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Physiological changes in relation to growth and ripening of khirni [Manilkara hexandra (Roxb.) Dubard] fruit

Published online by Cambridge University Press:  09 June 2009

Prakash Ramanbhai Patel  and
Tadapaneni Venkata Ramana Rao
Prakash Ramanbhai Patel
Affiliation:
Dep. Biosciences, Sardar Patel Univ., Vallabh Vidyanagar, Gujarat – 388120, India
Tadapaneni Venkata Ramana Rao
Affiliation:
Dep. Biosciences, Sardar Patel Univ., Vallabh Vidyanagar, Gujarat – 388120, India
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Abstract

Introduction. Fruit ripening is the process resulting in changes in color, taste and texture, which make the fruit acceptable for consumption. Since a wide spectrum of physiological, biochemical and organoleptic changes are involved in the development of a soft, edible, ripe fruit, we studied theses changes in an underutilized fruit, khirni [Manilkara hexandra (Roxb.) Dubard]. Materials and methods. The changes in biochemical composition, which includes chlorophylls, carotenoids, anthocyanins, sugars, starch, free amino acids, phenols and proteins, and the specific activity of enzymes such as amylase, invertase, catalase, peroxidase, pectinmethylesterase, polygalacturanase and cellulase were analyzed in the fruit of Manilkara hexandra at five sequential developmental stages (young, premature, mature, preripened and ripened fruit stages). Results and discussion. The pulp of khirni fruit tastes sour during its growth period, but turns sweet when it ripens. A decreasing trend in chlorophylls occurs simultaneously with an increase in the quantity of total carotenoids and anthocyanins. Further, an increase in the quantity of sugars, proteins and phenols occurs towards the ripened stage, but starch and total free amino acids show a decrease in their quantities. Also, khirni fruit exhibits climacteric behavior with its increased rate of respiration and ethylene production. The moderate to significant changes in the activity of enzymes such as amylase, invertase, catalase and peroxidase involved in a number of catabolic and anabolic reactions indicate that these enzymes also have an active role in the process of khirni fruit growth and ripening.

Keywords

IndiaManilkara hexandrafruitmaturationplant physiologygrowthIndeManilkara hexandrafruitmaturationphysiologie végétalecroissance
Type
Research Article
Information
Fruits , Volume 64 , Issue 3 , May 2009 , pp. 139 - 146
Copyright
© CIRAD, EDP Sciences, 2009

