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Properties of a cell-wall-defective variant of Brucella abortus of bovine origin

Published online by Cambridge University Press:  15 May 2009

M. J. Corbel ,
A. C. Scott  and
H. M. Ross
M. J. Corbel
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, New Haw, Weybridge, Surrey
A. C. Scott
Affiliation:
Ministry of Agriculture, Fisheries and Food, Central Veterinary Laboratory, New Haw, Weybridge, Surrey
H. M. Ross
Affiliation:
The North of Scotland College of Agriculture, Veterinary Investigation Centre, Drummondhill, Stratherrick Road, Inverness, IV2 4JZ
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The properties of an atypical Brucella strain isolated from lymph node tissue of a cow slaughtered as a brucellosis reactor were examined. The organism was Gram negative and highly pleomorphic, existing as cocci, coccobacilli, rods, branched and irregular forms which stained with fluorescent antibody conjugates prepared against rough and smooth Brucella abortus strains. It produced lecithinase and required at least 15% v/v equine or bovine serum for growth. It did not need supplementary CO2 for growth, produced H2S and was inhibited by brucella dyes and partially by i-erythritol. Growth inhibition or lysis was produced by brucellaphages. The organism was not pathogenic for guinea-pigs or mice but evoked antibodies mainly to rough Brucella antigens.

Type
Research Article
Information
Journal of Hygiene , Volume 85 , Issue 1 , August 1980 , pp. 103 - 113
Copyright
Copyright © Cambridge University Press 1980

References

Corbel, M. J. (1973). The direct fluorescent antibody test for detection of Brucella abortus in bovine abortion material. Journal of Hygiene 71, 123.Google Scholar
Corbel, M. J. (1975). The serological relationship between Brucella spp., Yersinia entero-colitica serotype IX and Salmonella serotypes of Kauffmann—White group N. Journal of Hygiene 75, 151.Google Scholar
Corbel, M. J. (1977). Production of a phage variant lytic for non-smooth Brucella strains. Annali Sclavo 19, 99.Google ScholarPubMed
Corbel, M. J. & Bracewell, C. D. (1976). The serological response to rough and smooth brucella antigens in cattle vaccinated with Brucella abortus strain 45/20 adjuvant vaccine. Developments in Biological Standardization 31, 351.Google ScholarPubMed
Corbel, M. J., Gill, K. P. W. & Thomas, E. L. (1978). Methods for the identification of Brucella. Ministry of Agriculture, Fisheries and Food, Pinner Middlesex.Google Scholar
Douglas, J. T. & Elberg, S. S. (1976). Isolation of Brucella melitensis phage of broad biotype and species specificity. Infection and Immunity 14, 306.CrossRefGoogle ScholarPubMed
Farrell, I. D. (1974). The development of a new selective medium for the isolation of Brucella abortus from contaminated sources. Research in Veterinary Science 16, 280.CrossRefGoogle ScholarPubMed
Gatt, S. (1973). In Metabolic Inhibitors (ed. Hochester, R. M., Quastel, G. H. and Kates, M.), pp. 349–87. New York: Academic Press.CrossRefGoogle Scholar
Hatten, B. A. (1973). Growth characteristics of microorganisms occurring in penicillin-treated Brucella abortus cultures. Proceedings of the Society for Experimental Biology and Medicine, New York 142, 909.CrossRefGoogle ScholarPubMed
Hines, W. D., Freeman, B. A. & Pearson, G. R. (1964 a). Production and characterization of Brucella spheroplasts. Journal of Bacteriology 87, 438.CrossRefGoogle ScholarPubMed
Hines, W. D., Freeman, B. A. & Pearson, G. R. (1964 b). Fine structure of Brucella suis spheroplasts. Journal of Bacteriology 87, 1492.CrossRefGoogle ScholarPubMed
Madoff, S. (1960). Isolation and identification of PPLO. Annals of the New York Academy of Sciences 79, 383.CrossRefGoogle ScholarPubMed
Mattman, L. H. (1974). In Cell Wall Deficient forms. Cleveland, Ohio: CRC Press Inc.Google Scholar
McGhee, J. R. & Freeman, B. A. (1970). Osmotically sensitive Brucella in infected and normal macrophages. Infection and Immunity 1, 146.CrossRefGoogle Scholar
Meyer, M. E. (1976). Evolution and taxonomy in the genus Brucella: steroid hormone induction of filterable forms with altered characteristics after reversion. American Journal of Veterinary Research 37, 207.Google Scholar
Morgan, W. J. B. (1963). The examination of Brucella cultures for lysis by phage. Journal of General Microbiology 30, 437.Google Scholar
Morris, J. A. & Corbel, M. J. (1973). Properties of a new phage lytic for Brucella suis. Journal of General Virology 21, 539.CrossRefGoogle ScholarPubMed
Nelson, E. L. & Pickett, M. J. (1951). The recovery of L forms of Brucella and their relation to Brucella phage. Journal of Infectious Diseases 89, 226.Google Scholar
Peschkov, J. & Feodorov, V. (1978). Comparative studies on the ultrastructure of L forms obtained from S and R variants of Brucella suis 1330. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene Abteilung 1/Originale A 240, 94.Google Scholar
Roux, J. & Sassine, J. (1971). Étude d'une souche fixée de sphéroplastes (formes L) de Brucella melitensis. Annales de l'Institut Pasteur 120, 174.Google Scholar
Thorns, C. J. (1978). Identification of the products of the ‘Film and Spots’ reaction of Mycoplasma bovis and Mycoplasma bovigenitalium. Journal of Biological Standardization 6, 127.CrossRefGoogle ScholarPubMed