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Investigation of the relaxant effects of propofol on ovalbumin-induced asthma in guinea pigs

Published online by Cambridge University Press:  01 September 2007

I. Bagcivan ,
O. Cevit ,
M. K. Yildirim ,
S. Gursoy ,
S. Yildirim ,
T. Kaya  and
C. Mimaroglu
I. Bagcivan*
Affiliation:
Cumhuriyet University School of Medicine, Department of Pharmacology, Sivas, Turkey
O. Cevit
Affiliation:
Cumhuriyet University School of Medicine, Department of Pediatry, Sivas, Turkey
M. K. Yildirim
Affiliation:
Cumhuriyet University School of Medicine, Department of Pharmacology, Sivas, Turkey
S. Gursoy
Affiliation:
Cumhuriyet University School of Medicine, Department of Anesthesiology, Sivas, Turkey
S. Yildirim
Affiliation:
Cumhuriyet University School of Medicine, Department of Pharmacology, Sivas, Turkey
T. Kaya
Affiliation:
Cumhuriyet University School of Medicine, Department of Pharmacology, Sivas, Turkey
C. Mimaroglu
Affiliation:
Cumhuriyet University School of Medicine, Department of Anesthesiology, Sivas, Turkey
*
Correspondence to: Ihsan Bagcivan, Department of Pharmacology, Cumhuriyet University School of Medicine, 58140 Sivas, Turkey. E-mail: ibagcivan@cumhuriyet.edu.tr; Tel: +90 346 2191010; Fax: +90 346 2191155
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Summary

Background and objective

Because the incidence of asthma appears to be increasing, the importance of proper perioperative management of individuals with asthma will also continue to increase. Although its mechanism of smooth muscle relaxation is unknown, propofol has been associated with less bronchoconstriction during anaesthetic induction. The aim of this study was to investigate the possible mechanism of these effects and the effects of propofol on the isolated trachea preparations from control and ovalbumin-sensitized guinea pigs.

Methods

Adult male guinea pigs, weighing 280–330 g, were randomly allocated to two experimental groups, each consisting of 10 animals. Ten guinea pigs were sensitized by intramuscular injections of 0.30 mL of a 5% (w/v) ovalbumin/saline solution into each thigh (0.6 mL total) on days 1 and 4, whereas the remaining 10 served as controls receiving a total of 0.6 mL distilled water on days 1 and 4 as placebo. The isolated trachea preparations were mounted in tissue baths with modified Krebs–Henseleit solution and aerated with 95% oxygen and 5% carbon dioxide. We tested the effects of propofol (10−7–10−3 M) on resting tension and after precontraction with carbachol and histamine on isolated trachea preparations from control and ovalbumin-sensitized guinea pigs. We also tested the effect of propofol on isolated trachea preparations precontracted with carbachol and histamine in the absence and presence of different inhibitors or antagonists. We investigated propofol responses in tracheal smooth muscle precontracted with CaCl2.

Results

Propofol (10−7–10−3 M) produced a concentration-dependent relaxation of isolated tracheal preparations precontracted by carbachol (10−6 M) and histamine (10−6 M) in both groups. Preincubation with N(w)-nitro l-arginine methyl ester (3 × 10−5 M), indomethacin (10−5 M) or propranolol (10−4 M) did not produce a significant alteration on propofol-induced relaxation responses (P > 0.05), while preincubation with tetraethylammonium (3 × 10−4 M) significantly decreased the propofol-induced relaxation responses in both groups (P < 0.05). Propofol (10−7–10−3 M) induced concentration-dependently relaxations in isolated trachea rings precontracted with CaCl2 in both the control and ovalbumin-sensitized groups.

Conclusion

Propofol induced concentration-dependent relaxations in precontracted, isolated trachea smooth muscle of guinea pigs in both the control and ovalbumin-sensitized groups. These relaxations were independent of epithelial function and stimulation of β adrenergic receptors. Opened Ca2+-sensitive K+ channels and inhibited L-type Ca2+ channels can contribute to these relaxations.

