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The use of monoclonal antibodies in virology

Published online by Cambridge University Press:  25 March 2010

J. Oxford
J. Oxford
Affiliation:
National Institute for Biological Standards and Control, Holly Hill, Hampstead, London NW3 6RB
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Antibodies have been used for the last five decades in the laboratory diagnosis of a wide range of diseases caused by viruses and in detailed investigations of virus structure. However, immunological and serological assays have always had problems of interpretation, reproducibility and standardization, resulting partly from the unavoidable heterogeneity of the antibodies in the test. When a mouse, for example, is immunized with a virus, the animal may easily recognise 10–20 different antigenic determinants. As many as five distinct antibodies can be produced by the mouse against each determinant and these will often differ from antibodies made by another mouse against the same antigenic determinant. The method of lymphocyte fusion and the subsequent generation of monoclonal antibodies (Kohler & Milstein, 1975; Kohler, 1980) overcomes these limitations.

Type
Special Article
Information
Journal of Hygiene , Volume 88 , Issue 3 , June 1982 , pp. 361 - 368
Copyright
Copyright © Cambridge University Press 1982

References

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