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Prospective study of verocytotoxin–producing, enteroaggregative and diffusely adherent Escherichia coli in different diarrhoeal states

Published online by Cambridge University Press:  15 May 2009

M. G. Brook ,
H. R. Smith ,
B. A. Bannister ,
M. McConnell ,
H. Chart ,
S. M. Scotland ,
A. Sawyer ,
M. Smith  and
B. Rowe
M. G. Brook*
Affiliation:
Departments of Infectious Diseases NHS Trust, London
H. R. Smith
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, Public Health Laboratory Service, Colindale, London
B. A. Bannister
Affiliation:
Departments of Infectious Diseases NHS Trust, London
M. McConnell
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, Public Health Laboratory Service, Colindale, London
H. Chart
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, Public Health Laboratory Service, Colindale, London
S. M. Scotland
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, Public Health Laboratory Service, Colindale, London
A. Sawyer
Affiliation:
Gastroenterology, Royal Free Hospital NHS Trust, London
M. Smith
Affiliation:
Gastroenterology, Royal Free Hospital NHS Trust, London
B. Rowe
Affiliation:
Laboratory of Enteric Pathogens, Central Public Health Laboratory, Public Health Laboratory Service, Colindale, London
*
*Correspondence and reprint requests: Department of Infectious and Tropical Diseases, Coppetts Wood Unit, Royal Free Hospital NHS Trust, Coppetts Road, London N10 1JN.
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One hundred and eighty–one stool specimens from patients with various types of diarrhoea (135 patients) or from non-diarrhoeal controls (23 acute medical patients, 23 inflammatory bowel disease in remission) were investigated using a colony–blot DNA hybridization assay for the presence of Verocytotoxin–producing (VTEC), enteroaggregative (EAggEC) and diffusely adherent (DAEC) Escherichia coli. Twelve patients had probe–positive EAggEC in the stool and 8 of these had diarrhoea, 6 following recent travel. Eight patients had DAEC, 7 of whom had travellers diarrhoea. Six of 10 (60%) travellers with gastroenteritis, but without a recognized enteric pathogen, were positive for EAggEC (4) or DAEC (2). Five of 10 (50%) travellers with gastroenteritis related to a recognized enteric pathogen also had DAEC identified in their stool. Of the 23 acute medical control patients 11 had been abroad, 4 of these were immigrants and had EAggEC. VTEC were not found and, with one exception, immunoassays for antibodies to E. coli O 157 and O 2 lipopolysaccharides were negative.

Type
Research Article
Information
Epidemiology & Infection , Volume 112 , Issue 1 , February 1994 , pp. 63 - 67
Copyright
Copyright © Cambridge University Press 1994

References

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