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Serological responses of chickens experimentally infected with Salmonella enteritidis PT4 by different routes

Published online by Cambridge University Press:  15 May 2009

H. Chart ,
A. Baskerville ,
T. J. Humphrey  and
B. Rowe
H. Chart
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
A. Baskerville
Affiliation:
Division of Pathology, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 OJG
T. J. Humphrey
Affiliation:
Public Health Laboratory, Church Lane, Exeter, Devon EX2 5AD
B. Rowe
Affiliation:
Division of Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK
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Summary

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Commercially reared chickens were challenged with Salmonella enteritidis phage-type (PT) 4 by aerosol, or via the conjunctiva. Inhalation of 2·9 × 102 or 4·2 × 103 S. enteritidis resulted in the production of IgG antibodies to the lipopolysaccharide (LPS) of S. enteritidis PT4. When the aerosol inoculum was increased to 2·4 × 105 bacteria per bird the antibody produced were predominantly of the IgM-class. Chickens challenged with 103S. enteritidis PT4 via the conjunctiva mounted only a poor immune response. Increasing the challenge dose to 108 S. enteritidis resulted in the production of high-titre serum antibodies of both the IgG and IgM classes. Results from this study suggest that aerosols containing small numbers of S. enteritidis PT4 might be responsible for intraflock infection of poultry.

Type
Research Article
Information
Epidemiology & Infection , Volume 109 , Issue 2 , October 1992 , pp. 297 - 302
Copyright
Copyright © Cambridge University Press 1992

References

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