Hostname: page-component-78c5997874-mlc7c Total loading time: 0 Render date: 2024-11-10T08:47:00.213Z Has data issue: false hasContentIssue false
  • English
  • Français

GERMINATION OF UNTREATED AND PRIMED SEEDS IN RAPESEED (BRASSICA NAPUS VAR OLEIFERA DEL.) UNDER SALINITY AND LOW MATRIC POTENTIAL

Published online by Cambridge University Press:  06 January 2012

ROBERTA PACE ,
PAOLO BENINCASA ,
MICHEL EDMOND GHANEM ,
MURIEL QUINET  and
STANLEY LUTTS
ROBERTA PACE*
Affiliation:
Department of Agricultural and Environmental Sciences, University of Perugia, Borgo XX Giugno, 74 – 06121 Perugia, Italy
PAOLO BENINCASA
Affiliation:
Department of Agricultural and Environmental Sciences, University of Perugia, Borgo XX Giugno, 74 – 06121 Perugia, Italy
MICHEL EDMOND GHANEM
Affiliation:
Groupe de Recherche en Physiologie Végétale (GRPV), Earth and Life Institute – Agronomy (ELI-A), Université Catholique de Louvain, 5 (Bte 13) Place Croix du Sud, 1348-B Louvain-la-Neuve, Belgium
MURIEL QUINET
Affiliation:
Groupe de Recherche en Physiologie Végétale (GRPV), Earth and Life Institute – Agronomy (ELI-A), Université Catholique de Louvain, 5 (Bte 13) Place Croix du Sud, 1348-B Louvain-la-Neuve, Belgium
STANLEY LUTTS
Affiliation:
Groupe de Recherche en Physiologie Végétale (GRPV), Earth and Life Institute – Agronomy (ELI-A), Université Catholique de Louvain, 5 (Bte 13) Place Croix du Sud, 1348-B Louvain-la-Neuve, Belgium
*
‡Corresponding author. Email: pace.roberta@libero.it
Get access Rights & Permissions [Opens in a new window]

Summary

Salt (NaCl) and matric stress (polyethylene glycol; PEG 6000) impacts on germination were analysed in two rapeseed (Brassica napus var oleifera Del.) cultivars (Exagone: salt and water stress tolerant; Toccata: salt and water stress sensitive), which had the same imbibition kinetics when incubated in distilled water. Final germination percentage (G) and time to reach 50% G (T50) were affected either by 325 mM NaCl or −0.9 MPa PEG in Toccata but not in Exagone. Cultivars did not differ for protein concentration and protease activity in any germination condition; thus, protein metabolism seems not to be implicated in response to stress. Hydroxurea and cytochalasine-D inhibited germination in Toccata exposed to −0.9 MPa PEG but not in Exagone, suggesting that Exagone tolerance could be due to a lower amount of damaged DNA and lower requirement for DNA replication due to cell elongation rather than cell division for radicle elongation. Soluble sugars concentration increased in salt-stressed seeds from Exagone more than in Toccata and this could have allowed osmotic adjustment and therefore cell elongation despite the presence of external NaCl. Priming treatments with gibberellic acid, kinetin, NaCl and PEG hastened seed germination under control conditions but had no effect or even negative effects on germination under both salt and matric stresses.

