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Muscle injury and antioxidant status in sled dogs competing in a long-distance sled dog race

Published online by Cambridge University Press:  09 March 2007

KW Hinchcliff ,
PD Constable  and
RA DiSilvestro
KW Hinchcliff*
Affiliation:
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, 601 Tharp Street, Columbus, OH 43210, USA
PD Constable
Affiliation:
Department of Veterinary Clinical Veterinary Medicine, College of Veterinary Medicine, University of Illinois, Urbana, IL, USA
RA DiSilvestro
Affiliation:
Department of Human Nutrition, College of Human Ecology, The Ohio State University, Columbus, OH, USA
*
*hinchcliff.2@osu.edu
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Abstract

Exercise is associated with an increase in the production of oxidants that may be instrumental in the development of exertional rhabdomyolysis. We speculated that participation in a long-distance sled race would alter antioxidant capacity of dogs, in conjunction with increases in indices of rhabdomyolysis. The objective was to determine the effect of participation in a long-distance sled dog race on antioxidant capacity and plasma creatine kinase (CK) activity in sled dogs. This was a prospective, longitudinal study on a convenience sample of 57 Alaskan sled dogs participating in a 1600 km sled dog race. Blood samples were collected before racing (31 dogs) and after racing (39 dogs) for measurement of plasma vitamin E concentration; CK, aspartate aminotransferase (AST) and caeruloplasmin (CER) activity; and red-blood-cell (RBC) glutathione peroxidase (GPX) and superoxide dismutase (SOD) activity. CER and GPX activities after the race were 26% and 14% lower, respectively, than before racing whereas CK and AST were 300% and 170% greater. There was no change in plasma vitamin E concentration or RBC SOD activity. We conclude that completion of a long-distance sled dog race involving prolonged and repeated submaximal exercise results in a reduction in enzymatic antioxidant activity in the blood of sled dogs.

Keywords

creatinine kinasecaeruloplasminglutathione peroxidasesuperoxide dismutasevitamin E
Type
Research Article
Information
Equine and Comparative Exercise Physiology , Volume 1 , Issue 1 , February 2004 , pp. 81 - 85
Copyright
Copyright © Cambridge University Press 2004

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