Hostname: page-component-78c5997874-v9fdk Total loading time: 0 Render date: 2024-11-15T01:48:43.298Z Has data issue: false hasContentIssue false
  • English
  • Français

Serial subcultivation of Czechoslovakian and Japanese BCG strains

Published online by Cambridge University Press:  19 October 2009

T. W. Osborn
T. W. Osborn
Affiliation:
National Institute for Biological Standards and Control, Holly Hill, Hampstead, London NW3 6RB
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Changes in the Danish BCG strain under certain regimens of subculture have been shown in preceding studies to be associated with selection of a minority population. Three Czechoslovakian BCG strains, all originally derived from the Danish strain but thereafter and in distinction from it maintained on potato media, have now been investigated. Changes in the immunizing potency of two of these strains have been attributed by other workers to employment of the richer potato media in place of Sauton medium as used for maintenance of the parent Danish strain. However, results from the present study suggest rather that selection of a pre-existing minority genotype or of a now mutant occurred. This proposal is supported by the finding that the third strain has maintained characteristics similar to those of the Danish parent despite many previous transfers on potato media.

Another BCG strain investigated was the Japanese which, like the three Czechoslovakian strains, had been previously maintained on potato media. This strain has been shown in the present study to resemble the Danish strain in supporting a minority population yielding non-spreading colonies. Czechoslovakian vaccine prepared with seed culture supplied from Tokyo has retained characteristics similar to those of the Japanese parent. Although a majority population yielding spreading colonies appears so far to have been retained in both centres, it is considered that selection of the minority could still occur in the course of routine transfer.

Type
Research Article
Information
Journal of Hygiene , Volume 85 , Issue 2 , October 1980 , pp. 235 - 246
Copyright
Copyright © Cambridge University Press 1980

References

REFERENCES

Galuová, J. (1971). Biochemical properties of the Prnguo BCG strain. In International Symposium on BCG Vaccino, Frankfurt (Main) 1070: Symposia Scries in Itnmunobiological Standardisation 17, 117 (Karger, Baalo/Munich/Now York).Google Scholar
Ladefookd, A., Bunch-Chuistensen, K. & Guld, J. (1970). The protective effect in bank voles of flomo strains of BCG. Bulletin of the World Health Organization 43, 71.Google Scholar
Osborn, T. W. (1970). A study of somo effects of subculture on two BCG strains. Tubercle 57, 181.CrossRefGoogle Scholar
Osborn, T. W. (1979). Serial subculture of BCG on solid and liquid media. Tubercle 60, 83.Google Scholar
Pierce, Cynthia H. & Dubos, R. J. (1956). Differential characteristics in vitro and in vivo of sevoral substrains of BCG.ll. Morphologic characteristics in vitro and in vivo. American Eeview of Tuberculosis and Pulmonary Diseases 74, 667.Google Scholar
Průchová, J., Mára, M., Mohelská, H., Štr, Z. & Galliová, J. (1976). Somo biological, biochemical and morphological changes and their correlations in selected BCG vaccine strains. Journal of Biological Standardisation 4, 319.CrossRefGoogle Scholar
Ungar, J., Muggleton, P. W., Dudley, J. A. R. & Griffiths, Margaret I.. (1962). Preparation and properties of a freezo-dricd BCG vaccino of increased stability. British Medical Journal ii, 1086.CrossRefGoogle Scholar
World Health Organization (1972). Biological Substances–International Standards, Reference Preparations, and Reference Reagents, 1972, Geneva.Google Scholar