Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-14T22:09:44.310Z Has data issue: false hasContentIssue false
  • English
  • Français

A rapid immunofluorescence technique for detecting salmonellae in raw meat

Published online by Cambridge University Press:  15 May 2009

D. L. Georgala  and
Margery Boothroyd
D. L. Georgala
Affiliation:
Unilever Research Laboratory, Colworth House, Sharnbrook, Bedford
Margery Boothroyd
Affiliation:
Unilever Research Laboratory, Colworth House, Sharnbrook, Bedford
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A rapid 18 hr. technique has been developed for detecting salmonella contaminated carcass and boneless meats. It is based on 43° C. selenite enrichment of samples, followed by immunofluorescent detection of salmonella cells in the enrichment. In tests with 286 meat samples the rapid and conventional techniques agreed in the detection of 93 positive and 149 negative samples. The two tests failed to agree for the remaining 44 samples. The rapid technique thus lacks precision, but could be used as a rapid ‘presumptive’ salmonella test, so that contaminated material could be prevented from reaching the processing lines of food factories.

We thank Dr Betty Hobbs of the Food Hygiene Laboratory for kindly sup plying the meat samples used in this investigation, Dr Patricia Bradstreet of the Standards Laboratory for Serological Reagents for the salmonella diagnostic antisera and Mr R. Kenworthy for performing the goat injections.

Type
Research Article
Information
Journal of Hygiene , Volume 62 , Issue 3 , September 1964 , pp. 319 - 327
Copyright
Copyright © Cambridge University Press 1964

References

REFERENCES

Arkhangel'skii, I. I. & Kartashova, V. M. (1962). Accelerated methods of detecting salmonella in milk. Veterinariya, 9, 74.Google Scholar
Beutner, E. H. (1961). Irnmunofluorescent staining: the fluorescent antibody method. Bact. Rev. 25, 49.CrossRefGoogle ScholarPubMed
Chadwick, C. S., McEntegart, M. G. & Nairn, R. C. (1958 a). Fluorescent protein tracing: a simple alternative to fluorescein. Lancet, i, 412.CrossRefGoogle Scholar
Chadwick, C. S., McEntegart, M. G. & Nairn, R. C. (1958 b). Fluorescent protein tracers: a trial of new fluorochromes and the development of an alternative to fluorescein. Immunology, 3, 363.Google Scholar
Christensen, W. B. (1946). Urea decomposition as a means of differentiating proteus and paracolon cultures from each other and from salmonella and shigella types. J. Bact. 52, 461.CrossRefGoogle ScholarPubMed
Galbrarith, N. S., Hobbs, B. C., Smith, M. E. & Tomlinson, A. J. H. (1960). Salmonellae in desiccated coconut. Mon. Bull. Minist. Hlth Lab. Serv. 19, 99.Google Scholar
Georgala, D. L. (1963). Methods for isolating salmonella from foods. Food Science and Technology, Vol. 2, 1st ed. New York: Gordon and Breach (in the Press).Google Scholar
Harvey, R. W. S. & Thomson, S. (1953). Optimum temperature of incubation for isolation of salmonellae. Mon. Bull. Minist. Hlth Lab. Serv. 12, 149.Google ScholarPubMed
Hobbs, B. C. (1962). Salmonellae. Chemical and Biological Hazards in Food, 1st ed., Ames, Iowa: Iowa State University Press.Google Scholar
Hobbs, B. C. & Wilson, J. C. (1959). Contamination of wholesale meat supplies with salmonellae and heat-resistant Clostridium welchii. Mon. Bull. Minist. Hlth Lab. Serv. 18, 198.Google ScholarPubMed
Jameson, J. E. (1961). A simple method for inducing motility and phase change in salmonella. Mon. Bull. Minist. Hlth Lab. Serv. 20, 14.Google Scholar
Kampelmacher, E. H. (1963). Public health and poultry products. Brit. vet. J. 119, 110.CrossRefGoogle Scholar
Leifson, E. (1936). New selenite enrichment media for the isolation of typhoid and paratyphoid (salmonella) bacilli. Amer. J. Hyg. 24, 423.Google Scholar
Le, Minor L. & Ben, Hamida F. (1962). Avantages de la recherche de la β-galactosidase sur celle de la fermentation du lactose en milieu complexe dans le diagnostic bacteriologique en particulier des Enterobacteriaceae. Ann. Inst. Pasteur, 102, 267.Google Scholar
Memorandum (1961). Serological reagents for bacteriological diagnosis. Mon. Bull. Minist. Hlth Lab. Serv. 20, 134.Google Scholar
Møller, V. (1955). Simplified tests for some amino acid decarboxylases and for the arginine dihydrolase system. Acta path. microbiol. scand. 36, 158.CrossRefGoogle ScholarPubMed
Nairn, R. C. (1962). Fluorescent Protein Tracing, 1st ed. Edinburgh: E. and S. Livingstone Ltd.Google Scholar
Report (1958). The contamination of egg products with salmonellae with particular reference to Salm. paratyphi B. Mon. Bull. Minist. Hlth Lab. Serv. 17, 36.Google Scholar
Riggs, J. L., Seiwald, R. J., Burckhalter, J. H., Downs, C. M. & Metcalf, T. G. (1958). Isothiocyanate compounds as fluorescent labelling agents for immune serum. Amer. J. Path. 34, 1081.Google ScholarPubMed
Thomason, B. M., Cherry, W. B. & Edwards, P. R. (1959). Staining bacterial smears with fluorescent antibody. VI. Identification of salmonellae in faecal smears. J. Bact. 77, 478.CrossRefGoogle Scholar
Thomason, B. M., Cherry, W. B. & Moody, M. D. (1957). Staining bacterial smears with fluorescent antibody. III. Antigenic analysis of Salmonella typhosa by means of fluorescent antibody and agglutination reactions. J. Beat. 74, 525.Google ScholarPubMed