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Use of pulsed-field gel electrophoresis for detecting differences in Staphylococcus aureus strain populations between dairy herds with different cattle importation practices

Published online by Cambridge University Press:  09 October 2002

J. R. MIDDLETON ,
L. K. FOX ,
J. M. GAY ,
J. W. TYLER  and
T. E. BESSER
J. R. MIDDLETON
Affiliation:
Department of Veterinary Medicine and Surgery, University of Missouri, Columbia, MO 65211, USA
L. K. FOX
Affiliation:
Department of Veterinary Clinical Sciences, Washington State University, Pullman, WA 99164, USA
J. M. GAY
Affiliation:
Department of Veterinary Clinical Sciences, Washington State University, Pullman, WA 99164, USA
J. W. TYLER
Affiliation:
Department of Veterinary Medicine and Surgery, University of Missouri, Columbia, MO 65211, USA
T. E. BESSER
Affiliation:
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
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Abstract

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The hypothesis tested was that dairy herds which import cattle for replacement or expansion have a higher prevalence of Staphylococcus aureus mastitis and a greater number of new Staphylococcus aureus strains enter their herds than closed herds. Fifteen commercial dairy herds were divided into four groups based on cattle importation practices. Composite foremilk samples were collected at 4-monthly intervals for 1 year from all lactating cattle. Additionally, foremilk samples were collected from cattle at parturition and skin swabs were taken from the udder of primiparous heifers. All samples were cultured for Staphylococcus aureus and isolates were strain-typed using pulsed-field gel electrophoresis. Herds that purchased replacement heifers had a higher prevalence of Staphylococcus aureus mastitis than herds that purchased lactating cattle for expansion (P = 0.02). Herds that purchased replacement heifers had more total strains of Staphylococcus aureus (P = 0.01) and more new strains (P = 0.04) enter the herd than closed herds.

Type
Research Article
Information
Epidemiology & Infection , Volume 129 , Issue 2 , October 2002 , pp. 387 - 395
Copyright
© 2002 Cambridge University Press