Type
Research Article
Information
Experimental Agriculture , Volume 48 , Issue 2 , April 2012 , pp. 238 - 251
Copyright
Copyright © Cambridge University Press 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Afzal, I., Aslam, N., Hameed, F. M. A. and Ahmad, S. I. G. (2008). Enhancement of germination and emergence of canola seeds by different priming techniques. Caderno de Pesquisa Série Biologia 16:19–34.Google Scholar
Alam, M. Z., Stuchbury, T. and Naylor, R. E. L. (2002). Effect of NaCl and PEG-induced osmotic potentials on germination and early seedling growth of rice cultivars differing in salt tolerance. Pakistan Journal of Biological Sciences 5:1207–1210.CrossRefGoogle Scholar
Allen, R. G., Pereira, L. S., Raes, D. and Smith, M. (1998). Crop evapotranspiration – guidelines for computing crop water requirements. FAO Irrigation and Drainage Paper 56, Rome, Italy.Google Scholar
Almansouri, M., Kinet, J. M. and Lutts, S. (2001). Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Desf.). Plant and Soil 231:243–254.CrossRefGoogle Scholar
Al-Thabet, S. S., Leilah, A. A. and Al-Hawass, I. (2004). Effect of NaCl and incubation temperature on seed germination of three canola (Brassica napus L.) cultivars. Scientific Journal of King Faisal University 5:81–92.Google Scholar
Anastasi, U., Santonoceto, C. and Monti, M. (2003). Growth and yield water status and use of spring and winter rapeseed in relation to time of sowing. In Proceedings of the 11th International Rapeseed Congress, Copenhagen, Denmark, July 6–10, 3:805–807.Google Scholar
Andalibi, B., Zangani, E. and Nazari, A. H. (2005). Effects of water stress on germination indices in six rapeseed cultivars (Brassica napus L.). Iranian Journal of Agricultural Sciences 36:457–463.Google Scholar
Ashraf, M., Zafar, R. and Ashraf, M. Y. (2003). Time-course changes in the inorganic and organic components of germinating sunflower achenes under salt (NaCl) stress. Flora 198:26–36.CrossRefGoogle Scholar
Ashraf, M. Y., Afaf, R., Qureshi, M. S., Sarwar, G. and Naqvi, M. H. (2002). Salinity induced changes in α-amylase and protease activities and associated metabolism in cotton varietes during germination and early seedling growth stages. Acta Physiologiae Plantarum 24:37–44.CrossRefGoogle Scholar
Blackshaw, R. E. (1991). Soil, temperature and moisture effects on downey brome vs. winter canola, wheat and rye emergence. Crop Science 21:1034–1040.CrossRefGoogle Scholar
Bradford, M. (1976). A rapid and sensitive method for the quantification of microgram quatities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254.CrossRefGoogle Scholar
Bray, C. M., Davision, P. A., Ashraf, M. and Taylor, R. M. (1989). Biochemical changes during osmopriming of leak seeds. Annals of Botany 36:185–193.CrossRefGoogle Scholar
Burgass, R. W. and Powell, A. A. (1984). Evidence for repair processes in the invigoration of seed by hydration. Annals of Botany 53:753–757.CrossRefGoogle Scholar
Coolbear, P., Francis, A. and Grierson, D. (1984). The effect of low temperature pre-sowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds. Journal of Experimental Botany 35:1609–1617.CrossRefGoogle Scholar
Das, G. and Sen-Mandi, S. (1992). Scutellar amylase activity in naturally aged and accelerated aged wheat seeds. Annals of Botany 69:497–501.CrossRefGoogle Scholar
de Lespinay, A. (2009). Study of seed priming mechanisms of three plant species used in revegetation of industrial sites. PhD dissertation, Catholic University of Louvain, Louvain, Belgium.Google Scholar
de Lespinay, A., Lequeux, H., Lambillotte, B. and Lutts, S. (2010). Protein synthesis is differentially required for germination in Poa pratensis and Trifolium repens in the absence or in the presence of cadmium. Plant Growth Regulation 61:205–214.CrossRefGoogle Scholar
