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Comparison of Pulsed-Field Gel Electrophoresis and Amplified Fragment-Length Polymorphism for Epidemiological Investigations of Common Nosocomial Pathogens

Published online by Cambridge University Press:  02 January 2015

Erika M.C. D'Agata ,
Monique M. Gerrits ,
Yi-Wei Tang ,
M. Samore  and
Johannes G. Kusters
Erika M.C. D'Agata*
Affiliation:
Division of Infectious Diseases, Vanderbilt University, Nashville, Tennessee
Monique M. Gerrits
Affiliation:
Department of Medical Microbiology, Free University, Amsterdam, The Netherlands
Yi-Wei Tang
Affiliation:
Division of Infectious Diseases, Vanderbilt University, Nashville, Tennessee
M. Samore
Affiliation:
Division of Infectious Diseases, IDS Hospital, Salt Lake City, Utah
Johannes G. Kusters
Affiliation:
Department of Medical Microbiology, Free University, Amsterdam, The Netherlands
*
Beth Israel Deaconess Medical Center, Division of Infectious Diseases, Kennedy 6A, 330 Brookline Ave, Boston, MA 02215
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Abstract

Objective:

To compare molecular typing by amplified fragment-length polymorphism (AFLP) analysis with pulsed-field gel electrophoresis (PFGE) with respect to the ability to differentiate between epidemiologically related and unrelated isolates of common nosocomial pathogens recovered during a period of endemicity.

Design:

Retrospective laboratory analysis.

Setting:

Tertiary-care institution.

Methods:

17 isolates of Acinetobacter baumannii, 22 isolates of Pseudomonas aeruginosa, and 22 vancomycin-resistant Enterococcus faecium (VRE) were typed by both methods.

Results:

AFLP generated comparable results to PFGE for A baumannii and P aeruginosa isolates; both methods identified epidemiologically related and unrelated isolates. However, strain typing of VRE isolates produced discordant results between the two methods. PFGE identified 10 different strain types and differentiated between all epidemiologically related and unrelated isolates. In contrast, AFLP generated only five different strain types, three of which contained both epidemiologically related and unrelated isolates.

Conclusion:

Molecular typing by AFLP is comparable to PFGE for A baumannii and P aeruginosa isolates. For VRE isolates, however, PFGE remains the method of choice.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 22 , Issue 9 , September 2001 , pp. 550 - 554
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2001

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