Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-14T04:25:56.343Z Has data issue: false hasContentIssue false
  • English
  • Français

Restriction endonuclease characterization of resistant plasmids in Enterobacteriaceae isolated from children in the Sudan

Published online by Cambridge University Press:  15 May 2009

P. Shears ,
G. Suliman  and
C. A. Hart
P. Shears
Affiliation:
University Department of Medical Microbiology, Royal Liverpool Hospital, Liverpool L7 8XW, U.K.
G. Suliman
Affiliation:
Children's Emergency Hospital, P.O. Box 412, Khartoum, Sudan
C. A. Hart
Affiliation:
University Department of Medical Microbiology, Royal Liverpool Hospital, Liverpool L7 8XW, U.K.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The investigation of plasmid similarity is an important component in the surveillance of antimicrobial resistance and in the detection of epidemic plasmids. The use of restriction endonucleases in the classification of transferable, multiply-resistant plasmids from faecal Enterobacteriaceae isolated at the Children's Emergency Hospital, Khartoum was investigated. Twenty-four transconjugant plasmids, coding for 11 different resistance patterns, each of molecular weight 62 MDa. were studied using four restriction enzymes; Pst I, Eco R I, Hind III and Ara II. Fifteen different digest profiles were obtained. Restriction profiles discriminated between plasmids with differing resistance patterns and demonstrated homology of plasmids with common resistance patterns. Restriction endonuclease digest patterns provide a potentially rapid and reproducible method of plasmid classification, that could contribute towards surveillance systems in tropical countries with a high prevalence of antimicrobial resistance.

Type
Research Article
Information
Epidemiology & Infection , Volume 103 , Issue 3 , December 1989 , pp. 487 - 496
Copyright
Copyright © Cambridge University Press 1989

