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Rapid field test for detection of hantavirus antibodies in rodents

Published online by Cambridge University Press:  12 May 2004

H. SIROLA ,
E. R. K. KALLIO ,
V. KOISTINEN ,
I. KURONEN ,
Å. LUNDKVIST ,
A. VAHERI ,
O. VAPALAHTI ,
H. HENTTONEN  and
A. NÄRVÄNEN
H. SIROLA
Affiliation:
Department of Chemistry, University of Kuopio, Kuopio, Finland
E. R. K. KALLIO
Affiliation:
Vantaa Research Centre, Finnish Forest Research Institute, Vantaa, Finland Department of Ecology and Systematic, University of Helsinki, Helsinki, Finland
V. KOISTINEN
Affiliation:
Department of Virology, Haartman Institute, University of Helsinki, Helsinki, Finland
I. KURONEN
Affiliation:
Oy Reagena Ltd, Toivala, Finland
Å. LUNDKVIST
Affiliation:
Swedish Institute for Infectious Disease Control, and Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden
A. VAHERI
Affiliation:
Department of Virology, Haartman Institute, University of Helsinki, Helsinki, Finland HUCH Laboratory Diagnostics, Helsinki, Finland
O. VAPALAHTI
Affiliation:
Department of Virology, Haartman Institute, University of Helsinki, Helsinki, Finland HUCH Laboratory Diagnostics, Helsinki, Finland Division of Microbiology and Epidemiology, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland
H. HENTTONEN
Affiliation:
Vantaa Research Centre, Finnish Forest Research Institute, Vantaa, Finland
A. NÄRVÄNEN
Affiliation:
Department of Chemistry, University of Kuopio, Kuopio, Finland
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Abstract

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Puumala virus (PUUV) is the causative agent of nephropathia epidemica, a mild form of haemorrhagic fever with renal syndrome. PUUV is transmitted to humans via aerosolized excreta of the infected bank vole (Clethrionomys glareolus). Current methods for screening of the PUUV prevalence among bank vole populations are laborious, combining sampling in the field and subsequent analyses in the laboratory. In order to facilitate animal testing, a new serological immunochromatographic rapid test was developed. The test uses PUUV nucleocapsid protein as antigen, and it detects anti-PUUV IgG antibodies in rodents. With fresh and undiluted bank-vole blood samples (n=105) the efficacy of the test was 100%, and with frozen and diluted samples (n=78) the efficacy was 91%. The test was also shown to detect related hantavirus infections in Norway lemmings and sibling voles (n=31) with 99% efficacy. The test provides an applicable tool for studying PUUV and related hantavirus infections in arvicoline rodents.

Type
Research Article
Information
Epidemiology & Infection , Volume 132 , Issue 3 , June 2004 , pp. 549 - 553
Copyright
© 2004 Cambridge University Press