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Differential effects of ageing on the EEG during pentobarbital and ketamine anaesthesia

Published online by Cambridge University Press:  01 October 2008

Y. Fu ,
L. Guo ,
J. Zhang ,
Y. Chen ,
X. Wang ,
T. Zeng ,
S. Tian  and
Y. Ma
Y. Fu
Affiliation:
Chinese Academy of Sciences, Kunming Institute of Zoology, Laboratory of Primate Neuroscience Research and Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming, Yunnan, PR China Graduate University of Chinese Academy of Sciences, Beijing, PR China
L. Guo
Affiliation:
Chinese Academy of Medical Sciences, Institute of Medical Biology, Department of Viral Immunology, Kunming, Yunnan, PR China
J. Zhang
Affiliation:
Chinese Academy of Sciences, Kunming Institute of Zoology, Laboratory of Primate Neuroscience Research and Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming, Yunnan, PR China
Y. Chen
Affiliation:
Chinese Academy of Sciences, Kunming Institute of Zoology, Laboratory of Primate Neuroscience Research and Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming, Yunnan, PR China
X. Wang
Affiliation:
Chinese Academy of Sciences, Kunming Institute of Zoology, Laboratory of Primate Neuroscience Research and Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming, Yunnan, PR China
T. Zeng
Affiliation:
Chinese Academy of Sciences, Kunming Institute of Zoology, Laboratory of Primate Neuroscience Research and Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming, Yunnan, PR China
S. Tian
Affiliation:
Chinese Academy of Sciences, Kunming Institute of Zoology, Laboratory of Primate Neuroscience Research and Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming, Yunnan, PR China
Y. Ma*
Affiliation:
Chinese Academy of Sciences, Kunming Institute of Zoology, Laboratory of Primate Neuroscience Research and Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming, Yunnan, PR China Chinese Academy of Sciences, Kunming Primate Research Center, Kunming, Yunnan, PR China Kunming Biomed International, Kunming, Yunnan, PR China
*
Correspondence to: Yuanye Ma, Laboratory of Primate Neuroscience Research, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, #32 Jiao Chang Dong Lu, Kunming, Yunnan, 650223, P.R. China. E-mail: yuanma0716@vip.sina.com; Tel: +86 871 5194464; Fax: +86 871 5191823
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Summary

Background and objectives

Pentobarbital and ketamine are commonly used in animal experiments, including studies on the effects of ageing on the central nervous system. The electroencephalogram is a sensitive measure of brain activity. The present study investigated, under anaesthesia induced by the two drugs, whether cortical electroencephalogram in aged rats differs from that in young rats.

Methods

Electroencephalogram was recorded for young (2–3 months) and aged (15–17 months) rats before and during pentobarbital (40 mg kg−1) or ketamine (100 mg kg−1) anaesthesia. The relative power in five frequency bands (delta: 2–4 Hz; theta: 4–8 Hz; alpha: 8–12 Hz; beta: 12–20 Hz; gamma: 20–100 Hz) was analysed, and then compared between the two age groups.

Results

In both age groups, pentobarbital anaesthesia induced an increase in relative power in alpha and beta bands and a decrease in the theta band, but the degree of these power variations was more marked in aged rats. Ketamine anaesthesia increased relative power in the delta band and decreased that in the theta band; these effects were significantly different between the two age groups, with aged rats showing more markedly decreased power in the theta band.

Conclusions

(a) Pentobarbital and ketamine modified cortical electrical activity in a different manner as a function of age; (b) the modification of electroencephalogram relative power with anaesthesia was identical in young and aged rats but quantitatively more marked in aged rats. These findings will be useful in designing experiments that assess pathological changes in the central nervous system during ageing.

Keywords

ELECTROENCEPHALOGRAMAGEDRATKETAMINEPENTOBARBITALANAESTHESIA GENERAL
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 25 , Issue 10 , October 2008 , pp. 826 - 833
Copyright
Copyright © European Society of Anaesthesiology 2008

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