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Studies on the inhibitory properties of sodium thioglycollate on the germination of wet spores of clostridia

Published online by Cambridge University Press:  15 May 2009

D. A. A. Mossel  and
H. Beerens
D. A. A. Mossel
Affiliation:
Central Institute for Nutrition and Food Research TNO, Zeist, The Netherlands
H. Beerens
Affiliation:
Laboratory of Anaerobic Bacteriology, Pasteur Institute, Lille, France
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Wet spores of fourteen strains of Clostridium, representing eight species, were enumerated in four different types of thioglycollate agar and in a similar medium in which thioglycollate was replaced by cysteine-HCl. Thioglycollate appeared to be toxic, in principle, to almost all strains tested, although the degree of toxicity was influenced by other components of the medium. These experiments entirely confirm earlier observations and must lead to the conclusion that the use of thioglycollate in sterility test media should be discontinued and that cysteine-HCl should be used as the redox potential reducing compound instead.

Type
Original Articles
Information
Journal of Hygiene , Volume 66 , Issue 2 , June 1968 , pp. 269 - 272
Copyright
Copyright © Cambridge University Press 1968

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