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PCR-based characterization of Yersinia enterocolitica: comparison with biotyping and serotyping

Published online by Cambridge University Press:  15 May 2009

P. T. Odinot ,
J. F. G. M. Meis ,
P. J. J. C. Van Den Hurk ,
J. A. A. Hoogkamp-Korstanje  and
W. J. G. Melchers
P. T. Odinot
Affiliation:
University Hospital Nijmegen, Department of Medical Microbiology, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
J. F. G. M. Meis
Affiliation:
University Hospital Nijmegen, Department of Medical Microbiology, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
P. J. J. C. Van Den Hurk
Affiliation:
University Hospital Nijmegen, Department of Medical Microbiology, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
J. A. A. Hoogkamp-Korstanje
Affiliation:
University Hospital Nijmegen, Department of Medical Microbiology, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
W. J. G. Melchers
Affiliation:
University Hospital Nijmegen, Department of Medical Microbiology, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
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Summary

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PCR-based DNA fingerprinting was used to characterize 48 clinical isolates of Yersinia enterocolitica. The samples were examined by random amplified polymorphic DNA (RAPD-PCR) and inter-repeat PCR (IR-PCR). IR-PCR with two enterobacterial repetitive intergenic consensus primers resulted in patterns which were poorly discriminated; 2 of 11 arbitrary primers (RAPD-PCR) provided sufficient discriminatory power. In comparisons with serotyping and biotyping, RAPD-fingerprinting was the most discriminatory technique and may therefore be a valuable epidemiological tool for the study of Y. enterocolitica infections.

Type
Research Article
Information
Epidemiology & Infection , Volume 115 , Issue 2 , October 1995 , pp. 269 - 277
Copyright
Copyright © Cambridge University Press 1995

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