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Allozymic variability in Spanish populations of Ceratitis capitata

Published online by Cambridge University Press:  15 July 2004

Carmen Callejas  and
M. Dolores Ochando
Carmen Callejas
Affiliation:
Dpto. de Genética, Fac. CC. Biológicas, Universidad Complutense, 28040 Madrid, Spain
M. Dolores Ochando
Affiliation:
Dpto. de Genética, Fac. CC. Biológicas, Universidad Complutense, 28040 Madrid, Spain
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Abstract

Introduction. The Tephritidae Ceratitis capitata is one of the most important agricultural pests in the world. Eradication programmes need as much genetic information as possible to be efficient. It is vital to know the genetic variation that exists in the areas thought to have been important in the expansion of the species, such as the Iberian Peninsula. The aim of this study was to determine the genetic structure of Spanish C. capitata populations and the relationships between them. Materials and methods. We studied the genetic variability of populations of C. capitata (eight wild samples and one laboratory strain) through the use of the horizontal starch gel electrophoresis technique and the assay of fifteen enzyme-coding loci chosen at random. Results. The quantity of variability detected in this polyphagous species was not high. Of the 15 loci studied, only four were clearly polymorphic. No significant differences were found in any comparison of populations collected from different hosts. Discussion. The distribution patterns of this variation seem to be the result of gene flow and selection in the form of agricultural practices.

Keywords

SpainCeratitis capitataelectrophoresisgenetic variationEspagneCeratitis capitataélectrophorèsevariation génétique
Type
Research Article
Information
Fruits , Volume 59 , Issue 3 , May 2004 , pp. 181 - 190
Copyright
© CIRAD, EDP Sciences

