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The titration of vaccinia virus by the pock counting technique

Published online by Cambridge University Press:  15 May 2009

C. Kaplan  and
G. Belyavin
C. Kaplan
Affiliation:
The Lister Institute of Preventive Medicine, Elstree, Hertfordshire
G. Belyavin
Affiliation:
Department of Bacteriology, University College Hospital Medical School, London, W.C. 1
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Ninety-seven groups of five-membrane pock counts from 115 titrations of vaccinia virus preparations were examined in an attempt to identify the statistical distribution followed by such counts, and to obtain an estimate of the overall coefficient of variation of the technique.

It was found that the distribution of counts was neither Poissonian nor normal, and that the count variances ‘wandered’ so extensively that no valid estimate of the overall coefficient of variation was possible. Nor was it possible to assess the intrinsic reproducibility of the test, although it is empirically a useful one.

Type
Research Article
Information
Journal of Hygiene , Volume 55 , Issue 4 , December 1957 , pp. 494 - 501
Copyright
Copyright © Cambridge University Press 1957

References

REFERENCES

Beveridge, W. I. B. & Burnet, F. M. (1946). The cultivation of viruses and rickettsiae in the chick embryo. Spec. Rep. Ser. med. Res. Coun. Lond., no. 256.Google Scholar
Collier, L. H. (1955). The development of a stable smallpox vaccine. J. Hyg., Camb., 53, 76.Google Scholar
Davis, H. (1940). A quantitative bacteriological investigation of the Tyndallisation process. Quart. J. Pharm. 13, 14.Google Scholar
Fazekas de st Groth, S. & Moran, P. A. P. (1955). A mathematical model of virus-cell interaction. J. Hyg., Camb., 53, 291.Google Scholar
Fenner, F. & McIntyre, G. A. (1956). Infectivity titrations of myxoma virus in the rabbit and the developing chick embryo. J. Hyg., Camb., 54, 246.CrossRefGoogle ScholarPubMed
Kapsenberg, J. G. (1954). Het Kweken van Vaccinia-virus in geexplanteerd Weefsel. Amsterdam: (M.D. Thesis).Google Scholar
Kleczkowski, A. (1955). The statistical analysis of plant virus assays: a transformation to include lesion numbers with small means. J. gen. Microbiol. 13, 91.CrossRefGoogle Scholar
Miles, A. A. & Misra, S. S. (1938). The estimation of the bactericidal power of the blood. J. Hyg., Camb., 38, 732.Google ScholarPubMed
Moran, P. A. P. (1955). Measuring resistance to virus infection. J. Hyg., Camb., 53, 143.Google Scholar
Overman, J. R. & Tamm, I. (1956). Quantitative titration of vaccinia virus on the chorioallantoic membrane. J. Immunol. 76, 228.Google Scholar
Rubin, H. (1955). Quantitative relations between causative virus and cell in the Rous No. 1 chicken sarcoma. Virology, 1, 445.Google Scholar
Westwood, J. C. N., Phipps, P. H. & Boulter, E. A. (1957). The titration of vaccinia virus on the chorio-allantoic membrane of the developing chick embryo. J. Hyg., Camb., 55, 123.Google Scholar