Dell'Aquila, A. and Bewley, J. D. (1989). Protein synthesis in the axes of polyethylene glycol-treated pea seed and during subsequent germination. Journal of Experimental Botany 40:1001–1007.CrossRefGoogle Scholar
Dell'Aquila, A. and Spada, P. (1993). The effect of salinity stress upon protein synthesis of germinating wheat embryos. Annals of Botany 72:97–101.CrossRefGoogle Scholar
FAO (2002). Agricultural Bulletin Board on Data Collection, Disemination and Quality of Statistics. Geneva: World Health OrganizationGoogle Scholar
Górnik, K., de Castro, R. D., Liu, Y., Bino, R. J. and Groot, S. P. C. (1997). Inhibition of cell division during cabbage (Brassica oleracea L.) seed germination. Seed Science Research 7:333–340.CrossRefGoogle Scholar
Gulzar, S. and Khan, A. (2001). Seed germination of halophytic grass Aeluropus lagopoides. Annals of Botany 87:319–324.CrossRefGoogle Scholar
Gupta, A. K., Singh, J., Kaur, N. and Singh, R. (1993). Effect of polyethylene glycol-induced water stress on germination and reserve carbohydrates metabolism in chickpea cultivars differing in tolerance to water deficit. Plant Physiology and Biochemistry 31:369–378.Google Scholar
Hampson, C. R. and Simpson, G. M. (1990). Effects of temperature, salt and osmotic potential on early growth of wheat (Triticum aestivum) I. Germination. Canadian Journal of Botany 68:524–528.CrossRefGoogle Scholar
Hardegree, S. P. and Emmerich, W. E. (1994). Seed germination response to polyethylene glycol solution depth. Seed Science and Technology 22:1–7.Google Scholar
Harris, D. (2006). Development and testing of “on-farm” seed priming. Advances in Agronomy 90:129–178.CrossRefGoogle Scholar
Huang, J. and Redmann, R. E. (1995). Salt tolerance of Hordeum and Brassica species during germination and early seedling growth. Canadian Journal of Plant Science 75:815–819.CrossRefGoogle Scholar
Jendrisak, J. (1980). The use of alpha-amanitin to inhibit in vivo RNA synthesis and germination in wheat embryos. Journal of Biological Chemistry 255:8529–8533.CrossRefGoogle ScholarPubMed
Jones, M. M. and Turner, N. C. (1980). Osmotic adjustment in expanding and fully expanded leaves of sunflower in response to water deficits. Australian Journal of Plant Physiology 7:181–192.Google Scholar
Kameli, A. and Lösel, D. M. (1996). Growth and sugar accumulation in durum wheat plants under water stress. New Phytologist 132:57–62.CrossRefGoogle ScholarPubMed
Katembe, W. J., Ungar, I. A. and Mitchell, J. P. (1998). Effect of salinity on germination and seedling growth of two Atriplex species (Chenopodiaceae). Annals of Botany 82:167–175.CrossRefGoogle Scholar
Kaya, M. D., Okc, G., Atak, M., Cıkılı, Y. and Kolsarıcı, O. (2006). Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy 24:291–295.CrossRefGoogle Scholar
Khan, M. A. and Rizvi, Y. (1994). Effect of salinity, temperature, and growth regulators on the germination and early seedling growth of Atriplex griffithii var. stocksii. Canadian Journal of Botany 72:475–479.CrossRefGoogle Scholar
Kumar, R. K., Sana, N. K. and Hossain, I. (2004). Ionic effect on mobilization of seed storage nutrient substances and lipase activity in germinating oil seeds (Brassica napus L.). Pakistan Journal of Biological Sciences 7:36–41.CrossRefGoogle Scholar
Lenormand, C., Martini, F. and Balangé, A. P. (1993). Germination of sunflower seeds: involvement of calcium. Plant Physiology and Biochemistry 31:447–455.Google Scholar
Li, Q., Feng, J. X., Han, P., Zhu, Y. X. (2006). Parental RNA is significantly degraded during Arabidopsis seed germination. Journal of Integrative Plant Biology 48:114–120.CrossRefGoogle Scholar
Lin, C. C. and Kao, C. H. (1995). NaCl stress in rice seedlings: starch mobilization and the influence of gibberellic acid on seedling growth. Botanical Bulletin of Academia Sinica 36:169–173.Google Scholar
