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Evaluation of Angiotensin I-Converting Enzyme(ACE) inhibitory potential of some underutilized indigenous fruits ofWest Bengal using an in vitro model

Published online by Cambridge University Press:  12 November 2013

Susmita Das  and
Bratati De
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Abstract

Introduction. Angiotensin I-Converting Enzyme(ACE) is a key component in regulation of blood pressure by virtue ofthe rennin-angiotensin system. ACE converts the inactive decapeptide,angiotensin I, into the potent vasopressor octapeptide, angiotensinII, and inactivates bradykinin, which has a vasodilating action. So,inhibition of ACE has become a major target in control ofhypertension. It is well known that the consumption of fruits couldprovide health benefits by lowering the risk of chronic diseases suchas metabolic syndrome diseases including type 2 diabetes andcardiovascular disease. Our current study was focused on investigatingthe ACE inhibitory property of a few underutilized minor fruits ofWest Bengal using an in vitro assay. Materialsand methods. The potential antihypertensive activity ofunderutilized indigenous edible fruits of West Bengal, India, wasevaluated by their ability to inhibit Angiotensin-Converting Enzyme(ACE). The ACE inhibitory property was assayed using ACE from rabbitlung and hippuryl-histidyl-leucine as the substrate. Nineteen fruitspecies belonging to 15 families were investigated. The percentage ACEinhibitory activities of these fruits were studied at 20 µg freshweight of fruit extract per mL. The total phenol content of all thesefruits was determined following the standard Folin-Ciocalteu method.Total flavonoid content was also measured. Results. Theaqueous fruit extracts of the red variety of Trapabispinosa, Phoenix sylvestris,Cicca acida, Achras sapota andAverrhoa carambola presented more than 75% ACEinhibition. On the other hand, Punica granatum,Spondias pinnata, Trapabispinosa (green) and Ziziphus mauritianashowed about 50% inhibition. Aeglemarmelos, Annona squamosa, Annona reticulata,Feronia elephantum, Physalis peruviana andSyzygium jambos showed low activity (< 50%inhibition). Discussions. To date there has been noreport on Angiotensin I-Converting Enzyme inhibitory activities ofthese underutilized minor fruits of West Bengal, India. During ourstudy no correlation could be established between the % ACE inhibitionand the total phenol or flavonoid content of these fruit extracts. So,it appears that non-phenolic components may also be responsible forACE inhibitory activity. In our investigation we tried to establishthe fact that the consumption of these underutilized minor fruitsmight have potential in managing cardiovascular diseases.

Keywords

IndiaWest Bengalnatural resourcesfruit treesfruitsenzyme activityIndeBengale occidentalressource naturellearbre fruitierfruitsactivité enzymatiqueIndiaBengala Occidentalrecursos naturalesárboles frutalesfrutasactividad enzimática
Type
Original article
Information
Fruits , Volume 68 , Issue 6 , November 2013 , pp. 499 - 506
Copyright
© 2013 Cirad/EDP Sciences

