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Indigenous rhizobial strains SEMIA 4108 and SEMIA 4107 for common bean inoculation: A biotechnological tool for cleaner and more sustainable agriculture

Published online by Cambridge University Press:  16 April 2021

Bruno Britto Lisboa ,
Thomas Müller Schmidt ,
Arthur Henrique Ely Thomé ,
Raul Antonio Sperotto ,
Camila Gazolla Volpiano ,
Jackson Freitas Brilhante de São Jose ,
Luciano Kayser Vargas  and
Camille Eichelberger Granada Open the ORCID record for Camille Eichelberger Granada [Opens in a new window]
Bruno Britto Lisboa
Affiliation:
Department of Agricultural Research and Diagnosis, Agriculture, Livestock and Irrigation, Secretary of Rio Grande do Sul, Porto Alegre, RS, Brazil
Thomas Müller Schmidt
Affiliation:
University of Taquari Valley – Univates, Graduate Program in Biotechnology, Lajeado, RS, Brazil
Arthur Henrique Ely Thomé
Affiliation:
University of Taquari Valley – Univates, Graduate Program in Biotechnology, Lajeado, RS, Brazil
Raul Antonio Sperotto
Affiliation:
University of Taquari Valley – Univates, Graduate Program in Biotechnology, Lajeado, RS, Brazil
Camila Gazolla Volpiano
Affiliation:
Federal University of Rio Grande do Sul (UFRGS), Department of Genetics, Institute of Biosciences, Porto Alegre, RS, Brazil
Jackson Freitas Brilhante de São Jose
Affiliation:
Department of Agricultural Research and Diagnosis, Agriculture, Livestock and Irrigation, Secretary of Rio Grande do Sul, Porto Alegre, RS, Brazil
Luciano Kayser Vargas
Affiliation:
Department of Agricultural Research and Diagnosis, Agriculture, Livestock and Irrigation, Secretary of Rio Grande do Sul, Porto Alegre, RS, Brazil
Camille Eichelberger Granada*
Affiliation:
University of Taquari Valley – Univates, Graduate Program in Biotechnology, Lajeado, RS, Brazil
*
*Corresponding author. Email: cegranada@univates.br
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Summary

Inoculation of symbiotic N2-fixing rhizobacteria (rhizobia) in legumes is an alternative to reduce synthetic N fertiliser input to crops. Even though common bean benefits from the biological N2 fixation carried out by native rhizobia isolates, the low efficiency of this process highlights the importance of screening new strains for plant inoculation. Two rhizobial strains (SEMIA 4108 and SEMIA 4107) previously showed great potential to improve the growth of common beans under greenhouse conditions. Thus, this study evaluated the growth and grain yield of common bean plants inoculated with those strains in field experiments. The rhizobial identification was performed by 16S rRNA sequencing and the phylogeny showed that SEMIA 4108 and SEMIA 4107 are closely related to Rhizobium phaseoli, within a clade containing other 18 Rhizobium spp. type strains. Common bean plants inoculated with SEMIA 4107 showed similar productivity to N-fertilised (N+) plants in the first experiment (2016/17) and higher productivity in the second experiment (2018/19). The development of inoculated plants was different from that observed for N+. Nonetheless, comparing inoculated treatments with N-fertilised control, no yield or productivity losses at the end of the growing process were detected. Our results showed that inoculation of the rhizobial isolates SEMIA 4108 and SEMIA 4107 improved the growth and grain yield of common bean plants. The observed agronomical performance confirms that both strains were effective and can sustain common bean growth without nitrogen fertilisation under the edaphoclimatic conditions of this study.

Keywords

Biological nitrogen fixationIndigenous RhizobiumField experiments
Type
Research Article
Information
Experimental Agriculture , Volume 57 , Issue 1 , February 2021 , pp. 57 - 67
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Copyright
© The Author(s), 2021. Published by Cambridge University Press

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Footnotes

†

These authors contributed equally to this work.

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