Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-27T10:02:42.213Z Has data issue: false hasContentIssue false
  • English
  • Français

Long-term maintenance of Cylindrocladium strains and procedures for inoculum production

Published online by Cambridge University Press:  15 May 2008

Jean-Michel Risède  and
Béatrice Rhino
Jean-Michel Risède
Affiliation:
CIRAD-Persyst, UPR Systèmes bananes et ananas, Station de Neufchâteau, Sainte-Marie, 97130 Capesterre-Belle-Eau, Guadeloupe beatrice.rhino@cirad.fr
Béatrice Rhino
Affiliation:
CIRAD-Persyst, UPR Systèmes bananes et ananas, Station de Neufchâteau, Sainte-Marie, 97130 Capesterre-Belle-Eau, Guadeloupe beatrice.rhino@cirad.fr
Get access

Abstract

Introduction. The techniques presented allow a long-term conservation of Cylindrocladium strains to preserve them from major morphological and physiological changes. The principle of the methods applied, key advantages, starting plant material, time required and expected results are presented. Materials and methods. The necessary laboratory materials, and details of the nine steps achieved for the monospore purification of Cylindrocladium strains, their long-term maintenance, and the production of conidia and microsclerotia are described. Results. When revived, purified strains generate actively growing colonies within 48–72 h. Such colonies can then be readily used for conidia or microsclerotia production.

Keywords

France (Guadeloupe)Musa sp.disease controlmethodslaboratory equipmentCylindrocladium spp.biological preservationinoculationFrance (Guadeloupe)Musa sp.contrôle de maladiesméthodematériel de laboratoireCylindrocladium spp.conservation biologiqueinoculation
Type
Research Article
Information
Fruits , Volume 63 , Issue 3 , May 2008 , pp. 193 - 197
Copyright
© CIRAD, EDP Sciences, 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Risède, J.-M., Isolation of Cylindrocladium spp. in roots and soils from banana cropping systems, Fruits 63 (2008) 57–61. CrossRef
Risède, J-M., Éléments de caractérisation de Cylindrocladium sp. agent de nécroses racinaires du bananier en Martinique, Fruits 49 (1994) 167–178.
Fisher, N.L., Burgess, L.W., Toussoun, T.A., Nelson, P.E., Carnation leaves as a substrate and for preserving cultures of Fusarium species, Phytopathology 72 (1982) 151–153. CrossRef
Crous, P.W., Philipps, A.J.L., Wingfield, M.J., Effects of cultural conditions on vesicle and conidium morphology in species of Cylindrocladium and Cylindrocladiella, Mycologia 84 (1992) 497–504. CrossRef