Meyer, R. F. and Boyer, J. S. (1981). Osmoregulation, solute distribution and growth in soybean seedlings having low water potentials. Planta 151:482–489.CrossRefGoogle ScholarPubMed
Michel, B. E. and Kaufmann, M. R. (1973). The osmotic potential of polyethylene glycol 6000. Plant Physiology 51:914–916.CrossRefGoogle ScholarPubMed
Murillo-Amador, B., Lopez-Aguilar, R., Kaya, C., Larrinaga-Mayoral, J. and Flores-Hernandez, A. (2002). Comparative effects of NaCl and polyethylene on germination, emergence and seedling growth of cowpea. Journal of Agronomy and Crop Science 188:235–247.CrossRefGoogle Scholar
Nemati, I., Moradi, F., Gholizadeh, S., Esmaeili, M. A. and Bihamta, M. R. (2011). The effect of salinity stress on ions and soluble sugars distribution in leaves, leaf sheaths and roots of rice (Oryza sativa L.) seedlings. Plant, Soil and Environment 57:26–33.CrossRefGoogle Scholar
Onofri, A., Carbonell, E. A., Piepho, H.-P., Mortimer, A. M. and Cousens, R. D. (2010). Current statistical issues in weed research. Weed Research 50:5–24.CrossRefGoogle Scholar
Pace, R. and Benincasa, P. (2010). Effect of salinity and low osmotic potential on the germination and seedling growth of rapeseed cultivars with different stress tolerance. Italian Journal of Agronomy 5:69–77.CrossRefGoogle Scholar
Pradhan, S. P., Conrad, J. R., Paterek, J. R. and Srivastava, V. J. (1998). Potential of phytoremediation for treatment of PAHs in soil at MGP sites. Journal of Soil Contamination 7:467–480.CrossRefGoogle Scholar
Prisco, J. T. and Viera, G. H. E. (1976). Effect of NaCl salinity on nitrogenous compounds and protease during germination of Vigna sinensis seeds. Physiologia Plantarum 36:317–320.CrossRefGoogle Scholar
Rajjou, L., Gallardo, K., Debeaujon, I., Vandekerckhove, J., Job, C. and Job, D. (2004). The effect of alpha-amanitin on the Arabidopsis seed proteome highlights the distinct roles of stored and neosynthesized mRNAs during germination. Plant Physiology 134:1598–1613.CrossRefGoogle ScholarPubMed
Ruan, C. J., Teixeira da Silva, J. A., Mopper, S., Qin, P. and Lutts, S. (2010). Halophyte improvement for a salinized world. Critical Reviews in Plant Science 29:329–359.CrossRefGoogle Scholar
Schultz, C. and Small, J. G. C. (1991). Inhibition of lettuce seed germination by cycloheximide and chloramphenicol is alleviated by kinetin and oxygen. Plant Physiology 97:836–838.CrossRefGoogle ScholarPubMed
Thornton, J. M. and Powell, A. A. (1992). Short-term aerated hydration for the improvement of seed quality of Brassica oleracea. Seed Science Research 2:41–49.CrossRefGoogle Scholar
Tiryaki, I. (2006). Priming and storage of amaranth seeds: effects of plant growth regulators on germination performance at low temperature. Seed Science and Technology 34:169–179.CrossRefGoogle Scholar
Vicente, O., Boscaiu, M., Naranjo, M. A., Estrelles, E., Bellès, J. M. and Soriano, P. (2004). Responses to salt stress in the halophyte Plantago crassifolia (Plantaginaceae). Journal of Arid Environments 58:463–481.CrossRefGoogle Scholar
Willenborg, C. J., Gulden, R. H., Johnson, E. N. and Shirtliffe, E. N. (2004). Germination characteristics of polymer-coated canola (Brassica napus L.) seeds subjected to moisture stress at different temperatures. Agronomy Journal 96:786–791.CrossRefGoogle Scholar
Yemm, E. W. and Willis, A. J. (1954). The estimation of carbohydrates in plant extracts by anthrone. Biochemical Journal 57:508–514.CrossRefGoogle ScholarPubMed
Zheng, G. H., Gao, Y. P., Wilen, R. W. and Gusta, L. V. (1998). Canola seed germination and seedling emergence from pre-hydrated and re-dried seeds subjected to salt and water stresses at low temperature. Annals of Applied Biology 132:339–348.CrossRefGoogle Scholar