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Occurrence of shigatoxinogenic Escherichia coli O157 in Norwegian cattle herds

Published online by Cambridge University Press:  01 February 1998

L. VOLD ,
B. KLUNGSETH JOHANSEN ,
H. KRUSE ,
E. SKJERVE  and
Y. WASTESON
L. VOLD
Affiliation:
Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of Veterinary Medicine, P.O. Box 8146. Dep., 0033 Oslo, Norway
B. KLUNGSETH JOHANSEN
Affiliation:
Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of Veterinary Medicine, P.O. Box 8146. Dep., 0033 Oslo, Norway
H. KRUSE
Affiliation:
Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of Veterinary Medicine, P.O. Box 8146. Dep., 0033 Oslo, Norway
E. SKJERVE
Affiliation:
Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of Veterinary Medicine, P.O. Box 8146. Dep., 0033 Oslo, Norway
Y. WASTESON
Affiliation:
Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of Veterinary Medicine, P.O. Box 8146. Dep., 0033 Oslo, Norway
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Abstract

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To investigate if there is a reservoir of Escherichia coli O157 in Norwegian cattle, faecal samples from 197 cattle herds were screened for E. coli O157 by the use of immunomagnetic separation (IMS) and PCR during the 1995 grazing season. Six E. coli O157[ratio ]H-isolates were detected in two herds, one isolate in one and five in the other. The isolates carried the stx1, stx2, and eae genes, and a 90 MDa virulence plasmid. They were toxinogenic in a Vero cell assay. From 57 other herds, 137 faecal samples were positive for stx1 and/or stx2 genes detected by PCR run directly on IMS-isolated material. Among these samples, stx2 were the most widely distributed toxin encoding genes. No difference was found among milking cows and heifers in the rate of stx1 and/or stx2 in positive samples.

Type
Research Article
Information
Epidemiology & Infection , Volume 120 , Issue 1 , February 1998 , pp. 21 - 28
Copyright
© 1998 Cambridge University Press