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References

Gohlke, P., Linz, W., Scholkens, B.A., Kuwer, I., Bartenbach, S., Schnell, A., Unger, T., Angiotensin-converting enzyme inhibition improves cardiac function, Hypertension 23 (1994) 411–418.CrossRefGoogle ScholarPubMed
Hollenberg, N.K., Pharmacologic interrupting the rennin-angiotensin system, Annu. Rev. Pharmacol. Toxicol. 19 (1979) 559–582.CrossRefGoogle Scholar
Ondetti, M.A., Cushman, D.W., Enzyme of the renin-angiotensin system and their inhibitors, Annu. Rev. Biochem. 51 (1982) 283–308.CrossRefGoogle ScholarPubMed
Ukeda, H., Matsuda, H., Kuroda, H., Osajima, K., Matsufuji, H., Osajima, Y., Preparation and separation of angiotensin I converting enzyme inhibitory peptides, Nippon Nogeik. Kaishi 65 (1991) 1223–1228.CrossRefGoogle Scholar
Joshipura, K.J., Hu, F.B., Manson, J.E., Stampfer, M.J., Rimm, E.B., Speizer, F.E., Colditz, G., Ascherio, A., Rosner, B., Spiegelman, D., Willett, W.C., The effect of fruit and vegetable intake on risk for coronary heart disease, Ann. Intern. Med. 132 (2001) 1106–1114.CrossRefGoogle Scholar
Costacou, T., Mayer-Davis, E.J., Nutrition and prevention of type 2 diabetes, Annu. Rev. Nutr. 23 (2003) 147–170.CrossRefGoogle ScholarPubMed
Hung, H.C., Joshipura, K.J., Jiang, R., Hu, F.B., Hunter, D., Smith-Warner, S.A., Colditz, G.A., Rosner, B., Spiegelman, D., Willett, W.C., Fruit and vegetable intake and risk of major chronic disease, J. Natl. Cancer Inst. 96 (2004) 1577–1584.CrossRefGoogle Scholar
Landsberg, L., Molitch, M., Diabetes and hypertension: pathogenesis, prevention and treatment, Clin. Exp. Hypertens. 26 (2004) 621–628.CrossRefGoogle Scholar
Anon., WHO expert committee report, hypertension control, World Health Organ. (WHO) Tech. Rep. Series 862, Geneva, Switz., 1996, 1–83.
Johnston, J.I., Franz, V., Renin-angiotensin system: a dual tissue and hormonal system for cardiovascular control, J. Hypertens. 10 (1992) 13–26.CrossRefGoogle Scholar
Atkinson, A.B., Robertson, J.I.S., Captopril in the treatment of clinical hypertension and cardiac failure, Lancet 2 (1979) 836–839.CrossRefGoogle ScholarPubMed
Suh, H.J., Whang, J.H., Lee, H., Angiotensin I converting enzyme inhibitory peptide from corn gluten hydrolysate, Biotechnol. Letter 21 (1999) 1055–1058.CrossRefGoogle Scholar
Kohama, Y., Matsumoto, S., Oka, H., Teramoto, T., Okabe, M., Mimura, T., Isolation of an ACE-inhibitor from tuna muscle, Biochem. Biophys. Res. Commun. 156 (1988) 332–337.CrossRefGoogle Scholar
Funayama, S., Hikino, H., Hypotensive principles of Diospyros kaki leaves, Chem. Pharm. Bull. 27 (1979) 2865–2868.CrossRefGoogle ScholarPubMed
Kameda, K., Takaku, T., Okuda, H., Kimura, Y., Inhibitory effects of various flavonoids isolated from leaves of persimmon on angiotensin-converting enzyme activity, J. Nat. Prod. 50 (1987) 680–683.CrossRefGoogle ScholarPubMed
Park, P.J., Je, J.Y., Kim, S.K., Free radical scavenging activities of chitooligosaccharides by electron spin resonance spectrometry, J. Agric. Food Chem. 51 (2003) 4624–4627.CrossRefGoogle Scholar
Kang, D.G., Kim, Y.C., Sohn, E.J., Lee, Y.M., Lee, A.S., Yin, M.H., Lee, H.S., Hypotensive effect of butein via the inhibition of angiotensin converting enzyme, Biol. Pharm. Bull. 26 (2003) 1345–1347.CrossRefGoogle ScholarPubMed
Nyman, U., Joshi, P., Madsen, L.B., Pedersen, T.B., Pinstrup, M., Rajasekharan, S., George, V., Pushpangadan, P., Ethnomedical information and in vitro screening for angiotensin-converting enzyme inhibition of plants utilized as traditional medicines in Gujarat, Rajasthan and Kerala, J. Ethnopharmacol. 60 (1998) 247–263.CrossRefGoogle ScholarPubMed
Das, S., Das, S., De, B., In vitro inhibition of key enzymes related to diabetes by the aqueous extracts of some fruits of West Bengal, India, Curr. Nutr. Food Sci. 8 (2012) 19–24.Google Scholar
Cushman, D.W., Cheung., H.S., Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung, Biochem. Pharmacol. 20 (1971) 1637–1648.CrossRefGoogle ScholarPubMed
Sadasivam S., Manikam A., Biochemical methods, Wiley Eastern Lrd., India, 1992.
Kim, D., Jeong, S.W., Lee, C.Y., Antioxidant capacity of phenolic phytochemicals from various cultivars of plums, Food Chem. 81 (2003) 321–326.CrossRefGoogle Scholar
Balasuriya, B.W.N., Rupasinghe, H.P.V., Plant flavonoids as angiotensin converting enzyme inhibitors in regulation of hypertension, Func. Foods Health Dis. 5 (2011) 172–188.Google Scholar
Tossou, C.C., Floquet, A.B., Sinsin, B.A., Relationship between production and consumption of fruit grown on the Allada plateau in southern Benin, Fruits 67 (1) (2012) 3–12.CrossRefGoogle Scholar
Kyung, A.J., Tae, C.S., Daeseok, H., In, H.K., Young, E.K., Chang, H.L., Cardiovascular protective properties of kiwifruit extracts in vitro, Biol. Pharm. Bull. 28 (9) (2005) 1782—1785.Google Scholar
Zahra, F.H., Mostafa, J., Ahmad, T., Mohammad, F., The antihypertensive and vasodilator effects of aqueous extract from Berberis vulgaris fruit on hypertensive rats, Phytother. Res. 19 (3) (2005) 222–225.Google Scholar
Verma, S.K., Jain, V., Katewa, S.S., Blood pressure lowering, fibrinolysis enhancing and antioxidant activities of cardamom (Elettaria cardamomum), Indian J. Biochem. Biophys. 46 (6) (2009) 503–506.Google Scholar
Ayub, M.Y., Norazmir, M.N., Mamot, S., Jeeven, K., Hadijah, H., Anti-hypertensive effect of pink guava (Psidium guajava) puree on spontaneous hypertensive rats, Int. Food Res. J. 17 (2010) 89–96.Google Scholar
Basu, A., Penugonda, K., Pomegranate juice: a heart-healthy fruit juice, Nutr. Rev. 67 (1) (2009) 49–56.CrossRefGoogle ScholarPubMed
Narasimhacharya, A.V.R.L., Rupal, A.V., Prajapati, P.C., Angiotensin Converting Enzyme inhibition by certain fruits: an in vitro study, Curr. Trends Biotechnol. Pharm. 4 (3) (2010) 801–808.Google Scholar
Pinto, M.D.S., Kwon, Y.I., Apostolidis, E., Lajolo, F.M., Genovese, M.I.E., Shetty, K., Functionality of bioactive compounds in Brazilian strawberry (Fragaria × ananassa Duch.) cultivars: Evaluation of hyperglycemia and hypertension potential using in vitro models, J. Agric. Food Chem. 56 (2008) 4386–4392.CrossRefGoogle Scholar
Atato, A., Wala, K., Dourma, M., Bellefontaine, R., Woegan, Y.A, Batawila, K., Akpagana, K., Lianas of Togo bearing edible fruit, Fruits 67 (5) (2012) 353–368.CrossRefGoogle Scholar
Dadjo, C., Assogbadjo, A.E., Fandohan, B., Kakai, R.G., Chakeredza, S., Houehanou, T.D., Damme, P. van, Sinsin, B., Uses and management of black plum (Vitex doniana Sweet) in Southern Benin, Fruits 67 (4) (2012) 239–248.CrossRefGoogle Scholar