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Studies on the LS antigen of vaccinia virus

Published online by Cambridge University Press:  15 May 2009

C. J. M. Rondle  and
J. D. Williamson
C. J. M. Rondle
Affiliation:
Department of Bacteriology and Immunology, London School of Hygiene and Tropical Medicine
J. D. Williamson
Affiliation:
Virology Department, Wright–Fleming Institute of Microbiology, St Mary's Hospital Medical School, London
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A material (LS) analogous to that described by Craigie & Wishart (1936a) has been detected in extracts of vaccinia-infected rabbit dermis. Gel-diffusion tests on this material support the view that it is a single substance possessing two distinct serologically reactive sites. The tests also show that under some conditions rabbit LS may give two lines of precipitation against suitable antisera and that this is not due usually to the dissociation of LS into discrete L and S substances.

A study of vaccinia-infected sheep dermis failed to give evidence of a single LS substance. Instead two chemically different substances were isolated, one having the serological specificity of L, the other that of S. The physical, chemical and quantitative serological properties of sheep S were such that it could not have arisen by degradation of an LS material similar to that found in vaccinia-infected rabbit dermis. On this evidence it is suggested that vaccinia virus is not necessarily synthesized in the same way in different host tissues.

Type
Original Articles
Information
Journal of Hygiene , Volume 66 , Issue 3 , September 1968 , pp. 415 - 426
Copyright
Copyright © Cambridge University Press 1968

References

Baxby, D. & Rondle, C. J. M. (1968). The inhibition of growth of vaccinia and cowpox viruses in RK13 cells. J. Hyg., Camb. 66, 191–205.CrossRefGoogle Scholar
Ch'en, W. K. (1934). Preparation of the specific soluble substance from vaccinia virus. Proc. Soc. exp. Biol. Med. 32, 491–4.CrossRefGoogle Scholar
Cohen, G. H. & Wilcox, W. C. (1966). Soluble antigens of vaccinia-infected mammalian cells. J. Bact. 92, 676–86.CrossRefGoogle ScholarPubMed
Craigie, J. & Wishart, F. O. (1936 a). Studies on the soluble precipitable substances of vaccinia. I. The dissociation in vitro of soluble precipitable substances from the elementary bodies of vaccinia. J. exp. Med. 64, 803–18.CrossRefGoogle ScholarPubMed
Craigie, J. & Wishart, F. O. (1936 b). Studies on the soluble precipitable substances of vaccinia. II. The soluble precipitable substance of dermal filtrate. J. exp. Med. 64, 819–30.CrossRefGoogle Scholar
Craigie, J. & Wishart, F. O. (1938). The antigenic properties of vaccinia virus. J. Bact. 35, 25.Google Scholar
Gispen, R. (1955). Analysis of pox-virus antigens by means of double diffusion. A method for direct serological differentiation of cowpox. J. Immun. 74, 134–41.CrossRefGoogle ScholarPubMed
Harris, R., Harrison, G. A. & Rondle, C. J. M. (1963). Vaccinia virus and human blood-group-A substance. Acta. genet. Statist. med. 13, 44–57.Google ScholarPubMed
Jennings, R. K. (1954). Diffusion-precipitin studies of the complexity of antigen mixtures. I. The nature of bovine antigens in Dick toxin. J. Bact. 67, 565–70.CrossRefGoogle ScholarPubMed
Joklik, W. K. (1966). The poxviruses. Bact. Rev. 30, 33–66.CrossRefGoogle ScholarPubMed
Kaminski, M. (1955). Studies on egg white and its constituents by immunochemical techniques in gelified media: specific precipitation by double diffusion and immunoelectrophoretic analysis. J. Immun. 75, 367–76.CrossRefGoogle ScholarPubMed
McDuffie, F. C., Kabat, E. A., Allen, P. Z. & Williams, C. A. (1958). An immunochemical study of the relationship of human blood group isoantibodies to γ1- and γ2-globulins. J. Immun. 81, 48–64.CrossRefGoogle ScholarPubMed
Marquardt, J., Holm, S. E. & Lycke, E. (1965 a). Immunoprecipitating factors of vaccinia virus. Virology, 27, 170–8.CrossRefGoogle ScholarPubMed
Marquardt, J., Holm, S. E. & Lycke, E. (1965 b). Immunoprecipitating soluble vaccinia virus antigens. Arch. ges. Virusforsch. 15, 178–87.CrossRefGoogle ScholarPubMed
Nicoli, J. & Jolibois, C. (1964). Antigènes solubles des ‘pox virus’. I. Étude des antigènes solubles variolique et vaccinal. Annls Inst. Pasteur, Paris 107, 374–83.Google Scholar
Ornstein, L. & Davies, B. J. (1961). Preprint, Disc Electrophoresis, parts I and II. Distillation Products Industries, Eastman Kodak Company, Rochester, N.Y.Google Scholar
Parker, R. F. & Rivebs, T. M. (1937). Immunological and chemical investigations of vaccine virus. VI. Isolation of a heat-stable, serologically active substance from tissues infected with vaccine virus. J. exp. Med. 65, 243–9.CrossRefGoogle ScholarPubMed
Rondle, C. J. M. & Dumbell, K. (1962). Antigens of cowpox virus. J. Hyg., Camb. 60, 41–9.CrossRefGoogle ScholarPubMed
Shedlovsky, T. & Smadel, J. E. (1942). The LS-antigen of vaccinia. II. Isolation of a single substance containing both L- and S-activity. J. exp. Med. 75, 165–78.CrossRefGoogle Scholar
Smadel, J. E., Hoagland, C. J. & Shedlovsky, T. (1943). The LS-antigen of vaccinia. IV. Chemical analysis of LS and the effect of chymotrypsin on LS. J. exp. Med. 77, 165–71.CrossRefGoogle ScholarPubMed
Smadel, J. E. & Shedlovsky, T. (1942). Antigens of vaccinia. Ann. N.Y. Acad. Sci. 43, 35–46.CrossRefGoogle Scholar
Smith, W. (1932). A heat-stable precipitating substance extracted from vaccinia virus. Br. J. exp. Path. 13, 434–46.Google Scholar
Westwood, J. C. N., Zwartouw, H. T., Appleyard, G. & Titmus, D. H. J. (1965). Comparison of the soluble antigens and virus antigens of vaccinia virus. J. gen. Microbiol. 38, 45–53.CrossRefGoogle ScholarPubMed
Williamson, J. D. (1963). Vaccinia soluble antigen. Ph.D. thesis, Liverpool University.Google Scholar
Williamson, J. D. & Rondle, C. J. M. (1964). A virus-specific hapten from vaccinia-infected sheep dermis. Biochem. J. 90, 13–14.Google Scholar
Wilson, M. W. & Pringle, B. H. (1954). Experimental studies of the agar-plate precipitin test of Ouchterlony. J. Immun. 73, 232–43.CrossRefGoogle ScholarPubMed
Wilson, M. W. & Pringle, B. H. (1955). Interpretation of the Ouchterlony precipitin test. J. Immun. 75, 460–9.CrossRefGoogle ScholarPubMed