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Evidence for an increase in functional platelet 5-HT2A receptors in depressed patients using the new ligand [125I]-DOI

Published online by Cambridge University Press:  16 April 2020

F. Serres ,
J.-M. Azorin ,
M. Valli  and
R. Jeanningros
F. Serres
Affiliation:
Inserm U 501, UER de Médecine Nord, Bd P. Dramard, 13916 Marseilles cedex 20, France
J.-M. Azorin
Affiliation:
Inserm U 501, UER de Médecine Nord, Bd P. Dramard, 13916 Marseilles cedex 20, France
M. Valli
Affiliation:
Inserm U 501, UER de Médecine Nord, Bd P. Dramard, 13916 Marseilles cedex 20, France
R. Jeanningros
Affiliation:
Inserm U 501, UER de Médecine Nord, Bd P. Dramard, 13916 Marseilles cedex 20, France
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Summary

Abnormalities in the serotonergic system have been implicated in the pathophysiology of depressive disorders. Human platelets possess serotonin-2A (5-HT2A) receptors, and previous research using LSD or ketanserin as ligands have indicated that their number is increased in depressed patients. Compared to other ligands previously used in platelet studies, DOI is highly selective for the 5-HT2A receptor and binds to its high-affinity state, therefore labeling only the receptors that are biologically coupled to the G-protein. We determined the density (Bmax) and the affinity (Kd) of 5-HT2A receptors labeled by [125I]-DOI in platelets from 21 untreated patients with major depression and 21 healthy volunteers. The density of the 5-HT2A binding sites was found to be increased in platelets from female depressed patients as compared to controls. No changes were observed in the Kd. We did not find any relationship between the binding parameters and either the severity of the depressive episode or the suicidal tendencies of the patients. Our results show that the number of coupled platelet 5-HT2A receptors is increased in depressed patients, indicating that platelet 5-HT2A receptor function is enhanced in depression.

Keywords

5-HT2A receptorsdepressionDOIplateletserotonin
Type
Original article
Information
European Psychiatry , Volume 14 , Issue 8 , December 1999 , pp. 451 - 457
Copyright
Copyright © Éditions scientifiques et médicales Elsevier SAS 1999

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