Hostname: page-component-745bb68f8f-mzp66 Total loading time: 0 Render date: 2025-02-05T01:54:00.808Z Has data issue: false hasContentIssue false
  • English
  • Français

Reducing the endotoxic activity of pertussis vaccine

Published online by Cambridge University Press:  25 March 2010

R. M. Bannatyne  and
Rose Cheung
R. M. Bannatyne
Affiliation:
Department of Bacteriology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8
Rose Cheung
Affiliation:
Department of Bacteriology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Unadsorbed, regular production pertussis vaccine was treated with polymyxin B sulphate at concentrations of 25,50 and 100 μg/ml. The toxic activity of treated and untreated vaccines was compared using both the limulus amoebocyte lysate test and the mouse-weight-gain test. Protective efficacy was also assessed by the mouse protection test. No discernible effect on either toxicity or efficacy of the pertussis vaccine was observed. When the vaccine was treated with 5000 μg/ml of polymyxin, endotoxic activity assessed by the limulus lysate test appeared to be abolished.

Type
Research Article
Information
Journal of Hygiene , Volume 87 , Issue 3 , December 1981 , pp. 377 - 381
Copyright
Copyright © Cambridge University Press 1981

References

Bannatyne, R. M. & Cheung, R. (1979). Protective effect of polymyxin Bsulfate in experimental enterobaeterial infection in mice. Canadian Journal of Microbiology 25, 995–998CrossRefGoogle Scholar
Bannatyne, R. M., Harnett, N. M., Lee, K. & Biggar, W. D. (1977). Inhibition of the biologic effects of endotoxin on neutrophils by polymyxin B sulfate. Journal of Infectious Diseases 136, 469–474.CrossRefGoogle ScholarPubMed
Bannatyne, R. M., Harnett, N. M. & Cheung, R. (1977). Protective effect of polymyxin B sulfate in experimental meningococcal infection in mice. Canadian Journal of Microbiology 23, 1526–1528.CrossRefGoogle ScholarPubMed
Cooper, J. F. & Pearson, S. M. (1977). Detection of endotoxin in biological products by the limulus test. Developmental Biological Standardization 34, 7–13.Google ScholarPubMed
Cooperstock, M. S. (1974). Inactivation of endotoxin by polymyxin B. Antimicrobial Agents and Chemotherapy 6, 422–425.CrossRefGoogle ScholarPubMed
Corrigan, J. J. & Bell, B. M. (1971). Endotoxin-induced intravascular coagulation: Prevention with polymyxin B sulfate. Journal of Laboratory and Clinical Medicine 77, 802–810.Google ScholarPubMed
From, A. H. L., Good, R. A. & Fong, J. A. (1972). The effects of polymyxin B on lethality in canine endotoxin shock. Circulation Supplement ii, 153.Google Scholar
Geier, M. R., Stanbro, H. & Merrill, C. R. (1978). Endotoxins in commercial vaccines. Applied Microbiology 36, 445–449.Google ScholarPubMed
Ishida, S. (1968). Characterisation of the body weight-decreasing toxicities to mice by the lymphocytosis-promoting factor and the heat labile toxin of B. pertussis and endotoxin. Japanese Journal of Medical Science and Biology 21, 115–135.CrossRefGoogle ScholarPubMed
Knight, P. A. & Lucken, R. N. (1977). Studies on the toxicity of a variety of human vaccines as measured by laboratory tests. Developmental Biological Standardization 34, 181–187.Google ScholarPubMed
Koike, M., Iida, K. & Matsuo, T. (1969). Electron microscopic studies on mode of action of polymyxin. Journal of Bacteriology 97, 448–452.CrossRefGoogle ScholarPubMed
Kurokawa, M., Ishida, S., Iwasa, S.Asakawa, S. & Kuratsuka, K. (1968). Attempts at analysis of toxicity of pertussis vaccine. Japanese Journal of Medical Science and Biology 21, 137–153.CrossRefGoogle ScholarPubMed
Kurokawa, M., Ishida, S. & Asakawa, S. (1969). Attempts at analysis of toxicity of pertussis vaccine. Japanese Journal of Medical Science and Biology 22, 293–307.CrossRefGoogle ScholarPubMed
Kuronen, T., Peltola, H., Nors, T., Haque, N. & Makela, P. H. (1977). Adverse reactions and endotoxin content of polysaccharide vaccines. Developmental Biological Standardization 34, 181–187.Google ScholarPubMed
Lopes, J. & Inniss, W. E. (1969). Electronmicroscopy of effect of polymyxin on Escherichia coli lipopolysaccharide. Journal of Bacteriology 100, 1128–1130.CrossRefGoogle Scholar
Nagel, J. (1970). Investigations into the preparation of a non-toxic whole cell pertussis vaccine. Symposium Series on Immunogiological Standardization 13, 234–238.Google Scholar
Nagel, J. & De Graaf, S. (1978). Extraction and fractionation of Bordetella pertussis vaccine. International Symposium on Pertussis, Bethesda, Maryland, U.S.A. Nov. Pp. 327–330.Google Scholar
Nakase, Y. (1978). Toxicity and potency of a purified pertussis vaccine. International Symposium on Pertussis. Bethesda, Maryland, U.S.A. Nov. Pp. 350–346.Google Scholar
Peltola, H. (1978). Meningococcus group A vaccine in children three months to five years of age: Adverse reactions and immunogenicity related to endotoxin content and molecular weight of the polysaccharide. Journal of Pediatrics 92, 818–822.CrossRefGoogle Scholar
Pieroni, R. E. & Levine, L. (1976). Use of actinomycin B for quantitation of endotoxin in pertussis vaccine and extracts. International Symposium on Pertussis Series on Immunobiological Standardization 13, 198.Google Scholar
Rastogi, S. C., Hochstein, H. D. & Seligmann, E. B. Jr. (1977). Statistical determination of endotoxin content in influenza virus vaccine by the limulus amoebocyte lysate test. Journal of Clinical Microbiology 6, 144–148.CrossRefGoogle ScholarPubMed
Reichelderfer, P. S., Manischewitz, J. F., Wells, M. A., Hochstein, H. D. & Ennis, F. A. (1975). Reduction of endotoxin levels in influenza virus vaccines by barium sulfate adsorption-elution. Applied Microbiology 30, 333–334.CrossRefGoogle ScholarPubMed
Spasojevic, J. (1977). Study on toxicity of Bordetella pertussis cultures and antigens. Developmental Biological Standardization 34, 197–205.Google Scholar
WHO Secretariat (1953). Requirements for pertussis vaccine. World Health Organization Technical Report Series 61, 25–40.Google Scholar
Wong, K. H., Miller, C. E., Feeley, J. C. & Chan, Y. K. (1973). Determination of endotoxicity in bacterial vaccines. Applied Microbiology 25, 403–407.CrossRefGoogle ScholarPubMed