Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-27T08:01:51.719Z Has data issue: false hasContentIssue false

Evidence for the Genetic Unrelatedness of Nosocomial Alcaligenes xylosoxidans Strains in a Pediatric Hospital

Published online by Cambridge University Press:  02 January 2015

Abstract

Restriction fragment-length polymorphism of ribosomal DNA and random amplified polymorphic DNA analysis were used to study the relationships among nine isolates of Alcaligenes xylosoxidans obtained from seven children in a pediatric hospital. All the children harbored only genotypically unrelated strains, thus excluding a common source of exposure or patient-to-patient strain transfer.

Type
Concise Communications
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1997

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Cheron, M, Abachin, E, Guerot, E, El-Bez, E, Simonet, M. Investigation of hospital-acquired infections due to Alcaligenes xylosoxidans by DNA restriction fragment length polymorphism. J Clin Microbiol 1994;32:10231026.CrossRefGoogle Scholar
2. Reverdy, ME, Freney, J, Coulet, M, Surgot, M, Marmet, D, Ploton, C. Nosocomial colonization and infection by Achromobacter xylosoxidans . J Clin Microbiol 1984;26:140143.CrossRefGoogle Scholar
3. Williams, JGK, Kubelik, AR, Livak, KJ, Rafalski, JA, Tingey, SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 1990;18:65316535.CrossRefGoogle ScholarPubMed
4. Welsh, J, McClelland, M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 1990;18:72137218.CrossRefGoogle ScholarPubMed
5. Bingen, E, Boissinot, C, Desjardins, P, et al. Arbitrarily primed polymerase chain reaction provides rapid differentiation of Proteus mirabilis isolates from a pediatric hospital. J Clin Microbiol 1993;31:10551059.CrossRefGoogle ScholarPubMed
6. Bingen, E, Denamur, E, Elion, J. Use of ribotyping in epidemiological surveillance of nosocomial outbreaks. Clin Microbiol Rev 1994;7:311327.Google Scholar
7. Boukadida, J, Monastiri, K, Snoussi, N, Jeddi, M, Berche, P. Nosocomial neonatal meningitidis by A xylosoxidans transmitted by aqueous eosin. Pediatr Infect Dis J 1993;12:696697.Google Scholar
8. Cavé, H, Bingen, E, Elion, J, Denamur, E. Differentiation of Escherichia coli strains using randomly amplified polymorphic DNA analysis. Res Microbiol 1994;145:141150.Google Scholar