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References

Seymour, G.B., Manning, K., Eriksson, E.M., Popovich, A.H., King, G.J., Genetic identification and genomic organization of factors affecting fruit texture, J. Exp. Bot. 53 (2002) 2065–2071. CrossRef
Malik K.A., Sapotaceae, in: Nasir E., Ali S.I. (Eds.), Flora of Pakistan, No. 163. National Herbarium, PARC, Islamabad Dep. Bot., Univ. Karachi, Pakistan, 1984, p. 12.
Arora R.K., Pandey A., Wild edible plants of India: diversity, conservation and use, Natl. Bur. Plant Genet. Res., New Delhi, India, 1996, 132 p.
Singh, A.K., Shukla, S.K., Bajpai, A., Singh, A., Singh, M.P., Cultivating khirnee – A crop for diversification, Ind. Hortic. 52 (5) (2007) 4–5.
Kirtikar K.R., Basu B.D., Indian medicinal plants, Vol. 2, Int. Books Distrib., Dehradun, India, 2006, pp. 1496–1497.
Rastogi R.P., Mehrotra B.N., Compendium of Indian medicinal plants, Vol. 2, Cent. Drug Res. Inst., Lucknow, India, 1993, p. 444.
Vijayan R., Bedi S.J., Effect of chlorine pollution on three fruit tree species at Ranoli near Baroda, “India”. Environ. Pollut. 57 (2) (1989) 97–102.
Thimmaiah S.K., Standard methods of biochemical analysis, Kalyani Publ., New Delhi, India, 1999, pp. 49–310.
Wang, Zhong-Feng, Ying, Tie-Jin, Bao, Bi-Li, Huang, Xiao-Dan, Characteristics of fruit ripening in tomato mutant epi, J. Zhejiang Univ. Sci. 68 (6) (2005) 502–507. CrossRef
Moore, S., Stein, W.H., Photometric ninhydrin method for use in the chromatography of amino acids, J. Biol. Chem. 176 (1948) 367–388.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the folin phenol reagent, J. Biol. Chem. 193 (1951) 265.
Bray, H.C., Thorpe, W., Analysis of phenolic compounds of interest in metabolism, Meth. Biochem. Anal. 1 (1954) 27–52.
Teitel, D.C., Aharoni, Y., Barkai-Golan, R., The use of heat treatments to extend the shelf life of ‘Galia’ melons, J. Hortic. Sci. 64 (1989) 367–372. CrossRef
Devi P., Principles and methods in plant molecular biology, biochemistry and genetics, Agrobios, Jodhpur, India, 2001, pp. 31–72.
Bliss C.I., Statistics in biology, statistical methods for research in the natural sciences, Vol. 1, McGraw Hill Book Co., NY, USA, 1967, 558 p.
Willis R.B.H., Mc Glasson W.B., Graham D., Lee T.H., Hall E.G., Postharvest – an introduction to the physiology and handling of fruit and vegetables, CBS Publ. Distrib., New Delhi, India, 1996, pp. 17–38.
Stanley J.K., Postharvest physiology of perishable plant products, CBS Publ. Distrib., New Delhi, India, 1998, pp. 143–256.
Singh R.R., Sharma R.M., Structure, cellular components and composition of fruits and vegetables, in: Verma L.R., Joshi V.K. (Eds.), Postharvest technology of fruits and vegetables, Indus Publ. Co., New Delhi, India, 2000, pp. 76–93.
Mazumdar B.C., Majumder K., Methods on physico-chemical analysis of fruits, Daya Publ. House, Delhi, India, 2003, pp. 93–139.
Mattoo A.K., Murata T., Pantastico E.B., Chachin K., Ogata K., Phan C.T., Chemical changes during ripening and senescence, in: Pantastico E.B. (Ed.), Postharvest physiology, handling and utilization of tropical and subtropical fruits and vegetables, AVI Publ. Co. Inc., Westport, Conn., USA, 1975, pp. 103–127.
Hulme A.C., The Biochemistry of fruits and their products, Vol. 1, Acad. Press, London, UK, 1970, pp. 1–31.
Hansen E., Proteins, in: Hulme A.C. (Ed.), The biochemistry of fruits and their products, Vol. 1, Acad. Press, London, UK, 1970, pp. 147–158.
Frankel, C., Klein, I., Dilley, D.R., Protein synthesis enzymes in pome fruits, Plant Physiol. 43 (1968) 11–46.
Dilley D.R., Hypobaric storage – A new concept for preservation of perishables, Mich. State Hortic. Annu. Rep., 1972, 82 p.
Kidd F., West C.A., The courses of respiratory activity throughout the life of apple, Annu. Rep. Food Invest. Board, London, UK, 1925, pp. 27–33.
Bowler, C., Van-Montagu, M., Inze, D., Superoxide dismutase and stress tolerance, Annu. Rev. Plant Physiol. Plant Mol. Biol. 43 (1992) 83–116. CrossRef
Jimenez, A., Cressen, G., Kular, B., Firmin, J., Robinson, S., Verhoeyen, M., Mullineaux, P., Changes in oxidative process and components of the antioxidant system during tomato fruit ripening, Planta. 214 (2002) 751–758. CrossRef
Award, M., Young, R.E., Postharvest variation in cellulose, polygalacturonase and pectinmethylesterase in avocado fruits in relation to respiration and ethylene production, Plant Physiol. 64 (1979) 306–308.