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Levobupivacaine hydrochloride and sufentanil have no antimicrobial effect at 25°C in vitro

Published online by Cambridge University Press:  01 July 2007

M. Guillier ,
E. Boselli ,
L. Bouvet ,
J. Freney ,
F. N. R. Renaud ,
D. Chassard  and
B. Allaouchiche
M. Guillier
Affiliation:
Édouard Herriot Hospital, Department of Anaesthesiology and Intensive Care, Nosoco.tech, ISPB Université Lyon 1, EA 3090, Lyon, France
E. Boselli*
Affiliation:
Édouard Herriot Hospital, Department of Anaesthesiology and Intensive Care, Nosoco.tech, ISPB Université Lyon 1, EA 3090, Lyon, France University of Lyon, Nosoco.tech, ISPB Université Lyon 1, EA 3090, Lyon, France
L. Bouvet
Affiliation:
Édouard Herriot Hospital, Department of Anaesthesiology and Intensive Care, Nosoco.tech, ISPB Université Lyon 1, EA 3090, Lyon, France
J. Freney
Affiliation:
University of Lyon, Nosoco.tech, ISPB Université Lyon 1, EA 3090, Lyon, France
F. N. R. Renaud
Affiliation:
University of Lyon, Nosoco.tech, ISPB Université Lyon 1, EA 3090, Lyon, France
D. Chassard
Affiliation:
Édouard Herriot Hospital, Department of Anaesthesiology and Intensive Care, Nosoco.tech, ISPB Université Lyon 1, EA 3090, Lyon, France
B. Allaouchiche
Affiliation:
Édouard Herriot Hospital, Department of Anaesthesiology and Intensive Care, Nosoco.tech, ISPB Université Lyon 1, EA 3090, Lyon, France
*
Correspondence to: Emmanuel Boselli, Department of Anaesthesiology and Intensive Care, Édouard Herriot Hospital and University Claude Bernard Lyon I, Lyon 69003, France. E-mail: emmanuel.boselli@chu-lyon.fr; Tel: +33 472413172; Fax: +33 472413135
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Summary

Background and objectives

Levobupivacaine in combination with sufentanil may be used for labour or postoperative regional analgesia. The risk of bacterial growth within these contained solutions for several hours at room temperature is unknown. We investigated the in vitro antimicrobial effect of levobupivacaine and sufentanil against common micro-organisms encountered during regional anaesthesia.

Methods

Standardized suspensions of Staphylococcus aureus, Staphylococcus epidermidis and Escherichia coli were incubated for 1, 3, 6 and 24 h at 25°C, with saline (as control), sufentanil 0.5 or 0.75 μg mL−1, levobupivacaine hydrochloride 5.6 mg mL−1 and concentrations of 1.4, 2.8 and 5 mg mL−1 of levobupivacaine hydrochloride with sufentanil 0.5 μg mL−1. Colony counts were compared after 24 h incubation at 37°C.

Results

No bacterial growth was observed on any bacterial strain for any solution tested throughout the experiment.

Conclusions

Our results suggest that solutions of levobupivacaine combined with sufentanil may be used for 24 h at room temperature during regional anaesthesia with no risk of bacterial growth.

Keywords

ANTIMICROBIAL AGENTSLEVOBUPIVACAINESUFENTANILANAESTHESIA CONDUCTIONCOLONY COUNTMICROBIAL
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 24 , Issue 7 , July 2007 , pp. 634 - 639
Copyright
Copyright © European Society of Anaesthesiology 2007

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Footnotes

Presented in part at the American Society of Anesthesiologists Annual Meeting in Atlanta, GA, USA, October 22–26, 2005.

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