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Effects of propofol and halothane on long-term potentiation in the rat hippocampus after transient cerebral ischaemia

Published online by Cambridge University Press:  01 December 2007

J. Kakehata ,
H. Togashi ,
T. Yamaguchi ,
Y. Morimoto  and
M. Yoshioka
J. Kakehata*
Affiliation:
Tonan Hospital, Department of Anesthesia, Sapporo, Japan Department of Anesthesiology and Critical Care Medicine, Sapporo, Japan
H. Togashi
Affiliation:
Hokkaido University Graduate School of Medicine, Department of Neuropharmacology, Sapporo, Japan Health Sciences University of Hokkaido, School of Pharmaceutical Sciences, Department of Pharmacology, Tobetsu-cho, Japan
T. Yamaguchi
Affiliation:
Hokkaido University Graduate School of Medicine, Department of Neuropharmacology, Sapporo, Japan
Y. Morimoto
Affiliation:
Department of Anesthesiology and Critical Care Medicine, Sapporo, Japan
M. Yoshioka
Affiliation:
Hokkaido University Graduate School of Medicine, Department of Neuropharmacology, Sapporo, Japan
*
Correspondence to: Jin Kakehata, Department of Anesthesia, Tonan Hospital, Kita-1 Nishi- 6, Cyuo-ku, Sapporo 060-0001, Japan. E-mail: jn-anesth@shonanboys.com; Tel: +81 11 231 2121; Fax: +81 11 231 2630
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Summary

Background

Propofol is reported to have protective effects on cerebral ischaemia-induced neuronal death. The aim of this study was to explore whether propofol and halothane can protect hippocampal neuronal function from ischaemic injury during general anaesthesia in rats.

Methods

Rats were divided into 2-vessel occlusion (incomplete cerebral ischaemia) and 4-vessel occlusion (complete cerebral ischaemia) groups consisting of three subgroups each (sham-operated, propofol and halothane groups). One hour after starting propofol 1 mg kg−1 min−1 with 30% O2 and N2 or halothane 0.8% in 30% O2 and N2 rats with or without bilateral vertebral artery occlusion had bilateral common carotid arteries occluded by vessel clips for 10 min. Anaesthesia was maintained for another 1 h. Seven days after ischaemia–reperfusion, hippocampal long-term potentiation in the perforant path–dentate gyrus synapse was determined as an index of cerebral outcome.

Results

In the propofol groups, the formation of long-term potentiation was significantly impaired in the 2-vessel and 4-vessel occlusion groups compared to the respective sham-operated groups (P < 0.01 and P < 0.05, respectively). Impaired formation of long-term potentiation in propofol groups was comparable to that in halothane groups. The formation of long-team potentiation in the propofol and halothane 2-vessel group was not significantly different from that in the awake 2-vessel group.

Conclusions

Propofol and halothane administered during ischaemia do not possess protective effects against hippocampal neuronal dysfunction induced by cerebral ischaemia–reperfusion as evaluated by our transient ischaemic rat models.

Keywords

ANESTHESIA INTRAVENOUSPROPOFOLHIPPOCAMPUSLONG-TERM POTENTIATIONINTRAOPERATIVE PERIOD
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 24 , Issue 12 , December 2007 , pp. 1021 - 1027
Copyright
Copyright © European Society of Anaesthesiology 2007

