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Protective role of zinc pretreatment in hepatotoxicity induced by halothane

Published online by Cambridge University Press:  01 October 2008

C. Unsal ,
J. B. Celik ,
H. Toy ,
H. Esen  and
S. Otelcioglu
C. Unsal
Affiliation:
Selcuk University, Meram Medical Faculty, Department of Anesthesiology and Reanimation, Konya, Turkey
J. B. Celik*
Affiliation:
Selcuk University, Meram Medical Faculty, Department of Anesthesiology and Reanimation, Konya, Turkey
H. Toy
Affiliation:
Selcuk University, Meram Medical Faculty, Department of Pathology, Konya, Turkey
H. Esen
Affiliation:
Selcuk University, Meram Medical Faculty, Department of Pathology, Konya, Turkey
S. Otelcioglu
Affiliation:
Selcuk University, Meram Medical Faculty, Department of Anesthesiology and Reanimation, Konya, Turkey
*
Correspondence to: Jale Bengi Celik, Department of Anesthesiology, Meram Medical Faculty, Selcuk University, 42080 Konya, Turkey. E-mail: celikcet@hotmail.com; Tel: +533 5656838; Fax: +90 0332 223 6181
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Summary

Background and objective

This study was designed to determine the protective effects of zinc on halothane-induced hepatotoxicity.

Methods

Forty-five male Sprague-Dawley rats were divided into three groups. The halothane group received normal drinking water and diet; the zinc-halothane group received 227 mg L−1 zinc sulphate in the drinking water and diet for 2 weeks; and the control group received normal diet and water. At the end of 2 weeks, rats were housed in an anaesthesia box and 1 MAC (minimum alveolar concentration) halothane was administered at 6 L min−1 in room air for 2 h. This was repeated 48 h later. After the rats were sacrificed, we measured alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyl transpeptidase, glutathione-S-transferase, serum electrolytes and bilirubin in samples. The degree of liver toxicity was assessed by light microscopic examination.

Results

We demonstrated a reduction of alanine aminotransferase, aspartate amino transferase, glutathione-S-transferase levels and a reduction in liver damage in the zinc-halothane group.

Conclusion

The study concludes that zinc has the potential to alleviate the toxic effects of halothane in rat liver.

Keywords

HALOTHANEZINCRATLIVERTOXICITY
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 25 , Issue 10 , October 2008 , pp. 810 - 815
Copyright
Copyright © European Society of Anaesthesiology 2008

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