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Nucleotide substitutions in vanC-2 gene of Enterococcus casseliflavus isolates obtained from chickens

Published online by Cambridge University Press:  09 October 2002

T. MURASE ,
Y. MITO ,
K. OTSUKI ,
R. SUZUKI  and
S. YAMAI
T. MURASE
Affiliation:
Department of Veterinary Microbiology, Faculty of Agriculture, Tottori University, 4-101 Koyama, Tottori 680-8553, Japan
Y. MITO
Affiliation:
Department of Veterinary Microbiology, Faculty of Agriculture, Tottori University, 4-101 Koyama, Tottori 680-8553, Japan
K. OTSUKI
Affiliation:
Department of Veterinary Microbiology, Faculty of Agriculture, Tottori University, 4-101 Koyama, Tottori 680-8553, Japan
R. SUZUKI
Affiliation:
Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory, Yokohama 241-0815, Japan
S. YAMAI
Affiliation:
Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory, Yokohama 241-0815, Japan
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Abstract

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DNA sequencing of the vanC-2 gene was partially carried out on 10 isolates of Enterococcus casseliflavus obtained from 8 samples of imported chickens in Japan between July 1999 and June 2001 to evaluate the variation in the gene. Forty nucleotide substitutions in 36 codons were identified within 345 base pairs when compared with the vanC-2 sequence of the reference strain E. casseliflavus ATCC25788. Identical nucleotide substitutions were commonly found in the isolates recovered from chickens imported from both Brazil and China. Pulsed-field gel electrophoresis (PFGE) patterns of NotI-digested chromosomal DNA of these strains were distinguished by two, or more than six, band differences. These observations suggest that sequencing of the vanC-2 gene may be helpful for epidemiological investigation in combination with the PFGE analyses of the isolates, although particular genotypes are unlikely to be restricted to each of the countries that exported chickens.

Type
Short Report
Information
Epidemiology & Infection , Volume 129 , Issue 2 , October 2002 , pp. 421 - 424
Copyright
© 2002 Cambridge University Press