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References

1.Ramani, R, Todd, MM, Warner, DS. A dose–response study of the influence of propofol on cerebral blood flow, metabolism and the electroencephalogram in the rabbit. J Neurosurg Anesthesiol 1992; 4: 110–119.Google Scholar
2.Ravussin, P, Tempelhoff, R, Modica, PA, Bayer-Berger, MM. Propofol vs. tiopental–isoflurane for neurosurgical anesthesia: comparison of hemodynamics, CSF pressure, and recovery. J Neurosurg Anesthesiol 1991; 3: 85–95.Google Scholar
3.Murphy, PG, Myers, DS, Davies, MJ, Webster, NR, Jones, JG. The antioxidant potential of propofol (2,6-diisopropylphenol). Br J Anaesth 1992; 68: 613–618.Google Scholar
4.Hans, P, Deby, C, Deby-Dupont, G, Vrijens, B, Albert, A, Lamy, M. Effect of propofol on in vitro lipid peroxidation induced by different free radical generating systems: a comparison with vitamin E. J Neurosurg Anesthesiol 1996; 8: 154–158.Google Scholar
5.Peters, CE, Korcok, J, Gelb, AW, Wilson, JX. Anesthetic concentrations of propofol protect against oxidative stress in primary astrocyte cultures. Anesthesiology 2001; 94: 313–321.Google Scholar
6.Ito, H, Watanabe, Y, Isshiki, A, Uchino, H. Neuroprotective properties of propofol and midazolam, but not pentobarbital, onneuronal damage induced by forebrain ischemia, based on the GABA A receptors. Acta Anaesthesiol Scand 1999; 43: 153–162.Google Scholar
7.Velly, LJ, Guillet, BA, Masmejean, FM et al. . Neuroprotective effects of propofol in a model of ischemic cortical cell cultures. Anesthesiology 2003; 99: 368–375.Google Scholar
8.Pittman, JE, Sheng, H, Pearlstein, R, Brinkhous, A, Dexter, F, Warner, DS. Comparison of the effects of propofol and pentobarbital on neurologic outcame and cerebral infarct size after temporary focal ischemia in the rat. Anesthegiology 1997; 87: 1139–1144.Google Scholar
9.Yamaguchi, S, Midorikawa, Y, Okuda, Y, Kitajima, T. Propofol prevents delayed neuronal death following transient forebrain ischemia in gerbils. Can J Anaesth 1999; 46: 593–598.Google Scholar
10.Weir, DL, Goodchild, CS, Graham, DI. propofol: effects on indices of cerebral ischemia. J Neurosurg Anesthesiol 1989; 1: 284–289.Google Scholar
11.Saito, R, Graf, R, Hubel, K, Fujita, T, Rosner, G, Heiss, WD. Reduction of infarct volume by halothane: effect on cerebral blood flow or perifocal spreading depression-like depolarizations. J Cereb Blood Flow Metab 1997; 17: 857–864.Google Scholar
12.Hoffman, WE, Thomas, C, Albrecht, RF. The effect of halothane and isoflurane on neurologic outcome following incomplete cerebral ischemia in the rat. Anesth Analg 1993; 76: 279–283.Google Scholar
13.Warner, DS, Ludwig, PS, Pearlstein, R, Brinkhoust, A. Halothane reduces focal ischemic injury in the rat when brain temperature is controlled. Anesthesiology 1995; 82: 1237–1245.Google Scholar
14.Warner, DS, Zhou, JG, Ramani, R, Todd, MM. Reversible focal ischemia in the rat: effects of halothane, isoflurane, and methohexital anesthesia. J Cereb Blood Flow Metab 1991; 11: 794–802.Google Scholar
15.Bliss, TV, Lømo, T. Long-lasting potentiation of synaptic transmission in the dentate area of the anesthetized rabbit following stimulation of the perforant path. J Physiol 1973; 232: 331–356.Google Scholar
16.Togashi, H, Mori, K, Ito, Y et al. . Involvement of interleukin-1beta/nitric oxide pathway in the post-ischemic impairment of long-term potentiation of the rat hippocampus. Neurosci Lett 2001; 313: 133–136.Google Scholar
