Hostname: page-component-745bb68f8f-kw2vx Total loading time: 0 Render date: 2025-01-31T04:44:55.879Z Has data issue: false hasContentIssue false
  • English
  • Français

The comparison of closely related groups of rodents by DNA/DNA annealing

Published online by Cambridge University Press:  14 April 2009

Anne McLaren  and
P. M. B. Walker
Anne McLaren
Affiliation:
A.R.G. Unit of Animal Genetics, Institute of Animal Genetics, Edinburgh
P. M. B. Walker
Affiliation:
Department of Zoology, Edinburgh University
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Within the genus Apodemus, DNA from A. sylvaticus and A. flavicollis could be distinguished on the basis of reciprocal DNA/DNA annealing in agar, even when unfractionated DNA was used. In preliminary experiments, DNA from geographical races of A. sylvaticus could not be distinguished, nor could DNA from two closely related species of Peromyscus.

Within the species Mus musvulus, the fast-renaturing fraction of L-cell DNA, separated on a hydroxyapatite column, was able to discriminate between L-cell (originating from the C3H inbred strain) and NZB (New Zealand Black) inbred strain DNA in agar.

Type
Research Article
Information
Genetics Research , Volume 12 , Issue 2 , October 1968 , pp. 117 - 124
Copyright
Copyright © Cambridge University Press 1968

References

REFERENCES

Berry, R. J., Evans, I. M. & Sennitt, B. F. C. (1967). The relationships and ecology of Apodemus sylvaticus from the Small Isles of the Inner Hebrides, Scotland. J. Zool. 152, 333–346.CrossRefGoogle Scholar
Hoyer, B. H., McCarthy, B. J. & Bolton, E. T. (1964). A molecular approach in the systematics of higher organisms. Science 144, 959–967.CrossRefGoogle Scholar
Jewell, P. A. & Fullagar, P. J. (1965). Fertility among races of the field mouse (Apodemus sylvaticus) and their failure to form hybrids with the yellow-necked mouse (Apodemus flavicollis). Evolution 19, 175–181.Google Scholar
McLaben, A. & Walker, P. M. B. (1965). Genetic discrimination by means of DNA/DNA binding. Genet. Res. 6, 230–247.CrossRefGoogle Scholar
McLaren, A. & Walker, P. M. B. (1966). Discriminating power of rodent deoxyribonucleic acid on incubation in agar. Nature, Lond. 211, 486–490.CrossRefGoogle ScholarPubMed
Martin, M. A. & Hoyer, B. H. (1967). Adenine plus thymine and guanine plus cytosine enriched fractions of animal DNA's as indicators of polynucleotide homologies. J. mol. Biol. 27, 113–129.CrossRefGoogle ScholarPubMed
McCallum, M. & Walker, P. M. B. (1967). Hydroxyapatite fractionation procedures in the study of the mammalian genome. Biochem. J. 105, 163–169.CrossRefGoogle Scholar
Miyazawa, Y. & Thomas, C. A. (1965). The nucleotide composition of short segments of DNA molecules. J. mol. Biol. 11, 223–237.CrossRefGoogle ScholarPubMed
Popp, R. A. (1967). Hemoglobins of mice: sequence and possible ambiguity at one position of the alpha chain. J. mol. Biol. 27, 9.CrossRefGoogle ScholarPubMed
Walker, P. M. B. & Mclaren, A. (1965 a) Specific duplex formation in vitro of mammalian DNA. J. mol. Biol. 12, 394–409.CrossRefGoogle ScholarPubMed
Walker, P. M. B. & McLaren, A. (1965 b). Fractionation of mouse deoxyribonucleic acid on hydroxyapatite. Nature, Lond. 208, 1175–1179.CrossRefGoogle ScholarPubMed