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Isolation of Cylindrocladium spp. in roots and soils from banana cropping systems

Published online by Cambridge University Press:  24 January 2008

Jean-Michel Risède*
Affiliation:
CIRAD, UPR Systèmes bananes et ananas, Station de Neufchâteau Sainte-Marie, 97130 Capesterre-Belle-Eau, Guadeloupe
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Abstract

Introduction. This protocol aims at detecting Cylindrocladium spp. in root and soil samples from banana fields and at quantifying the inoculum. The principle of the two methods applied [Root Slice Plating Method (RSPM) and Soil Isolation Method (SIM)], the key advantages of these methods, starting plant and soil materials, the time required and the expected results are presented. Materials and methods. Necessary laboratory materials, and details of the six steps achieved for the use of the two methods are described. Results. When using the RSPM, typical Cylindrocladium colonies are isolated in petri dishes. When using the SIM, typical Cylindrocladium colonies originate from microsclerotia embedded or not in banana root tissue. The inoculum density can be calculated.

Type
Research Article
Copyright
© CIRAD, EDP Sciences, 2008

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References

Phipps, P.M., Beute, M.K., Barker, K.R., An elutriation method for quantitative isolation of Cylindrocladium crotalariae microsclerotia from peanut field soil, Phytopathol. 66 (1976) 1255–1259. CrossRef
Risède, J.-M., Spatial and temporal distribution in soils of Cylindrocladium sp. fungal pathogen of bananas, Phytopathol. 85 (1995) 1563 (abstract).
Crous P.W., Taxonomy and pathology of Cylindrocladium (Calonectria) and allied genera, Am. Phytopathol. Soc. (APS Press), St. Paul, USA, 2002, 294 p.