Keywords

PROPOFOLTRACHEAGUINEA PIGSOVALBUMIN, sensitization
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 24 , Issue 9 , September 2007 , pp. 796 - 802
Copyright
Copyright © European Society of Anaesthesiology 2007

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References

1.Bousquet, J, Michel, FB. International consensus report on diagnosis and management of asthma. Allergy 1992; 47: 129–132.CrossRefGoogle ScholarPubMed
2.Yunginger, JW, Reed, CE, O’Connell, EJ et al. . A community-based study of the epidemiology of asthma. Am Rev Respir Dis 1992; 146: 888–894.CrossRefGoogle ScholarPubMed
3.Vener, DF, Long, T, Lerman, J. Perioperative respiratory complications after general anaesthesia in children with asthma. Can J Anaesth 1994; 41: A55.Google Scholar
4.Celiker, V, Basgul, E, Karakaya, G et al. . General anesthesia and postoperative pain management in analgesic intolerant patients with/without asthma: is it safe?. Allergol Immunopathol 2004; 32 (2): 64–68.CrossRefGoogle ScholarPubMed
5.Jyothi, T, Gramer, LC. Asthma, surgery, and general anesthesia: a review. J Asthma 2006; 43: 251–254.Google Scholar
6.Burr, ML, Butland, BK, King, S et al. . Changes in asthma prevalence: two surveys 15 years apart. Arch Dis Child 1989; 64: 1452–1456.CrossRefGoogle ScholarPubMed
7.Kuehni, CE, Davis, A, Brooke, AM et al. . Are all wheezing disorders in very young (preschool) children increasing in prevalence? Lancet 2001; 357: 1821–1825.CrossRefGoogle ScholarPubMed
8.Toelle, BG, Ng, K, Belousova, E et al. . Prevalence of asthma and allergy in schoolchildren in Belmont, Australia: three cross sectional surveys over 20 years. BMJ 2004; 328: 386–387.CrossRefGoogle ScholarPubMed
9.Brennan, LJ. Modern day-case anaesthesia for children. Br J Anaesth 1999; 83: 91–103.CrossRefGoogle ScholarPubMed
10.Warner, DO, Warner, MA, Barnes, RD et al. . Perioperative respiratory complications in patients with asthma. Anesthesiology 1996; 85: 460–467.CrossRefGoogle ScholarPubMed
11.Cigarini, I, Bonnet, F, Lorino, AM et al. . Comparison of the effects of fentanyl on respiratory mechanics under propofol or thiopental anaesthesia. Acta Anaesthesiol Scand 1990; 34: 253–256.CrossRefGoogle ScholarPubMed
12.Pizov, R, Brown, RH, Weiss, YS et al. . Wheezing during induction of general anesthesia in patients with and without asthma: a randomized, blinded trial. Anesthesiology 1995; 82: 1111–1116.CrossRefGoogle ScholarPubMed
13.Pederson, CM. The effect of sedation with propofol on postoperative bronchoconstriction in patients with hyperreactive airway disease. Intensive Care Med 1992; 18: 45–46.CrossRefGoogle Scholar
14.Conti, G, Utri, DD, Vilardi, V et al. . Propofol induces bronchodilation in mechanically ventilated chronic obstructive pulmonary disease (COPD) patients. Acta Anaesthesiol Scand 1993; 37: 105–109.CrossRefGoogle ScholarPubMed
15.Barnes, PJ. New concepts in the pathogenesis of bronchial hyperresponsiveness and asthma. J Allergy Clin Immunol 1989; 83 (6): 1013–1026.CrossRefGoogle ScholarPubMed
16.Gagnon, R, Lian, J, Boutin, Y, Hebert, J. Seasonal enhancement of IL-4 induced IgE synthesis by peripheral blood mononuclear cells of atopic patients. Clin Exp Allergy 1993; 23 (6): 498–503.CrossRefGoogle ScholarPubMed
17.Walker, C, Bauer, W, Braun, RK et al. . Activated T cells and cytokines in bronchoalveolar lavages from patients with various lung diseases associated with eosinophilia. Am J Respir Crit Care Med 1994; 150 (4): 1038–1048.CrossRefGoogle ScholarPubMed
