Redfield revisited: variability of C[ratio ]N[ratio ]P in marine microalgae and its biochemical basis

Abstract

A compilation of data on the elemental composition of marine phytoplankton from published studies was used to determine the range of C[ratio ]N[ratio ]P. The N[ratio ]P ratio of algae and cyanobacteria is very plastic in nutrient-limited cells, ranging from <5 mol N[ratio ]mol P when phosphate is available greatly in excess of nitrate or ammonium to >100 mol N[ratio ]mol P when inorganic N is present greatly in excess of P. Under optimal nutrient-replete growth conditions, the cellular N[ratio ]P ratio is somewhat more constrained, ranging from 5 to 19 mol N[ratio ]mol P, with most observations below the Redfield ratio of 16. Limited data indicate that the critical N[ratio ]P that marks the transition between N- and P-limitation of phytoplankton growth lies in the range 20–50 mol N[ratio ]mol P, considerably in excess of the Redfield ratio. Biochemical composition can be used to constrain the critical N[ratio ]P. Although the biochemical data do not preclude the critical N[ratio ]P from being as high as 50, the typical biochemical composition of nutrient-replete algae and cyanobacteria suggests that the critical N[ratio ]P is more likely to lie in the range between 15 and 30. Despite the observation that the overall average N[ratio ]P composition of marine particulate matter closely approximates the Redfield ratio of 16, there are significant local variations with a range from 5 to 34. Consistent with the culture studies, lowest values of N[ratio ]P are associated with nitrate- and phosphate-replete conditions. The highest values of N[ratio ]P are observed in oligotrophic waters and are within the range of critical N[ratio ]P observed in cultures, but are not so high as to necessarily invoke P-limitation. The C[ratio ]N ratio is also plastic. The average C[ratio ]N ratios of nutrient-replete phytoplankton cultures, oceanic particulate matter and inorganic N and C draw-down are slightly greater than the Redfield ratio of 6·6. Neither the analysis of laboratory C[ratio ]N[ratio ]P data nor a more theoretical approach based on the relative abundance of the major biochemical molecules in the phytoplankton can support the contention that the Redfield N[ratio ]P reflects a physiological or biochemical constraint on the elemental composition of primary production.