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Isolation and Antimicrobial Resistance of Staphylococcus aureus Isolates in a Dental Clinic Environment

Published online by Cambridge University Press:  02 January 2015

Rogério Heládio Lopes Motta ,
Francisco Carlos Groppo ,
Cristiane de Cássia Bergamaschi ,
Juliana Cama Ramacciato ,
Sinvaldo Baglie  and
Thales Rocha de Mattos-Filho
Rogério Heládio Lopes Motta*
Affiliation:
Department of Physiological Sciences, Area of Pharmacology, São Leopoldo Mandic, Campinas, Brazil
Francisco Carlos Groppo
Affiliation:
Department of Physiological Sciences, Area of Pharmacology, Dentistry School of Piracicaba, University of Campinas, Piracicaba, Brazil
Cristiane de Cássia Bergamaschi
Affiliation:
Department of Physiological Sciences, Area of Pharmacology, Dentistry School of Piracicaba, University of Campinas, Piracicaba, Brazil
Juliana Cama Ramacciato
Affiliation:
Department of Physiological Sciences, Area of Pharmacology, São Leopoldo Mandic, Campinas, Brazil
Sinvaldo Baglie
Affiliation:
Department of Pharmaceutical Sciences, Ponta Grossa University, Paraná, Brazil
Thales Rocha de Mattos-Filho
Affiliation:
Department of Physiological Sciences, Area of Pharmacology, Dentistry School of Piracicaba, University of Campinas, Piracicaba, Brazil
*
Dept.t of Physiologic Sciences, Dental School of Piracicaba, University of Campinas, Av. Limeira 901, Areião CEP 13.414-903, Piracicaba, São Paulo, Brazil (rogeriomotta@fop.unicamp.br)
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Abstract

Objective.

To determine the number of Staphylococcus aureus isolates collected in a dental clinical environment and to determine their susceptibility to antimicrobial agents commonly used in dentistry.

Setting.

Undergraduate clinic of the Dental School of Piracicaba, University of Campinas, Brazil.

Methods.

Sterile cotton swabs were used to collect the samples from dental-chair push buttons, light handles, 3-in-l syringes, computer “Enter” keys, doorknobs, and X-ray tubes before, during, and after clinical procedures. These samples were spread on brain-heart infusion agar and were incubated at 37°C for 24 hours. The resulting S. aureus isolates were counted and classified using Gram staining and biochemical tests. The counts among the 3 periods and the groups were analyzed by Kruskal-Wallis and Dunn tests (α = 5%). Commercial paper disks containing widely prescribed antimicrobial agents (β-lactams, macrolides, clindamycin, and vancomycin) were used to perform the antimicrobial susceptibility tests.

Results.

An increase in the number of microorganisms was observed during clinical procedures (P < .05). The highest bacterial resistance rates were observed for the β-lactam group. All isolated strains were sensitive to vancomycin, and 2% of them were resistant to methicillin.

Conclusions.

Clinical procedures increased the number and proportion of antimicrobial-resistant S. aureus isolates dispersed in a dental clinical environment. The present study highlights the need to establish strategies to prevent emergence of drug-resistant bacterial strains in dental settings.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 28 , Issue 2 , February 2007 , pp. 185 - 190
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2007

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