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Gentamicin-Resistance Plasmids in an Intensive Care Unit

Published online by Cambridge University Press:  02 January 2015

David M. Shlaes ,
Charlotte Currie-McCumber ,
Mabel Eanes ,
Grace Rotter  and
Rachel Floyd
David M. Shlaes*
Affiliation:
Division of Infectious Disease, Case Western University School of Medicine, Research, Nursing, and Laboratory Services, Veterans Administration Medical Center, Cleveland, Ohio
Charlotte Currie-McCumber
Affiliation:
Division of Infectious Disease, Case Western University School of Medicine, Research, Nursing, and Laboratory Services, Veterans Administration Medical Center, Cleveland, Ohio
Mabel Eanes
Affiliation:
Division of Infectious Disease, Case Western University School of Medicine, Research, Nursing, and Laboratory Services, Veterans Administration Medical Center, Cleveland, Ohio
Grace Rotter
Affiliation:
Division of Infectious Disease, Case Western University School of Medicine, Research, Nursing, and Laboratory Services, Veterans Administration Medical Center, Cleveland, Ohio
Rachel Floyd
Affiliation:
Division of Infectious Disease, Case Western University School of Medicine, Research, Nursing, and Laboratory Services, Veterans Administration Medical Center, Cleveland, Ohio
*
Infectious Diseases and Microbiology, Cleveland Veterans Administration Medical Center, 10701 East Boulevard, Cleveland, OH 44106
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Abstract

To better understand the role of plasmids and their importance in the endemic antibiotic resistance of Enterobacteriaceae, we began a prospective study of our combined medical intensive care/coronary care unit. An initial culture survey of the patients, ward staff, and environment was followed by a prospective sampling of 139 consecutive new admissions at the time of admission to the unit, and at regular intervals thereafter for the remainder of their stay. All cultures were planted on agar-containing gentamicin. Of the 147 patients studied, 12 (8.2%) were colonized with 20 strains of gentamicin-resistant gram-negative bacilli (GRGNB) at 29 sites. An additional four GRGNB were isolated from the environment. Of the 24 GRGNB strains, 7 (29%) Enterobacteriaceae carried plasmids shown to carry the gentamicin-resistance determinant. Plasmids were further characterized by restriction endonuclease digestion profiles of plasmid DNA purified from E. coli C600 transconjugants or transformants. A 93 kb plasmid introduced to the unit by a Serratia liquefaciens colonizing a patient transferred from another area in the hospital was identical to 93 kb plasmids carried by a C. amalonaticus and an E. aerogenes subsequently colonizing another patient on the unit. A 60 kb plasmid, first isolated from a S. marcescens colonizing a sink drain was later isolated from an E. cloacae colonizing a patient. Our results indicate that spread of specific R-plasmids may be one mechanism for dissemination of antibiotic resistance on our MICU/CCU.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 7 , Issue 7 , July 1986 , pp. 355 - 361
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1986

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References

1.Shlaes, DM, Currie, CA, Rotter, G, et al: The epidemiology of gentamicin-resistant gram-negative bacillary colonization on a spinal cord injury unit. J Clin Microbiol 1983;18:227–235.CrossRefGoogle ScholarPubMed
2.Shlaes, DM, Currie, CA: Endemic gentamicin-resistance R-factors on a spinal cord injury unit. J Clin Microbiol 1983;18:236–241.CrossRefGoogle ScholarPubMed
3.Shlaes, DM, Currie, CA, Vartian, CV: Variability in DNA sequence of closely related nosocomial gentamicin-resistance plasmids. J Infect Dis 1983;148:1013–1018.CrossRefGoogle Scholar
4.Tompkins, LS, Plorde, JJ, Falkow, S: Molecular analysis of R factors from multiresistant nosocomial isolates. J Infect Dis 1980;141:625–636.CrossRefGoogle ScholarPubMed
5.Myers, JA, Sanchez, D, Elwell, LP, et al: Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol 1976;127:1529–1537.CrossRefGoogle Scholar
6.Courtney, M-A, Miller, JR, Summersgill, J, et al: R-factor responsible for an outbreak of multiply antibiotic-resistant Klebsiella pneumoniae. Antimicrob Agents Chemother 1980;18:926–929.CrossRefGoogle ScholarPubMed
7.John, JF, McNeill, WF: Characteristics of Serratia marcescens containing a plasmid for gentamicin resistance in nosocomial infections. J Infect Dis 1981;143:810–817.CrossRefGoogle ScholarPubMed
8.Markowitz, SM, Veazey, JM, Macrina, FL, et al: Sequential outbreaks of infection due to Klebsiella pneumoniae in a neonatal intensive care unit: Implication of aconjugative R plasmid. J Infect Dis 1980;142:106–112.CrossRefGoogle Scholar
9.Rubens, CE, Farrar, WE Jr, McGee, ZA, et al: Evolution of a plasmid mediating resistance to multiple antimicrobial agents during a prolonged epidemic of nosocomial infections. J Infect Dis 1981;143:170–181.CrossRefGoogle ScholarPubMed
10.Sadowski, PL, Peterson, BC, Gerding, DN, et al: Physical characterization of ten R plasmids obtained from an outbreak of nosocomial Klebsiella pneumoniae infections. Antimicrob Agents Chemother 1979;15:616–624.CrossRefGoogle Scholar
11.Shlaes, DM, Medeiros, A, Currie-McCumber, C, et al: A novel plasmid-medi-ated ß-lactamase in Enterobacteriaceae from Ohio. Antimicrob Agents Chemother, in press.Google Scholar
12.Shlaes, DM, Currie McCumber, C: Commentary: Dissemination of a plasmid determining multiple antibiotic resistance between two Veterans Administration medical centers. Infect Control 1986;7:362–364.CrossRefGoogle Scholar