, Stoney Road, Belfast BT4 3SD, N. Ireland.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Ten coxsackievirus B4 (CVB4) strains isolated from clinical and environmental sources in Northern Ireland in 1985–7, were compared at the nucleotide sequence level. Dideoxynucleotide sequencing of a polymerase chain reaction (PCR) amplified fragment, spanning the VP1/P2A genomic region, classified the isolates into two distinct groups or genotypes as defined by Rico-Hesse and colleagues for poliovirus type 1. Isolates within each group shared approximately 99% sequence identity at the nucleotide level whereas ≤86% sequence identity was shared between groups. One isolate derived from a clinical specimen in 1987 was grouped with six CVB4 isolates recovered from the aquatic environment in 1986–7. The second group comprised CVB4 isolates from clinical specimens in 1985–6. Both groups were different at the nucleotide level from the prototype strain isolated in 1950. It was concluded that the method could be used to sub-type CVB4 isolates and would be of value in epidemiological studies of CVB4. Predicted amino acid sequences revealed non-conservation of the tyrosine residue at the VP1/P2A cleavage site but were of little value in distinguishing CVB4 variants.

Type
Research Article
Information
Epidemiology & Infection , Volume 110 , Issue 2 , April 1993 , pp. 389 - 398
Copyright
Copyright © Cambridge University Press 1993

References

REFERENCES

1.Grist, N, Bell, EJ, Assaad, F.Enteroviruses in human disease. Prog Med Virol 1978; 24: 114–57.Google ScholarPubMed
2.Mahy, BWJ. Classification and general properties. In: Bendinelli, M, Friedman, H. eds. Coxsackieviruses; A general update. New York: Plenum Press. 1988; 1–13.Google Scholar
3.Hughes, MS, Coyle, PV, Connolly, JH. Enteroviruses in recreational waters of Northern Ireland. Epidemiol Infect 1992; 108: 529–36.CrossRefGoogle ScholarPubMed
4.Domingo, E, Martinez-Salas, E, Sobrino, F et al. . The quasispeeies (extremely heterogeneous) nature of viral RNA genome populations: biological relevance a review. Gene 1985: 40: 1–8.CrossRefGoogle ScholarPubMed
5.Prabhakar, BS, Haspel, MV, McClintock, PR, Notkins, AL. High frequency of antigenic variants among naturally occurring human coxsackie B4 virus isolates identified by monoclonal antibodies. Nature 1982; 300: 374–6.CrossRefGoogle ScholarPubMed
6.Yoon, JW. Austin, M, Onodera, T, Notkins, AL. Virus-induced diabetes mellitus. Isolation of a virus from the pancreas of a child with diabetic ketoacidosis. N Eng J Med 1979: 300: 1173–9.CrossRefGoogle ScholarPubMed
7.Chatterjee, NK, Haley, TM, Nejman, C. Functional alterations in pancreatic beta cells as a factor in virus-induced hvperglycaemia in mice. J Biol Chem 1985; 260: 12786–91.CrossRefGoogle ScholarPubMed
8.Gauntt, CJ, Trousdale, MD.LaBadie, DRL.Paque, RE.Nealon, T.Properties of coxsackievirus B3 variants which are amyocarditic or myocarditie for mice. J Med Virol 1979; 3: 207–20.CrossRefGoogle ScholarPubMed
9.Rico-Hesse, R, Pallanseh, MA.Nottay, BK, Kew, OM. Geographic distribution of wild poliovirus type 1 genotypes. Virology 1987: 160: 311–22.CrossRefGoogle ScholarPubMed
10.Iizuka, N, Kuge, S, Nomoto, A. Complete nucleotide sequence of the genome of coxsackievirus B1. J Virol 1987; 156: 64–73.CrossRefGoogle ScholarPubMed
11.Lindberg, AM, Stalhandske, POK, Pettersson, U. Genome of coxsackievirus B3. Virology 1987; 156: 50–63.CrossRefGoogle ScholarPubMed
12.Jenkins, O.Booth, JD.Minor, PD.Almond, JW.The complete nucleotide sequence of coxsackievirus B4 and its comparison to other members of the picomaviridae. J Gen Virol 1987; 68: 1835–48.CrossRefGoogle Scholar
13.Barron, AL, Olshevsky, C, Cohen, MM.Characteristics of the BGM line of cells from African Green Monkey kidney. Archiv Virusforsh 1970; 32: 389 92.CrossRefGoogle ScholarPubMed
14.Maniatis, T, Fritseh, EF, Sambrook, J.Molecular cloning: a laboratory manual. Cold Spring Harbor. NY: Cold Spring Harbor Laboratory 1982: 4.29 4.30.Google Scholar
15.Sanger, F, Nicklen, S, Coulson, AR. DNA sequencing with chain-terminating inhibitors. Proc Nat Acad Sci USA 1977; 74: 5463–7.CrossRefGoogle ScholarPubMed
16.Strikas, RA, Anderson, LJ, Parker, RA.Temporal and geographic patterns of isolates of nonpolio enterovirus in the United States, 1970–1983. J Infect Dis 1986; 153: 346–51.CrossRefGoogle ScholarPubMed
17.Morris, R, Sharp, DN. Cytopathic enteric viruses in wastewater effluents and surface waters. In: Butler, M, Medlen, AR, Morris, R, eds. Viruses and disinfection of water and wastewater. Surrey: University of Surrey, 1982; 32–41.Google Scholar
18.Zavate, O, Cotor, F, Avram, G, Ivan, A, Nastase, M. Surveillance of enterovirus circulation in children communities. Rev Roum Med-Virol 1986; 37: 61–6.Google ScholarPubMed
19.Rao, VC, Melnick, JL. Environmental virology. Wokingham: Van Nostrand Reinhold (UK) Co Ltd, 1986: 1.CrossRefGoogle Scholar