References

REFERENCES

1.Farar, WE.Antibiotic resistance in developing countries. J Infect Dis 1985; 152: 1103–6.CrossRefGoogle Scholar
2.Levy, SB, Hedges, RW, Sullivan, F, Medeiros, AA, Sosroseputro, H.Multiple antibiotic resistance plasmids in Enterobacteriaceae isolated from diarrhoeal specimens of hospitalized children in Indonesia. J. Antimicrob Chemother. 1985; 16: 7–16.CrossRefGoogle ScholarPubMed
3.Shahid, NS, Rahaman, MM, Haider, K, Bann, H, Rahman, N.Changing pattern of resistant Shiga bacillus (Shigella dysenteriae Type 1) and Shigella flexneri in Bangladesh. J Infect Dis 1985; 152: 1114–19.CrossRefGoogle ScholarPubMed
4.Shears, P, Hart, CA, Broadhead, RL, Coulter, JBS.A note on antibiotic resistance in Escherichia coli isolated from children with diarrhoea in the Sudan. Ann Trop Paediat 1987; 7: 38–41.CrossRefGoogle ScholarPubMed
5.Shears, P, Hart, CA, Suliman, G.A preliminary investigation of antibiotic resistance in Enterobacteriaceae isolated from children with diarrhoea from four developing countries.Ann Trop Med Parasitol 1988; 82: 185–8.CrossRefGoogle ScholarPubMed
6.Frost, JA, Rowe, B, Vandepitte, J, Threlfall, EJ.Plasmid characterisation in the investigation of an epidemic caused by multiply resistant Shigella dysenteriae Type 1 in Central Africa. Lancet 1981; 2: 1074–6.CrossRefGoogle ScholarPubMed
7.Datta, N, Olarte, J.R factors in strains of Salmonella typhi and Shigella dysenteriae isolated in Mexico: classification by compatibility. Antimicrob Agents Chemother 1974; 5: 310–17.CrossRefGoogle ScholarPubMed
8.Crosa, JH, Olarte, J, Mata, LJ, Luttropp, LK, Penaranda, ME.Characterisation of an R-plasmid associated with ampicillin resistance in Shigella dysenteriae Type 1 isolated from epidemics. Antimicrob Agents Chemother 1977; 11: 553–8.CrossRefGoogle ScholarPubMed
9.World Health Organization. Control of antibiotic resistant bacteria. Bull WHO 1983; 61:423–33.Google Scholar
10.Datta, N. Plasmid classification: Incompatibility grouping. In: Timmis, KN, Puhler, A. eds. Plasmids of medical, environmental and commercial importance. Amsterdam: Elsevier. 1979; 3–12.Google Scholar
11.Frost, JA, Willshaw, GA, Barclay, EA, Rowe, B.Plasmid characterization of drug-resistant Shigella dysenteriae 1 from an epidemic in Central Africa. J Hyg 1985; 94: 163–72.CrossRefGoogle ScholarPubMed
12.Platt, DJ, Chesham, JS, Brown, DJ, Kraft, CA, Taggart, J.Restriction enzyme fingerprinting of enterobacterial plasmids: a simple strategy with wide application. J Hyg 1986; 97: 205–10.CrossRefGoogle ScholarPubMed
13.O'Brien, TF, Hopkins, JD, Gilleece, ES et al. Molecular epidemiology of antibiotic resistance in salmonella from animals and human beings in the United States. N EngI J Med 1982; 307: 1–6.CrossRefGoogle ScholarPubMed
14.Kado, CI, Liu, ST.Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 1981; 145: 1365–78.CrossRefGoogle ScholarPubMed
15.Shears, P, Suliman, G, Hart, CA.Occurrence of multiple antibiotic resistance and R plasmids in Enterobacteriaceae isolated from children in the Sudan. Epidemiol Infect 1988; 100: 73–81CrossRefGoogle Scholar
16.Birnboim, HC, Doly, J.A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 1979; 7: 1513–23.CrossRefGoogle Scholar
17.Hefron, F. Tn 3 and its relatives. In: Shapiro, JA, ed. Mobile genetic elements. New York: Academic Press, 1983; 223–60.Google Scholar
18.Richards, H, Nugent, M. The incidence and spread of Transposon Tn 7. In: Timmis, KN, Puhler, A, eds. Plasmids of medical, environmental and commercial importance. Amsterdam: Elsevier, 1979; 195–8.Google Scholar
19.Kraft, CA, Timbury, MC, Platt, DJ.Restriction enzyme fingerprinting of trimethoprim resistance plasmids. Epidemiol Infect 1987; 98: 241–52.CrossRefGoogle ScholarPubMed
20.Young, HK, Aymes, SGB.Plasmid trimethoprim resistance in Vibrio cholerae: migration of the type I dihydrofolate reductase gene out of the enterobacteriaceae. J Antimicrob Chemother 1986; 17: 697–703.CrossRefGoogle ScholarPubMed
21.Couturier, M, Bex, F, Bergquist, PL, Maas, WK.Identification and classification of bacterial plasmids. Microbiol Rev 1988; 52: 375–95.CrossRefGoogle ScholarPubMed
22.Broda, P, Plasmids. Oxford: W. H. Freeman 1979.Google Scholar
23.Timmis, KN, Gonzalez-Carrero, NI, Sekizaki, T, Rojo, F.Biological activities specified by antibiotic resistance plasmids. J Antimicrob Chemother 1986; 18: Suppl. C, 1–12.CrossRefGoogle ScholarPubMed
24.Taylor, DE, Chumpitaz, JC, Goldstein, F.Variability of IncHI1 plasmids from Salmonella typhi with special reference to Peruvian plasmids encoding resistance to trimethoprim and other antibiotics. Antimicrob Agents Chemother 1985; 28: 452–5.CrossRefGoogle ScholarPubMed
25.Chabert, YA, Roussel, A, Witchitz, JL, Le-Pors, MJ, Courvalin, P. Restriction endonuclease generated patterns of plasmids belonging to incompatibility groups I1,C,M and N; application to plasmid taxonomy and epidemiology. In: Timmis, KN, Puhler, A. eds. Plasmids of medical, environmental and commercial importance. Amsterdam: Elsevier. 1979.Google Scholar
26.Whiteley, M, Taylor, DE.Identification of DNA homologies among H incompatibility group plasmids by restriction enzyme digestion and Southern transfer hybridization. Antimicrob Agents Chemother 1983; 24: 194–200.CrossRefGoogle ScholarPubMed
27.Thompson, R, Hughes, SG, Broda, P.Plasmid identification using specific endonucleases. Mol Gen Genet 1974; 133: 141–9.CrossRefGoogle ScholarPubMed