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Observations on the viability of Trichomonas foetus during the process of freezing to –79° C. and thawing in the presence of glycerol

Published online by Cambridge University Press:  15 May 2009

L. P. Joyner  and
G. H. Bennett
L. P. Joyner
Affiliation:
Ministry of Agriculture, Fisheries and Food, Veterinary Laboratory, Weybridge
G. H. Bennett
Affiliation:
Ministry of Agriculture, Fisheries and Food, Veterinary Laboratory, Weybridge
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1. It has been confirmed that Trichomonas foetus fails to survive freezing and thawing in the presence of 10 % glycerol in egg-yolk-citrate diluent. Under the same conditions adequate sperm survival was demonstrated.

2. Trichomonads were particularly sensitive to the toxic effects of glycerol when suspended in egg-yolk citrate.

3. Trichomonads will survive freezing and thawing when suspended in other diluents such as egg-yolk phosphate or milk.

Type
Research Article
Information
Journal of Hygiene , Volume 54 , Issue 3 , September 1956 , pp. 335 - 341
Copyright
Copyright © Cambridge University Press 1956

References

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