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Release of inflammatory mediators in irradiated cell salvage blood and their biological consequences in human beings following transfusion

Published online by Cambridge University Press:  23 December 2004

B. Beck-Schimmer ,
B. Romero ,
C. Booy ,
H. Joch ,
U. Haller ,
T. Pasch  and
D. R. Spahn
B. Beck-Schimmer
Affiliation:
University Hospital, Institute of Anaesthesiology, Zurich, Switzerland University Hospital, Institute of Physiology, Zurich, Switzerland
B. Romero
Affiliation:
University Hospital, Institute of Anaesthesiology, Zurich, Switzerland
C. Booy
Affiliation:
University Hospital, Institute of Physiology, Zurich, Switzerland
H. Joch
Affiliation:
University of Zurich, Institute of Physiology, Cardiovascular Research, Zurich, Switzerland
U. Haller
Affiliation:
University Hospital, Department of Gynaecology, Zurich, Switzerland
T. Pasch
Affiliation:
University Hospital, Institute of Anaesthesiology, Zurich, Switzerland
D. R. Spahn
Affiliation:
University Hospital, Institute of Anaesthesiology, Lausanne, Switzerland
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Summary

Background and objective: Irradiation of intraoperative cell salvage blood has recently been used to inactivate tumour cells before retransfusion, during cancer surgery. No information is available about a potential inflammatory response of the recipient to the retransfusion of irradiated intraoperative cell salvage blood. This pilot study was conducted to investigate the possible release of the pro-inflammatory mediators, tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β), eotaxin and monocyte chemo-attractant protein-1 (MCP-1), in the serum of recipients by intraoperative retransfusion of irradiated intraoperative cell salvage blood.

Methods: Nine patients undergoing gynaecological cancer surgery were included in this study. Intraoperative cell salvage blood was irradiated with 50 Gy and retransfused to the patient. Serum and intraoperative cell salvage blood concentrations of TNF-α, IL-1β, eotaxin and MCP-1 were repeatedly analysed before and after retransfusion, respectively before and after irradiation.

Results: Traces of mediators were detected in intraoperative cell salvage blood but no increase due to irradiation was observed. Following transfusion of intraoperative cell salvage blood, minute quantities (all <30 pg mL−1) of mediators were detected in the serum of patients. However, there was no significant upregulation compared to serum values before retransfusion.

Conclusions: These results provide evidence that retransfusion of irradiated intraoperative cell salvage blood might represent a blood-saving strategy in cancer surgery without an immunological inflammatory response as shown by a lack of upregulation of inflammatory mediators.

Keywords

BLOOD TRANSFUSION, autologousIMMUNOLOGICAL FACTORS, cytokinesINFLAMMATION MEDIATORS, chemokinesRADIATIONRADIOBIOLOGYSURGICAL PROCEDURES OPERATIVE, pelvic exenteration
Type
Original Article
Information
European Journal of Anaesthesiology , Volume 21 , Issue 1 , January 2004 , pp. 46 - 52
Copyright
2004 European Society of Anaesthesiology

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