18.Hessel, EM, Van Oosterhout, AJ, Hofstra, CL et al. . Bronchoconstriction and airway hyperresponsiveness after ovalbumin inhalation in sensitized mice. Eur J Pharmacol 1995; 293 (4): 401–412.CrossRefGoogle ScholarPubMed
19.Zhong-Xin, W, Daohong, Z, Gang, C et al. . Airway hyperresponsiveness to cigarette smoke in ovalbumin-sensitized guinea pigs. Am J Respir Crit Care Med 2000; 161: 73–80.Google Scholar
20.Hirshman, CA, Bergman, NA. Factors influencing intra pulmonary airway calibre during anaesthesia. Br J Anaesth 1990; 65: 30–42.CrossRefGoogle Scholar
21.Martin, TM, Nicolson, SC, Bargas, MS. Propofol anesthesia reduces emesis and airway obstruction in pediatric outpatients. Anesth Analg 1993; 76: 144–148.CrossRefGoogle ScholarPubMed
22.Hannallah, RS, Britton, JT, Schafer, PG et al. . Propofol anaesthesia in pediatric ambulatory patients: a comparison with thiopentone and halothane. Can J Anaesth 1994; 41: 12–18.CrossRefGoogle ScholarPubMed
23.Runcie, CJ, Mackenzie, SJ, Arthur, DS, Morton, NS. Comparison of recovery from anaesthesia induced in children with either propofol or thiopentone. Br J Anaesth 1993; 70: 192–195.CrossRefGoogle ScholarPubMed
24.Reimer, EJ, Montgomery, CJ, Bevan, JC et al. . Propofol anaesthesia reduces early postoperative emesis after pediatric strabismus surgery. Can J Anaesth 1993; 40: 927–933.CrossRefGoogle Scholar
25.Watcha, MF, Simeon, RM, White, PF, Stevens, JL. Effect of propofol on the incidence of postoperative vomiting after strabismus surgery in pediatric outpatients. Anesthesiology 1991; 75: 204–209.CrossRefGoogle ScholarPubMed
26.Bibault, P, Boisson-Bertrand, D, Duvivier, C et al. . Effect of the association propofol–alfentanil on bronchial resistances in asthmatic patients. Ann Fr Anesth Reanim 1991; 10: 264–268.CrossRefGoogle Scholar
27.Clarkson, K, Power, CK, O’Connell, F et al. . A comparative evaluation of propofol and midazolam as sedative agents in fiberoptic bronchoscopy. Chest 1993; 104: 1029–1031.CrossRefGoogle ScholarPubMed
28.Park, WK, Lynch, C, Johns, RA. Effects of propofol and thiopental in isolated rat aorta and pulmonary artery. Anesthesiology 1992; 77: 956–963.CrossRefGoogle ScholarPubMed
29.Gagar, N, Gok, S, Kalyongu, NI et al. . The effect of endothelium on the response to propofol on bovine coronary artery rings. Acta Anesthesiol Scand 1995; 39: 1080–1083.CrossRefGoogle Scholar
30.Chang, KSK, Davis, RF. Propofol produces endothelium-independent vasodilation and may act as a Ca2+channel blocker. Anesth Analg 1993; 76: 24–32.CrossRefGoogle ScholarPubMed
31.Oddera, S, Silvestri, M, Balbo, A et al. . Airway eosinophilic inflammation, epithelial damage, and bronchial hyperresponsiveness in patients with mild–moderate, stable asthma. Allergy 1996; 51: 100–107.Google ScholarPubMed
32.Davies, DE. The bronchial epithelium in chronic and severe asthma. Curr Allergy Asthma Rep 2001; 1: 127–133.CrossRefGoogle ScholarPubMed
33.Yamaguchi, M, Shibata, O, Nishioka, K et al. . Propofol attenuates Ovalbumin-induced smooth muscle contraction of the sensitized rat trachea: inhibition of serotonergic and cholinergic signaling. Anesth Analg 2006; 103: 594–600.CrossRefGoogle ScholarPubMed
34.Brayden, JE. Potassium channels in vascular smooth muscle. Clin Exp Pharmacol Physiol 1996; 23 (12): 1069–1076.CrossRefGoogle ScholarPubMed