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Use of an ammonia electrode to study bacterial deamination of amino acids with special reference to d-asparagine breakdown by campylobacters

Published online by Cambridge University Press:  19 October 2009

M. A. Karmali ,
A. Williams ,
P. C. Fleming ,
C. Krishnan  and
M. M. Wood
M. A. Karmali
Affiliation:
Departments of Bacteriology and Biostatistics and the Research Institute, the Hospital for Sick Children, Toronto, Canada, M5G 1X8, and the Department of Microbiology, the University of Toronto
A. Williams
Affiliation:
Departments of Bacteriology and Biostatistics and the Research Institute, the Hospital for Sick Children, Toronto, Canada, M5G 1X8, and the Department of Microbiology, the University of Toronto
P. C. Fleming
Affiliation:
Departments of Bacteriology and Biostatistics and the Research Institute, the Hospital for Sick Children, Toronto, Canada, M5G 1X8, and the Department of Microbiology, the University of Toronto
C. Krishnan
Affiliation:
Departments of Bacteriology and Biostatistics and the Research Institute, the Hospital for Sick Children, Toronto, Canada, M5G 1X8, and the Department of Microbiology, the University of Toronto
M. M. Wood
Affiliation:
Departments of Bacteriology and Biostatistics and the Research Institute, the Hospital for Sick Children, Toronto, Canada, M5G 1X8, and the Department of Microbiology, the University of Toronto
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Summary

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A method using an ammonia electrode is being developed for investigating the deamination of amino acids and amides by bacteria. Application of this method to Campylobacter jejuni and C. coli has led to the demonstration of d-asparaginase activity in some strains. This has allowed the subdivision of both species into d-asparaginase-positive and -negative biotypes. Even though the method is in the developmental stage, it was found to be generally reproducible and easy to perform. Areas for further improving the procedure have been identified. The ammonia electrode offers the theoretical possibility of investigating the breakdown of any amino acid by bacteria. It thus opens up a new and practical approach for separating species and strains, particularly in those bacterial groups that are difficult to subdivide by conventional means.

Type
Research Article
Information
Journal of Hygiene , Volume 93 , Issue 2 , October 1984 , pp. 189 - 196
Copyright
Copyright © Cambridge University Press 1984

References

Benjamin, J., Leaper, S., Owen, R. J. & Skirrow, M. B. (1983). Description of Campylobacter laridis, a new species comprising the nalidixic acid resistant thermophilic Campylobacter (NARTC) group. Current Microbiology 8, 231–238.CrossRefGoogle Scholar
Cowan, S. T. & Steel, K. J. (1974). Manual for the Identification of Medical Bacteria, 2nd ed.Cambridge University Press.Google Scholar
Gottschalk, G. (1979), Bacterial Metabolism. New York: Springer-Verlag.CrossRefGoogle Scholar
Harvey, S. M. (1980). Hippurate hydrolysis by Campylobacter fetus. Journal of Clinical Microbiology 11, 435–437.CrossRefGoogle ScholarPubMed
Hebert, G. A., Hollis, D. G., Weaver, R. E., Lambert, M. A., Blaser, M. J. & Moss, C. W. (1982). Thirty years of campylobacters: biochemical characteristics and a biotyping proposal for Campylobacter jejuni. Journal of Clinical Microbiology 15, 1005–1073.CrossRefGoogle Scholar
Karmali, M. A. & Skirrow, M. B. (1984). Taxonomy of the genus Campylobacter. In Campylobacter Infection in Man and Animals (ed. Butzler, J. P.), pp. 1–20. Boca Raton, Florida: CRC Press Inc.Google Scholar
Lior, H. (1983). A new extended biotyping scheme for Campylobacter jejuni, Campylobacter coli and ‘Campylobacter laridis’ (NARTC). In campylobacter, vol. II (ed. Pearson, A. D. et al. ), p. 42. London: PHLS.Google Scholar
Skirrow, M. B. & Benjamin, J. (1980). Differentiation of enteropathogenic campylobacter. Journal of Clinical Pathology 33, 1122.CrossRefGoogle ScholarPubMed
Smibert, R. M. (1974). Campylobacter. In Bergey's Manual of Determinative Bacteriology. 8th ed. (ed. Buchanan, R. E. and Gibbons, X. E.), pp. 207–212. Baltimore: Williams and Wilkins.Google Scholar
Veron, M. & Chatelain, R. (1973). Taxonomic study of the genus Campylobacter Sebald and Veron and designation of the neotype strain for the type species, Campylobacter fetus (Smith and Taylor) Sebald and Veron. International Journal of Systematic Bacteriology 23. 122–134.CrossRefGoogle Scholar