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Disinfectant Susceptibility Profiling of Glutaraldehyde-Resistant Nontuberculous Mycobacteria

Published online by Cambridge University Press:  02 May 2017

Winona Burgess ,
Alyssa Margolis ,
Sara Gibbs ,
Rafael Silva Duarte  and
Mary Jackson
Winona Burgess
Affiliation:
Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States
Alyssa Margolis
Affiliation:
Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States
Sara Gibbs
Affiliation:
Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States
Rafael Silva Duarte
Affiliation:
Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. (Present affiliation: Department of Chemistry and Biochemistry, University of Colorado-Boulder, Colorado [S.G.].)
Mary Jackson*
Affiliation:
Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States
*
Address correspondence to Mary Jackson, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523-1682 (Mary.Jackson@colostate.edu).
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Abstract

OBJECTIVE

Activated alkaline glutaraldehyde (GTA) remains one of the most widely used high-level disinfectants worldwide. However, several reports have highlighted the potential for nontuberculous mycobacteria to develop high-level resistance to this product. Because aldehyde resistance may lead to cross-resistance to other biocides, we investigated the susceptibility profile of GTA-resistant Mycobacterium chelonae and M. abscessus isolates to various disinfectant chemistries.

METHODS

High-level disinfectants commonly used in the reprocessing of endoscopes and other heat-sensitive, semicritical medical equipment, including different formulations of aldehyde-based products and oxidizing agents, were tested against 10 slow- and fast-growing, GTA-susceptible and GTA-resistant, Mycobacterium isolates in suspension tests and carrier tests at different temperatures.

RESULTS

While peracetic acid– and hydrogen peroxide–based disinfectants (S40, Resert XL, Reliance DG) efficiently killed all of the Mycobacterium isolates, GTA- and ortho-phthalaldehyde-based products (ie, Cidex, Aldahol, Cidex OPA) showed variable efficacy against GTA-resistant strains despite the ability of some formulations (Aldahol) to overcome the resistance of some of these isolates, especially when the temperature was increased from 20°C to 25°C.

CONCLUSIONS

Application permitting, oxidizing chemistries may provide a safe alternative to aldehyde-based products, particularly in GTA-resistant mycobacterial outbreaks.

Infect Control Hosp Epidemiol 2017;38:784–791

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 38 , Issue 7 , July 2017 , pp. 784 - 791
Copyright
© 2017 by The Society for Healthcare Epidemiology of America. All rights reserved 

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