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In vitro organogenesis from adult tissue of ‘Bahia’ sweet orange (Citrus sinensis L. Osbeck)

Published online by Cambridge University Press:  23 December 2006

Rosely Pereira da Silva ,
Weliton Antonio Bastos de Almeida ,
Elma dos Santos Souza  and
Francisco de Assis Alves Mourão Filho
Rosely Pereira da Silva
Affiliation:
Universidade de São Paulo (USP), Escola Superior de Agricultura Luiz de Queiroz (Esalq), Dep. de Produção Vegetal, Caixa Postal 9, CEP 13418-900 Piracicaba, SP, Brasil
Weliton Antonio Bastos de Almeida
Affiliation:
Universidade Federal da Bahia (UFBA), Faculdade de Agronomia, Dep. de Fitotecnia, CEP 44380-000 Cruz das Almas, BA, Brasil
Elma dos Santos Souza
Affiliation:
Universidade Federal da Bahia (UFBA), Faculdade de Agronomia, Dep. de Fitotecnia, CEP 44380-000 Cruz das Almas, BA, Brasil
Francisco de Assis Alves Mourão Filho
Affiliation:
Universidade de São Paulo (USP), Escola Superior de Agricultura Luiz de Queiroz (Esalq), Dep. de Produção Vegetal, Caixa Postal 9, CEP 13418-900 Piracicaba, SP, Brasil
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Abstract

Introduction. Plant regeneration from in vitro culture of adult citrus tissue may help in early evaluation of important horticultural characteristics of genetic material from breeding programs, which incorporate biotechnological tools. Our research aimed to optimize in vitro culture conditions that could lead to the maximum plant regeneration through organogenesis of ‘Bahia’ sweet orange (Citrus sinensis L. Osbeck) in explants (internodes and foliar disc segments) collected from adult plants grown under greenhouse conditions. Materials and methods. Organogenesis was induced in internodes on DBA3 culture media supplemented with BAP at (0.0, 1.0, 2.0 and 3.0) mg·L–1 combined with NAA (0.5 mg·L–1), and in foliar discs on culture media with BAP at (0.0, 0.5, 1.0, 1.5 and 2.0) mg·L–1 combined or not with NAA (0.5 mg.L–1). Results and discussion. The concentrations of (1.0, 2.0 or 3.0) mg·L–1 of BAP combined with 0.5 mg·L–1 of NAA induced in vitro organogenesis in internodes of ‘Bahia’ sweet orange. The use of foliar discs was not efficient for adventitious organogenesis.

Keywords

BrazilCitrus sinensismicropropagationin vitro regenerationexplantsculture mediaorganogenesisBrésilCitrus sinensismicropropagationrégénération in vitroexplantmilieu de cultureorganogenèse
Type
Research Article
Information
Fruits , Volume 61 , Issue 6 , November 2006 , pp. 367 - 371
Copyright
© CIRAD, EDP Sciences, 2006

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