17.Mori, K, Suda, N, Togashi, H, Matsumotot, M, Saito, H, Yoshioka, M. Effects of bifemilane on incomplete cerbral ischemia-induced reduction of long-term potentiation in rat hippocampal neurones in vivo. Pharmacol Toxicol 1999; 85: 98–102.Google Scholar
18.Otani, H, Togashi, H, Jesmin, S et al. . Temporal effects of edaravone, a free radical scavenger, on transient ischemia-induced neuronal dysfunction in the rat hippocampus. Eur J Pharmacol 2005; 512: 129–137.Google Scholar
19.Pulsinelli, WA, Brierley, JB. A new model of bilateral hemispheric ischemia in the unanesthetized rat. Stroke 1979; 10: 267–271.Google Scholar
20.Zweifler, RM, Voorhees, ME, Mahmood, MA, Parnell, M. Rectal temperature reflects tympanic temperature during mild induced hypothermia in nonintubated subjects. J Neurosurg Anesthesiol 2004; 16: 232–235.Google Scholar
21.Mellergard, P, Nordstrom, CH. Intracerebral temperature in neurosurgical patients. Neurosurgery 1991; 28: 709–713.Google Scholar
22.Ohtaki, H, Nakamachi, T, Dohi, K et al. . Controlled normothermia during ischemia is important for the induction of neuronal cell death after global ischemia in mouse. Acta Neurochir Suppl 2006; 96: 249–253.Google Scholar
23.Paxinos, G, Watson, C. The Rat Brain in Stereotaxic Coordinates, 2nd edn. New York, USA: Academic Press, 1986: 85–88, 125 PubMed 127.Google Scholar
24.Yamazaki, T, Nakakimura, K, Matsumoto, K, Xiong, L, Ishikawa, T, Sakabe, T. Effects of graded supprssion of the EEG with propofol on the neurogical outcome following incomplete cerebral ischaemia in rats. Eur J Anaethessiol 1999; 16: 320–329.Google Scholar
25.De Paepe, P, Belpaire, FM, Rossel, MT, Van Hoey, G, Boon, PA, Buylaert, WA. Influence of hypovolemia on the pharmacokinetics and the electroencephalographic effect of propofol in the rat. Anesthesiology 2000; 93: 1482–1490.Google Scholar
26.Young, Y, Menon, DK, Tisavipat, N, Matta, BF, Jones, JG. Propofol neuroprotection in a rat model of ischemia reperfusion injury. Eur J Anaesthesiol 1997; 14: 320–326.Google Scholar
27.Mori, K, Yoshioka, M, Suda, N et al. . An incomplete cerebral ischemia produced a delayed dysfunction in the rat hippocamal system. Brain Res 1998; 795: 221–226.Google Scholar
28.Matsumoto, M, Tachibana, K, Togashi, H et al. . Serotonergic modulation of psychological stress-induced alteration in synaptic plasticity in the rat hippocampal CA1 field. Brain Res 2004; 1022: 221–225.CrossRefGoogle ScholarPubMed
29.Matsumoto, M, Togashi, H, Ohashi, S, Tachibana, K, Yamaguchi, T, Yoshioka, M. Chronic treatment with milnacipran reverses the impairment of synaptic plasticity induced by conditioned fear stress. Psychopharmacology 2005; 179: 606–612.Google Scholar
30.Bayona, NA, Gelb, AW, Jiang, Z, Wilson, JX, Urquhart, BL, Cechetto, DF. Propofol neuroprotection in cerebral ischemia and its effects on low-molecular-weight antioxidants and skilled motor tasks. Anesthesiology 2004; 100: 1151–1159.Google Scholar
31.Kochs, E, Hoffman, WE, Werner, C et al. . The effects of propofol on brain electrical activity, neurologic outcome, and neuronal damage following incomplete ischemia in rats. Anesthesiology 1992; 76: 245–252.Google Scholar
32.Ridenour, TR, Warner, DS, Todd, MM, Gionet, TX. Comparative effect of propofol and halotane on outcome from temporary middle cerebral artery occlusion in the rat. Anesthesiology 1992; 76: 807–812.Google Scholar
33.Gelb, AW, Bayona, NA, Wilson, JX, Cechetto, DF. Propofol anesthesia compared to awake reduces infarct size in rats. Anesthesiology 2002; 96: 1183–1190.Google Scholar