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The production of monoclonal antibodies to rubella haemagglutinin and their use in antibody-capture assays for rubella-specific IgM

Published online by Cambridge University Press:  25 March 2010

R. S. Tedder ,
J. L. Yao  and
M. J. Anderson
R. S. Tedder
Affiliation:
Department of Virology, Middlesex Hospital Medical School, Riding House Street, London W1P 7PP
J. L. Yao
Affiliation:
Department of Virology, Middlesex Hospital Medical School, Riding House Street, London W1P 7PP
M. J. Anderson
Affiliation:
Department of Medical Microbiology, King's College Hospital Medical School, Denmark Hill, London SE5 8RX
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Mice were immunized by three intraperitoneal and one intravenous injection of rubella haemagglutinin. Splenocytes from these mice were fused with the cells of a syngeneic myeloma cell line, and following culture for various periods of time, single-cell clones were derived by the technique of limiting dilution.

A total of 139 clones were derived from 13 parent hybrid cultures. To date, four of these cloned cultures have been propagated as ascitic tumours in mice. The preparation of IgG from ascitic fluid and labelling of this antibody with 125I is described. Results indicate that the use of labelled monoclonal antibodies as indicator reagents in solid-phase IgM antibody capture assays for the detection of rubella-specific IgM results in enhanced performance of these tests.

Type
Research Article
Information
Journal of Hygiene , Volume 88 , Issue 2 , April 1982 , pp. 335 - 350
Copyright
Copyright © Cambridge University Press 1982

References

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