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Pseudomonas aeruginosa cross-colonization and persistence in patients with cystic fibrosis. Use of a DNA probe

Published online by Cambridge University Press:  15 May 2009

Christiane Wolz ,
Gerd Kiosz ,
John W. Ogle ,
Michael L. Vasil ,
Urs Schaad ,
Konrad Botzenhart  and
Gerd Döring
Christiane Wolz
Affiliation:
Hygiene Institute, University of Tübingen, Tübingen, Federal Republic of Germany, Inselhospital, BernSwitzerland
Gerd Kiosz
Affiliation:
Kurklinik Satteldüne, Amrum, FRG, University of Colorado, Inselhospital, BernSwitzerland
John W. Ogle
Affiliation:
Pediatrics, Inselhospital, BernSwitzerland
Michael L. Vasil
Affiliation:
Departments of Microbiology and Immunology and Pediatrics, Inselhospital, BernSwitzerland
Urs Schaad
Affiliation:
University of Colorado, Denver, USA
Konrad Botzenhart
Affiliation:
Hygiene Institute, University of Tübingen, Tübingen, Federal Republic of Germany, Inselhospital, BernSwitzerland
Gerd Döring*
Affiliation:
Hygiene Institute, University of Tübingen, Tübingen, Federal Republic of Germany, Inselhospital, BernSwitzerland
*
1Professor Gerd Döring, Abt, Allgemeine Hygiene und Umwelthygiene, Hygiene-Institut. Universität Tübingen, Silcherstrasse 7, D-7400 Tübingen, FRG.
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To investigate cross-colonization with and persistence of Pseudomonas aeruginosa in cystic fibrosis (CF). 181 isolates from 76 CF patients were typed using a P. aeruginosa -specific DNA probe. Whereas sibling pairs predominantly harboured genotypically identical P. aeruginosa strains, all of the other patients harboured different strains. Seventy-nine per cent (22/31) of the infected CF patients harboured the same strains at the beginning and the end of a summer camp. A change of strains was seen in 10% (3/31) of the patients at the end of the camp. Forty-six per cent (6/13) of the patients who were apparently initially uninfected, acquired P. aeruginosa by the end of the period. Genotyping proved that strain change or acquisition was due to cross-colonization in four of nine cases. Very little P. aeruginosa was isolated from the inanimate environment. Persistence of P. aeruginosa after a temporary loss due to antibiotic therapy was seen in 12/16 paired patient strains before and after antibiotic therapy. Thus, suppression followed a flare-up seemed to occur in these patients rather than eradication and a new infection. When 35 patients were followed over a period of 6 months, 7 (20%) changed the strain in their sputum. Only one of 43 patients harboured two different P. aeruginosa strains simultaneously over a long period.

Type
Research Article
Information
Epidemiology & Infection , Volume 102 , Issue 2 , April 1989 , pp. 205 - 214
Copyright
Copyright © Cambridge University Press 1989

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