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References

Fimiani P., Mediterranean region, in: Robinson A.S., Hooper G. (Eds.), Fruit flies: their biology, natural enemies and control, vol. A (World crop pests; 3A), Elsevier Sci. Pub., Amsterdam, Netherlands, 1989.
Hagen, K.S., William, W.W., Tassan, R.L., Mediterranean fruit fly: the worst may be yet to come, Calif. Agric. 35 (1981) 5–7.
Fletcher B.S., Life history strategies of thephritid fruit flies, in: Robinson A.S., Hooper G. (Eds.), Fruit flies: their biology, natural enemies and control, vol. A (World crop pests; 3A), Elsevier Sci. Pub., Amsterdam, Netherlands, 1989.
Meixner, M.D., McPheron, B.A., Silva, J.G., Gasparich, G.E., Sheppard, W.S., The Mediterranean fruit fly in California: evidence for multiple introductions and persistent populations based on microsatellite and mitochondrial DNA variability, Mol. Ecol. 11 (2002) 891–899. CrossRef
Anonymous, Fruit fly pests: a world assessment of their biology and management, McPheron B.A., Steck G.J. (Eds.), St. Lucie Press, Delray Beach, USA, 1996.
Menken, S.B.J., Ulenberg, S.A., Biochemical characters in agricultural entomology, Agric. Zool. Rev. 2 (1987) 305–360.
Anonymous, Electrophoretic studies on agricultural pests, Loxdale H.D., Hollander J.D. (Eds.), Syst. Ass., Spec. Vol., Clarendon Press, Oxford, UK, 1989.
Milani R., Gasperi G., Malacrida A.R., Biochemical genetics (of Ceratitis capitata), in: Robinson A.S., Hooper G. (Eds.), Fruit flies: their biology, natural enemies and control, vol. A (World crop pests; 3A), Elsevier Sci. Pub., Amsterdam, Netherlands, 1989.
Haymer, D.S., He, M., McInnis, D.O., Genetic marker analysis of spatial and temporal relationships among existing populations and new infestations of the Mediterranean fruit fly (Ceratitis capitata), Heredity 79 (1997) 302–309. CrossRef
Malacrida, A.R., Guglielmino, C.R., Gasperi, G., Baruffi, L., Milani, R., Spatial and temporal differentiation in colonizing populations of Ceratitis capitata, Heredity 69 (1992) 101–111. CrossRef
Malacrida, A.R., Marinoni, F., Torti, C., Gomulski, L.M., Sebastian, F., Bonvicini, C., Gasperi, G., Guglielmino, C.R., Genetic aspects of the worldwide colonization process of Ceratitis capitata, J. Hered. 89 (1998) 501–507. CrossRef
Sheppard, W.S., Steck, G.J., McPheron, B.A., Geographic populations of the medfly may be differentiated by mitochondrial DNA variation, Experientia 48 (1992) 1010–1013. CrossRef
Gasparich, G.E., Silva, J.G., Han, H.-Y., McPheron, B.A., Steck, G.J., Sheppard, W.S., Population genetic structure of Mediterranean fruit fly (Diptera: Tephritidae) and implications for worldwide colonization patterns, Ann. Entomol. Soc. Am. 90 (1997) 790–797. CrossRef
Roda, P., Callejas, C., Reyes, A., Ochando, M.D., Caracterización genética en Ceratitis capitata asociada a fruto hospedador. I. Análisis isoenzimatico, Bol. Sanit. Veg. Plagas 22 (1996) 28–36.
Reyes, A., Ochando, M.D., Use of molecular markers for detecting the geographical origin of Ceratitis capitata (Diptera: Tephritidae) populations, Ann. Entomol. Soc. Am. 91 (1998) 222–227. CrossRef
Davies, N., Villablanca, F.X., Roderick, G.K., Bioinvasions of the Medfly Ceratitis capitata: source estimation using DNA sequences at multiple intron loci, Genetics 153 (1999) 351–360.
He, M., Haymer, D.S., Genetic relationships of populations and the origins of new infestations of the Mediterranean fruit fly, Mol. Ecol. 8 (1999) 1247–1257. CrossRef
Bonizzoni, M., Zheng, L., Guglielmino, C.R., Haymer, D.S., Gasperi, G., Gomulski, L.M., Malacrida, A.R., Microsatellite analysis of medfly bioinfestations in California, Mol. Ecol. 10 (2001) 2515–2524. CrossRef
Reyes, A., Ochando, M.D., A study of gene-enzyme variability in three Spanish populations of Ceratitis capitata, Int. Organ. Biol. Integr. Control Noxious Anim. Plants / West Paleartic Reg. Sect. (IOBC/wprs) Bull. 17 (1994) 151–160.
Ochando M.D., Reyes A., Callejas C., Genetic structure of Ceratitis capitata species: within and between populations variability, IOBC/wprs Bull. (in press).
Ayala, F.J., Powell, J.R., Tracey, M.L., Mourao, C.A., Perez-Salas, S., Enzyme variability in the Drosophila willistoni group. IV. Genetic variation in natural populations of Drosophila willistoni, Genetics 70 (1972) 113–139.
Poulik, M. D., Stach gel electrophoresis in a discontinuous system of buffers, Nature 180 (1957) 1477–1479. CrossRef
Nei, M., Genetic distance between populations, Am. Nat. 106 (1972) 283–292. CrossRef
Wright S., Evolution and the genetics of populations, vol. 4, Variability within and among natural populations, The Univ. of Chicago Press, Chicago, USA, 1978.
Singh R.S., Rhomberg L.R., A comprehensive study of genetic variation in natural populations of Drosophila melanogaster. II. Estimates of heterozygosity and patterns of geographic differentiation, Genetics, 117 (1987) 255–271.
Powell, J.P., Genetic polymorphisms in varied environments, Science 174 (1971) 1035–1036. CrossRef
Gillespie, J.H., Langley, C.H., A general model to account for enzyme variation in natural populations, Genetics 76 (1974) 837–848.
Yong, H.S., Allozyme variation in the melon fly Dacus cucurbitae (Insecta: Diptera: Tephritidae) from peninsular Malaysia, Comp. Biochem. Phys. B 102 (1992) 367–370. CrossRef
Ochando, M.D., Reyes, A., Genetic population structure in olive fly Bactrocera oleae (Gmelin): gene flow and patterns of geographic differentiation, J. Appl. Entomol. 124 (2000) 177–183. CrossRef
McPheron, B.A., Jorgensen, C.D., Berlocher, S.H., Low genetic variability in Utah cherry-infesting population of the apple maggot, Rhagoletis pomonella, Entomol. Exp. Appl. 46 (1988) 155–160. CrossRef
Huettel, M.D., Fuerst, P.A., Maruyama, T., Chakraborty, R., Genetic effects of multiple population bottlenecks in the Mediterranean fruit fly (Ceratitis capitata), Genetics 94 (1980) 47.
Loukas M., Population genetic studies of fruit flies of economic importance, especially medfly and olive fruit fly, using electrophoretic methods, in: Electrophoretic studies on agricultural pests, Loxdale H.D., Hollander J.D. (Eds.), Syst. Ass., Spec. Vol., Clarendon Press, Oxford, UK, 1989.
Baruffi, L., Damiani, G., Guglielmino, C.R., Bandi, C., Malacrida, A.R., Gasperi, G., Polymorphism within and between populations of Ceratitis capitata: comparison between RAPD and multilocus enzyme electrophoresis data, Heredity 74 (1995) 425–437. CrossRef
Marinkovic, D., Tucic, N., Moya, A., Ayala, F.J., Genetic diversity and linkage disequilibrium in Drosophila melanogaster with different rates of development, Genetics 117 (1987) 513–520.